Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSYCG_RS00345 Genome accession   NC_021661
Coordinates   79063..80775 (-) Length   570 a.a.
NCBI ID   WP_020442462.1    Uniprot ID   -
Organism   Psychrobacter sp. G     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74063..85775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYCG_RS00320 (PSYCG_00505) - 74628..74963 (+) 336 WP_020442457.1 pyrimidine/purine nucleoside phosphorylase -
  PSYCG_RS00325 (PSYCG_00510) rlmB 75168..75938 (+) 771 WP_020442458.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PSYCG_RS00330 (PSYCG_00515) coaE 75901..76593 (-) 693 WP_020442459.1 dephospho-CoA kinase -
  PSYCG_RS00335 (PSYCG_00520) pilD 76677..77576 (-) 900 WP_020442460.1 prepilin peptidase Machinery gene
  PSYCG_RS00340 (PSYCG_00525) pilC 77602..78825 (-) 1224 WP_020442461.1 type II secretion system F family protein Machinery gene
  PSYCG_RS00345 (PSYCG_00530) pilB 79063..80775 (-) 1713 WP_020442462.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYCG_RS00350 (PSYCG_00535) tpiA 81173..81982 (+) 810 WP_020442463.1 triose-phosphate isomerase -
  PSYCG_RS00355 (PSYCG_00540) secG 82211..82519 (+) 309 WP_011512446.1 preprotein translocase subunit SecG -
  PSYCG_RS00370 (PSYCG_00560) rimP 83423..83923 (+) 501 WP_011512447.1 ribosome maturation factor RimP -
  PSYCG_RS00375 (PSYCG_00565) nusA 84057..85541 (+) 1485 WP_011512448.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63200.53 Da        Isoelectric Point: 5.3322

>NTDB_id=59431 PSYCG_RS00345 WP_020442462.1 79063..80775(-) (pilB) [Psychrobacter sp. G]
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDALSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDL
HIGFDENQEEDDGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKP
QGMLLITGPTGSGKTVSLYTGINLLNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGF
TDTDLDNTDNVIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTS
IQEMMRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=59431 PSYCG_RS00345 WP_020442462.1 79063..80775(-) (pilB) [Psychrobacter sp. G]
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCACAGCAGCTGATTAGCGAAGGTATCGTAAGCGAAGCGCATATGAA
AGCGGCGCAGACTGATTCGCAGCAGCAGCAAGTAGGACTGGTCTCTTATCTGGTAGAGCATAAGCTCGCCAATGCCTATC
AATTGGCTCAAATGCTATCGCAAGCATTCGGCGATCCTTTATTTGATCTCGATGCGCTCAGCTCAGAGGTCATCCCAAAA
GACTTGGTCGACGAAAAAATCGTCCGTAAGTTTAATGCCTTACCAATGTTTAAGCGTGGGCAACGCTTATTTGTGGCACT
GAGCGATCCGACCCGTATCGATGCGATTGATGCGATTGCCTTTAACTCTCGCTTGTCAATTGAAACCGTTGTAGTCGAAG
AAGATAAGCTTAAAAGGCGTATTGAAAGCTTCTATGCTGATAGTATGCAAAATTTTGATAGCTTTTCTAATAGTGATTTG
CATATTGGCTTTGATGAGAATCAAGAAGAAGATGACGGTGAGACCAAAATCTCTGATAGCGTCGAGGAAGCACCGGTCGT
CAAGTTTGTTAATAAAATGCTAGTAGATGCCATTCGTATGGGTGCGTCCGACTTACATTTTGAGCCCTATGAAAAAAGCT
ATCGAGTGCGCTTTCGAGTCGACGGTGTGATGCAAAAAATGGCCAACCCGCCCGTACAACTCGCGGGGAAAATTGCTGCG
CGTTTAAAAGTAATGTCACAAATGGATATCTCAGAGCGCCGTGTCCCTCAAGATGGACGTATTAAGCTAAAGATATCTAA
AAATAAGGCCATCGATTTTCGGGTAAGTTGCCTACCTACCTTGTTTGGCGAAAAACTGGTTTTGCGTATTCTAGATCCCT
CATCAGCCATGCTTGGTATTGAAGCGTTGGGCTATGAGCCTGACCAACAAGATATGTTTTTGGAGGCGTTACATAAACCG
CAAGGCATGCTCCTAATTACTGGTCCCACTGGTTCTGGTAAAACCGTCTCACTCTATACTGGTATTAACCTCCTGAATAC
TGGTTCGACCAATATCTCGACTGCCGAAGATCCTGTCGAAATTAACCTTGAAGGTATTAATCAGGTCAACGTCAACCCAA
AAGTGGGGCTGACTTTCTCCACCGCCCTTAAGTCTTTCTTACGCCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGT
GATCTTGAAACTGCTGAGATTGCGATTAAAGCAGCACAGACAGGGCACATGGTACTTTCAACCTTACATACCAATTCAGC
GCCTGAAACGTTAACGCGATTACGCAATATGGGTGTCGCTTCTTTTAATATTGCCACTTCCGTGAACTTGGTCATTGCCC
AACGATTGGCACGGCGCTTATGTAAAAACTGTAAAAAACCTATCAATATTCCGCGTCAAAGTCTGCTTGAGCTTGGTTTT
ACAGATACAGATTTGGACAATACAGACAATGTCATCTACGAGCCAGTCGGTTGTAATGAGTGCCGTGAAGGCTACAAAGG
ACGTGTGGGCATTTATGAGGTGATGAAAGTCACTCCAGATATTTCACGTATTATCATGGAAGATGGTAACGCGATAGACA
TCAAAGATGCGGCGCTAAAAAACGGTTTTAGAGACTTACGTCGCTCTGGTGTTTTAAAGGTTTTACAAGGTGTCACCAGT
ATTCAAGAAATGATGCGCGTGACTTCTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.719

100

0.677
  pilB Acinetobacter baylyi ADP1

63.732

99.649

0.635
  pilB Legionella pneumophila strain ERS1305867

51.127

100

0.518
  pilF Neisseria gonorrhoeae MS11

48.592

99.649

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

51.048

92.105

0.47
  pilB Vibrio cholerae strain A1552

50.471

93.158

0.47
  pilB Vibrio campbellii strain DS40M4

49.064

93.684

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.455

100

0.382

Multiple sequence alignment