Detailed information NTG0596894

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K4120_RS12340
• Genome accession: NZ_CP081488
• Coordinates: 2550141..2550755 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LZN01
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545141..2555755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4120_RS12310 (K4120_12310)	rpsT	2545444..2545710 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
K4120_RS12315 (K4120_12315)	holA	2545727..2546768 (-)	1042	Protein_2396	DNA polymerase III subunit delta	-
K4120_RS12320 (K4120_12320)	-	2546803..2546959 (-)	157	Protein_2397	hypothetical protein	-
K4120_RS12325 (K4120_12325)	-	2547000..2547134 (+)	135	WP_003152870.1	YqzM family protein	-
K4120_RS12330 (K4120_12330)	comEC	2547153..2549504 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K4120_RS12335 (K4120_12335)	-	2549505..2550074 (-)	570	WP_003152868.1	ComE operon protein 2	-
K4120_RS12340 (K4120_12340)	comEA	2550141..2550755 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
K4120_RS12345 (K4120_12345)	comER	2550814..2551635 (+)	822	WP_032866690.1	late competence protein ComER	-
K4120_RS12350 (K4120_12350)	-	2551704..2552441 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
K4120_RS12355 (K4120_12355)	rsfS	2552438..2552794 (-)	357	WP_007408260.1	ribosome silencing factor	-
K4120_RS12360 (K4120_12360)	yqeK	2552812..2553372 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K4120_RS12365 (K4120_12365)	-	2553362..2553931 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
K4120_RS12370 (K4120_12370)	yhbY	2553942..2554232 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
K4120_RS12375 (K4120_12375)	aroE	2554226..2555068 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=596894 K4120_RS12340 WP_032866693.1 2550141..2550755(-) (comEA) [Bacillus velezensis strain LZN01]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=596894 K4120_RS12340 WP_032866693.1 2550141..2550755(-) (comEA) [Bacillus velezensis strain LZN01]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363