Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K1516_RS17160 Genome accession   NZ_CP080573
Coordinates   3699111..3700841 (-) Length   576 a.a.
NCBI ID   WP_248858195.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain FZD2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3688260..3724766 3699111..3700841 within 0


Gene organization within MGE regions


Location: 3688260..3724766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1516_RS17115 (K1516_17120) - 3688260..3688934 (+) 675 WP_248858193.1 hypothetical protein -
  K1516_RS17120 (K1516_17125) - 3688928..3689941 (+) 1014 WP_248858194.1 hypothetical protein -
  K1516_RS17125 (K1516_17130) - 3689935..3690951 (+) 1017 WP_110713347.1 TraB/GumN family protein -
  K1516_RS17130 (K1516_17135) - 3691270..3691539 (+) 270 WP_110713349.1 hypothetical protein -
  K1516_RS17135 (K1516_17140) - 3691591..3693348 (+) 1758 WP_164162035.1 ABC transporter transmembrane domain-containing protein -
  K1516_RS17140 (K1516_17145) sucD 3693466..3694341 (-) 876 WP_005410793.1 succinate--CoA ligase subunit alpha -
  K1516_RS17145 (K1516_17150) sucC 3694362..3695531 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  K1516_RS17150 (K1516_17155) - 3695937..3697550 (+) 1614 WP_164162034.1 ATP-binding protein -
  K1516_RS17155 (K1516_17160) pilR 3697611..3698984 (+) 1374 WP_110713353.1 sigma-54 dependent transcriptional regulator Regulator
  K1516_RS17160 (K1516_17165) pilB 3699111..3700841 (-) 1731 WP_248858195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K1516_RS17165 (K1516_17170) - 3701057..3701464 (-) 408 WP_180854230.1 pilin -
  K1516_RS17175 (K1516_17175) comP 3701590..3702039 (-) 450 WP_248858196.1 pilin Machinery gene
  K1516_RS17180 (K1516_17180) pilC 3702394..3703653 (+) 1260 WP_153855677.1 type II secretion system F family protein Machinery gene
  K1516_RS17185 (K1516_17185) - 3703662..3704525 (+) 864 WP_010481503.1 A24 family peptidase -
  K1516_RS17190 (K1516_17190) coaE 3704537..3705148 (+) 612 WP_248858197.1 dephospho-CoA kinase -
  K1516_RS17195 (K1516_17195) - 3705278..3705664 (-) 387 WP_248858198.1 hypothetical protein -
  K1516_RS17200 (K1516_17200) - 3706089..3708254 (-) 2166 WP_248858199.1 DUF2235 domain-containing protein -
  K1516_RS17205 (K1516_17205) - 3708251..3708877 (-) 627 WP_248858200.1 DUF3304 domain-containing protein -
  K1516_RS17210 (K1516_17210) - 3708878..3709843 (-) 966 WP_248858202.1 DUF2235 domain-containing protein -
  K1516_RS17215 (K1516_17215) - 3710020..3710646 (-) 627 WP_164162028.1 DUF3304 domain-containing protein -
  K1516_RS17220 (K1516_17220) - 3710647..3711408 (-) 762 WP_343236006.1 DUF2235 domain-containing protein -
  K1516_RS22160 - 3711385..3711675 (+) 291 WP_343236007.1 hypothetical protein -
  K1516_RS17225 (K1516_17230) - 3712039..3712374 (-) 336 WP_248858205.1 hypothetical protein -
  K1516_RS17230 (K1516_17235) - 3712413..3713417 (-) 1005 WP_248858206.1 DUF2235 domain-containing protein -
  K1516_RS17235 (K1516_17240) - 3713591..3714208 (-) 618 WP_248858207.1 DUF3304 domain-containing protein -
  K1516_RS17240 (K1516_17245) - 3714375..3715190 (-) 816 WP_248858208.1 DUF4123 domain-containing protein -
  K1516_RS17245 (K1516_17250) tssI 3715183..3717792 (-) 2610 WP_248858209.1 type VI secretion system Vgr family protein -
  K1516_RS17250 (K1516_17255) - 3718015..3718431 (-) 417 WP_248858210.1 hypothetical protein -
  K1516_RS17255 (K1516_17260) - 3718702..3720072 (-) 1371 WP_110713374.1 HAMP domain-containing sensor histidine kinase -
  K1516_RS17260 (K1516_17265) - 3720035..3720712 (-) 678 WP_005410804.1 response regulator transcription factor -
  K1516_RS17265 (K1516_17270) - 3720725..3721210 (-) 486 WP_110713376.1 hypothetical protein -
  K1516_RS17270 (K1516_17275) rimK 3721496..3722374 (-) 879 WP_110713377.1 30S ribosomal protein S6--L-glutamate ligase -
  K1516_RS17275 (K1516_17280) - 3722504..3722941 (+) 438 WP_180846449.1 thioesterase family protein -
  K1516_RS17280 (K1516_17285) - 3722942..3724096 (-) 1155 WP_248858211.1 hypothetical protein -
  K1516_RS17285 (K1516_17290) - 3724242..3724766 (+) 525 WP_248858212.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62172.18 Da        Isoelectric Point: 6.0452

>NTDB_id=594158 K1516_RS17160 WP_248858195.1 3699111..3700841(-) (pilB) [Stenotrophomonas maltophilia strain FZD2]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPIIVDEDQIRRTLEQWHASHDTLGDALGGD
DEGMGNLEVAAGDEDLGAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKKRSELPAH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=594158 K1516_RS17160 WP_248858195.1 3699111..3700841(-) (pilB) [Stenotrophomonas maltophilia strain FZD2]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGATGA
GGCTTCCGCCCGCGATGCCATGGCCAAGGCAGCCGCCGCCCGGCAGCCGCTGCCCACCTGGTTCGCGCAGAACAAGGTGG
TCGGCGCTGCGCAGCTGGCAGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTATCCACGTTCGACGCCAGC
CAGAACGCCATGAGCCTGGTCAGCGAGGAGCTGCTGCGGAAACACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGATGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCATCG
TCGATGAGGACCAGATCCGCCGGACGCTGGAGCAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGCGGCGAC
GATGAGGGCATGGGAAACCTGGAAGTCGCGGCTGGCGACGAGGACCTGGGGGCCGGTGGCGACTCCGGCATCGATGCCAA
GGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGCAAGGGCGCTTCGGATATCCACT
TCGAACCCTACGAAGACGATTATCGTGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAA
TTGAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGGTCG
CATCAAGCTGAACCTGTCCAAGTCCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGAAGCCATCCATAAGCCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCAACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCATACCAACGATGCGCCGCAGACCATCGCGCGACTGATGAACATGGGCATTGCGCCCTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCTTGTGTGGCAACTGCAAGAAACGCTCGGAGCTTCCCGCGCAC
GCGTTGCTGGCCGAGGGCTTCACCCAGGCGCAGTTGGACGCAGGCATTGAGCTGTACGAGGCGGTGGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGCCTTTACCAGGTGATGCCGATGACCGATGAGATCGCCACCATCGTGCTGGCTG
GCGGTAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGATCTGCGCCAATCCGCGCTGAAGAAGGCG
GCGGCGGGCATCACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.639

100

0.552
  pilB Acinetobacter baylyi ADP1

54.11

100

0.549
  pilB Legionella pneumophila strain ERS1305867

52.548

98.785

0.519
  pilF Neisseria gonorrhoeae MS11

49.472

98.611

0.488
  pilB Vibrio cholerae strain A1552

48.421

98.958

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.306

0.451
  pilB Vibrio campbellii strain DS40M4

44.991

98.785

0.444