Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   K5P74_RS07420 Genome accession   NZ_CP081846
Coordinates   1490900..1491505 (-) Length   201 a.a.
NCBI ID   WP_222225395.1    Uniprot ID   -
Organism   Enterococcus raffinosus strain CX012922     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1485900..1496505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5P74_RS07400 (K5P74_07400) - 1486470..1488572 (-) 2103 WP_222225393.1 DNA internalization-related competence protein ComEC/Rec2 -
  K5P74_RS07405 (K5P74_07405) - 1488695..1489186 (-) 492 WP_010744907.1 ComE operon protein 2 -
  K5P74_RS07410 (K5P74_07410) - 1489312..1489452 (+) 141 WP_222225394.1 hypothetical protein -
  K5P74_RS07415 (K5P74_07415) - 1489555..1490640 (-) 1086 WP_261321614.1 MFS transporter -
  K5P74_RS07420 (K5P74_07420) comEA 1490900..1491505 (-) 606 WP_222225395.1 helix-hairpin-helix domain-containing protein Machinery gene
  K5P74_RS07425 (K5P74_07425) sepM 1491610..1492653 (-) 1044 WP_222225396.1 SepM family pheromone-processing serine protease Regulator
  K5P74_RS07430 (K5P74_07430) coaD 1492643..1493137 (-) 495 WP_010744912.1 pantetheine-phosphate adenylyltransferase -
  K5P74_RS07435 (K5P74_07435) rsmD 1493139..1493693 (-) 555 WP_010744913.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  K5P74_RS07440 (K5P74_07440) - 1493853..1494200 (-) 348 WP_028020682.1 YlbG family protein -
  K5P74_RS07445 (K5P74_07445) - 1494187..1494612 (-) 426 WP_222226887.1 YlbF family regulator -
  K5P74_RS07450 (K5P74_07450) - 1494614..1495720 (-) 1107 WP_222225397.1 CAP-associated domain-containing protein -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 22368.28 Da        Isoelectric Point: 4.4221

>NTDB_id=597711 K5P74_RS07420 WP_222225395.1 1490900..1491505(-) (comEA) [Enterococcus raffinosus strain CX012922]
MKEYLKKYPYWLAVPAILAVILFFFFSRTSEEELTDPAVYSSESSKVETSESINQEWYVDVKGAVKKTGMYRISKGMRLM
DAIDLAGGFTAEADQNQINFSKLLSDQEIIYVPKVGEEIPRLKEATVESDGSIGGESQEKININTADATELQKLSGIGEK
KATDIIKYREENGSFNAIDDLTKVSGIGEKTLENLKDSITI

Nucleotide


Download         Length: 606 bp        

>NTDB_id=597711 K5P74_RS07420 WP_222225395.1 1490900..1491505(-) (comEA) [Enterococcus raffinosus strain CX012922]
ATGAAAGAGTATTTAAAAAAGTATCCTTACTGGCTAGCAGTACCGGCTATTCTTGCAGTTATTCTTTTTTTCTTCTTTTC
CAGAACATCTGAAGAAGAGCTAACAGATCCGGCCGTTTATTCTTCAGAGAGCAGTAAGGTAGAAACAAGTGAATCTATCA
ATCAAGAGTGGTATGTCGATGTCAAAGGAGCGGTCAAAAAAACAGGAATGTATCGTATTTCAAAAGGCATGCGTCTAATG
GATGCTATTGATCTTGCAGGCGGTTTTACAGCTGAAGCGGATCAGAATCAGATCAATTTTTCAAAGCTTTTGTCGGACCA
AGAAATTATTTATGTACCTAAAGTTGGAGAGGAAATCCCTAGATTAAAAGAAGCAACTGTTGAATCAGATGGATCAATTG
GAGGGGAATCTCAGGAAAAGATCAACATCAACACAGCGGATGCGACAGAATTGCAGAAATTATCAGGAATTGGTGAGAAA
AAAGCGACGGATATAATCAAATATCGAGAGGAAAATGGCAGCTTTAACGCGATCGATGATCTAACAAAAGTATCCGGCAT
TGGTGAGAAAACGCTTGAAAATTTAAAGGACTCAATTACAATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(137-200)

SLBB domain (SLBB)

(58-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

48.325

100

0.502
  comEA Streptococcus thermophilus LMD-9

37.826

100

0.433
  comEA Bacillus subtilis subsp. subtilis str. 168

40

100

0.408
  comEA Latilactobacillus sakei subsp. sakei 23K

34.632

100

0.398
  comEA Staphylococcus aureus MW2

33.636

100

0.368
  comEA Staphylococcus aureus N315

33.182

100

0.363