Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   L897_RS00675 Genome accession   NC_021807
Coordinates   106134..106568 (+) Length   144 a.a.
NCBI ID   WP_020833199.1    Uniprot ID   -
Organism   Streptococcus pyogenes HSC5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101134..111568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L897_RS00645 (L897_00670) - 102803..103168 (+) 366 WP_002986560.1 DUF1033 family protein -
  L897_RS00650 (L897_00675) comYA 103261..104199 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  L897_RS00655 (L897_00680) comYB 104135..105169 (+) 1035 WP_236608388.1 competence type IV pilus assembly protein ComGB Machinery gene
  L897_RS00660 (L897_00685) comYC 105171..105497 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  L897_RS00665 (L897_00690) comGD 105472..105900 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  L897_RS00670 (L897_00695) comGE 105857..106141 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  L897_RS00675 (L897_00700) comYF 106134..106568 (+) 435 WP_020833199.1 competence type IV pilus minor pilin ComGF Machinery gene
  L897_RS00680 (L897_00705) comGG 106552..106878 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  L897_RS00685 (L897_00710) comYH 106976..107929 (+) 954 WP_042361463.1 class I SAM-dependent methyltransferase Machinery gene
  L897_RS00690 (L897_00715) - 107988..109184 (+) 1197 WP_020833201.1 acetate kinase -
  L897_RS09080 (L897_00720) - 109371..109679 (+) 309 Protein_92 hypothetical protein -
  L897_RS00695 (L897_00725) proC 109762..110532 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16670.28 Da        Isoelectric Point: 10.2526

>NTDB_id=59906 L897_RS00675 WP_020833199.1 106134..106568(+) (comYF) [Streptococcus pyogenes HSC5]
MSKQLSNIKAFTLLDALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVTDNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=59906 L897_RS00675 WP_020833199.1 106134..106568(+) (comYF) [Streptococcus pyogenes HSC5]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGATGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAACTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.36

96.528

0.486
  comYF Streptococcus mutans UA159

49.64

96.528

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

43.284

93.056

0.403
  comGF/cglF Streptococcus pneumoniae Rx1

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae D39

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae R6

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae TIGR4

42.105

92.361

0.389
  comGF/cglF Streptococcus mitis SK321

41.353

92.361

0.382

Multiple sequence alignment