Detailed information NTG0595640

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K3169_RS14315
• Genome accession: NZ_CP081201
• Coordinates: 3162747..3163916 (+)
• Length: 389 a.a.
• NCBI ID: WP_122704761.1
• Uniprot ID: -
• Organism: Pseudomonas phytophila strain ICMP 23753
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3157747..3168916

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K3169_RS14285 (K3169_14170)	-	3157956..3158285 (-)	330	WP_231677574.1	DUF6388 family protein	-
K3169_RS14290 (K3169_14175)	-	3158595..3158984 (+)	390	WP_027899441.1	RidA family protein	-
K3169_RS14295 (K3169_14180)	-	3159137..3159901 (+)	765	WP_263272079.1	IclR family transcriptional regulator	-
K3169_RS14300 (K3169_14185)	-	3159918..3161123 (+)	1206	WP_027899439.1	amino acid deaminase	-
K3169_RS14305 (K3169_14190)	-	3161141..3162085 (+)	945	WP_231677577.1	sugar kinase	-
K3169_RS14310 (K3169_14195)	-	3162234..3162509 (-)	276	WP_017133756.1	peptidylprolyl isomerase	-
K3169_RS14315 (K3169_14200)	pilU	3162747..3163916 (+)	1170	WP_122704761.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K3169_RS14320 (K3169_14205)	-	3165234..3166004 (-)	771	WP_122397072.1	3-hydroxybutyrate dehydrogenase	-
K3169_RS14325 (K3169_14210)	-	3166015..3167406 (-)	1392	WP_263272081.1	GntP family permease	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42706.75 Da
• Isoelectric Point: 6.4353

>NTDB_id=595640 K3169_RS14315 WP_122704761.1 3162747..3163916(+) (pilU) [Pseudomonas phytophila strain ICMP 23753]
MDFPALLKTLASQDGSDLYLSTGAPPCAKFNGVLKPLGTESLKPGQVALVADGLMDAEQKRDFHRDLEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLHLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDHRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLCLSTLHANNANQALDRI
INFFPEERRQQLLQDLGNNLKAFVSQRLVKTLDGNRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGNLGMQTFDGAL
FELAAAGVISEEEALKYADSQNNLRLRLKLHGEGVTANPTAAPSAPVGTSSVNVSDWGLVDERDEGGQN

Nucleotide

• Length: 1170 bp

>NTDB_id=595640 K3169_RS14315 WP_122704761.1 3162747..3163916(+) (pilU) [Pseudomonas phytophila strain ICMP 23753]
ATGGATTTCCCGGCGTTGTTGAAGACTCTGGCCAGTCAGGATGGATCGGACCTTTATCTTTCTACTGGGGCACCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGTTGGGCACTGAGTCCCTCAAGCCTGGTCAGGTGGCTCTGGTTGCCGACGGGT
TGATGGATGCCGAGCAGAAGCGGGATTTCCATCGAGATCTTGAGATGAACCTCGCGGTGTCCATGGCAGGGATCGGGCGT
TTCCGTATCAATATCTTCATGCAGCGCAACGAAGTATCGATCGTGGCGCGAAACATCAAGCTGGATATCCCGCGCTTCGA
AGACCTGCACCTTCCTCCGGTGCTGCTCGACGTGATCATGGAGAAGCATGGCCTGGTGCTGTTTGTGGGCGCGACCGGTT
CAGGCAAATCCACCTCACTGGCGGCGCTGATCGATCACCGCAACCGCAATGCCAGCGGGCACATCATTACCATTGAAGAC
CCGGTGGAATTCATTCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGGGTTGATACCCGCAGTTTTCGTGC
TGCATTGAAGAACACCCTGCGCCAGGCACCGGATGTCATCCTGATCGGTGAAATCCGCGATCGGGAAACCATGGAACATG
CGCTGGCCTTCGCCGATACCGGACACTTGTGCCTTTCCACACTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATT
ATCAACTTCTTCCCGGAAGAACGACGCCAGCAACTGCTGCAGGACTTGGGCAACAACCTCAAAGCGTTCGTTTCCCAGCG
TCTGGTCAAGACTCTGGATGGCAACCGCAGGGCGGCCGTGGAGGTCATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAGTTGACTGAACTCAAAGGCATCATGGAAAAGTCCGGAAATCTCGGCATGCAGACCTTCGACGGTGCACTG
TTCGAGCTGGCAGCAGCCGGTGTGATCAGCGAAGAGGAAGCGCTCAAATACGCCGATTCCCAGAACAACCTCCGCCTGCG
CCTCAAGCTGCATGGTGAAGGCGTGACGGCCAACCCCACCGCCGCGCCGAGCGCTCCTGTCGGTACCTCCAGCGTGAACG
TGTCGGATTGGGGGTTGGTGGATGAGCGCGATGAGGGTGGGCAGAACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.627	91.003	0.524
pilU	Acinetobacter baylyi ADP1	52.809	91.517	0.483
pilU	Vibrio cholerae strain A1552	50.857	89.974	0.458
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.153	97.172	0.38
pilT	Pseudomonas aeruginosa PAK	42.029	88.689	0.373
pilT	Pseudomonas stutzeri DSM 10701	41.739	88.689	0.37