Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5R88_RS16475 Genome accession   NZ_CP081943
Coordinates   3634254..3635954 (+) Length   566 a.a.
NCBI ID   WP_223437600.1    Uniprot ID   -
Organism   Pseudomonas sp. MM213     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3629254..3640954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5R88_RS16455 (K5R88_16350) - 3629392..3632340 (+) 2949 WP_226298059.1 DUF748 domain-containing protein -
  K5R88_RS16460 (K5R88_16355) - 3632340..3632636 (+) 297 WP_008033199.1 DUF2845 domain-containing protein -
  K5R88_RS16465 (K5R88_16360) - 3632931..3633284 (-) 354 WP_008042835.1 BON domain-containing protein -
  K5R88_RS16470 (K5R88_16365) - 3633612..3634028 (-) 417 WP_008033203.1 pilin -
  K5R88_RS16475 (K5R88_16370) pilB 3634254..3635954 (+) 1701 WP_223437600.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5R88_RS16480 (K5R88_16375) pilC 3635957..3637174 (+) 1218 WP_192227988.1 type II secretion system F family protein Machinery gene
  K5R88_RS16485 (K5R88_16380) pilD 3637176..3638048 (+) 873 WP_192419974.1 A24 family peptidase Machinery gene
  K5R88_RS16490 (K5R88_16385) coaE 3638045..3638668 (+) 624 WP_008033209.1 dephospho-CoA kinase -
  K5R88_RS16495 (K5R88_16390) yacG 3638665..3638865 (+) 201 WP_008033210.1 DNA gyrase inhibitor YacG -
  K5R88_RS16500 (K5R88_16395) - 3638874..3639089 (-) 216 WP_008033212.1 hypothetical protein -
  K5R88_RS16505 (K5R88_16400) - 3639154..3639843 (-) 690 WP_192227990.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62592.67 Da        Isoelectric Point: 5.9727

>NTDB_id=598584 K5R88_RS16475 WP_223437600.1 3634254..3635954(+) (pilB) [Pseudomonas sp. MM213]
MNDIALSGLAKQLVLAELLTDQSAQQAYQQAQRHKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHASGLEDMADVDLDG
MDIESIDDHQQDAIAGQDADDAPVVRFVHKMLLDAIRSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNAKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSQCKKPIDIPREALIKEGFPE
ERIGTFTIYEPVGCDQCNGGYKGRVGIYEVVKNTAGLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=598584 K5R88_RS16475 WP_223437600.1 3634254..3635954(+) (pilB) [Pseudomonas sp. MM213]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTTGCCGAGCTGCTCACCGACCAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGTCATAAAATCTCCCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAAAGCCGCCAGGTCG
CCGAGATCGCCTCGGAGCATTTCGGCATGGCACTGCTCGACCTCAACTGCCTGGACAAGGAAACCCAACCCAAGGGCCTG
GTCAGCGAAAAACTGGTTCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGTGGCAACAAGTTGTTCGTGGGGGTTTCCGA
CCCCACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACCGGGCTGAACACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAGAAGTTCTTCGACAGCCATGCCAGCGGACTGGAAGACATGGCCGACGTCGACCTCGATGGC
ATGGACATCGAGTCGATCGATGACCACCAACAGGACGCCATCGCCGGACAGGATGCCGACGACGCGCCGGTGGTGCGCTT
CGTTCACAAGATGCTGCTGGACGCGATCAGGAGTGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGTGAAGTCGCCAAGCCGCCGATTCAACTGGCCAACCGCATCGCCGCCCGCCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
ATCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCATCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTACGAGCCGGACCAGAAAGACCTCTACATGGCCGCGCTGAAGCAGCCGCAAGGG
ATGATTCTGGTGACCGGCCCCACGGGGTCGGGCAAGACCGTGTCGCTCTACACCGGGCTGAACATACTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTCGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACGCCAAGCAGG
GACTGGGTTTTGCCCAGGCGCTGCGCTCCTTCTTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCATCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGGTTCAACATCGCGACGTCCGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCAGTGCAAGAAGCCCATCGATATTCCCCGCGAGGCATTGATCAAGGAAGGCTTCCCAGAG
GAACGGATTGGCACATTCACGATCTATGAGCCGGTCGGTTGCGATCAGTGCAACGGCGGCTACAAGGGGCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACAGCAGGCCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGATATCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGTACTTCCGGGCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.938

99.823

0.588
  pilB Acinetobacter baumannii D1279779

58.511

99.647

0.583
  pilB Legionella pneumophila strain ERS1305867

56.814

99.823

0.567
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.98

99.117

0.505
  pilB Vibrio campbellii strain DS40M4

49.474

100

0.498
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.463

0.367