Detailed information NTG0586499

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EVX74_RS02130
• Genome accession: NZ_CP078045
• Coordinates: 488082..489140 (+)
• Length: 352 a.a.
• NCBI ID: WP_129717185.1
• Uniprot ID: A0AAJ4P523
• Organism: Acinetobacter lwoffii strain AL_065
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 483082..494140

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EVX74_RS02115 (EVX74_002105)	mraY	483363..484481 (+)	1119	WP_129717180.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
EVX74_RS02120 (EVX74_002110)	-	484536..485339 (+)	804	WP_129717181.1	putative RNA methyltransferase	-
EVX74_RS02125 (EVX74_002115)	ponA	485391..487916 (-)	2526	WP_129717183.1	penicillin-binding protein PBP1a	-
EVX74_RS02130 (EVX74_002120)	pilM	488082..489140 (+)	1059	WP_129717185.1	pilus assembly protein PilM	Machinery gene
EVX74_RS02135 (EVX74_002125)	-	489140..489779 (+)	640	Protein_412	PilN domain-containing protein	-
EVX74_RS02140 (EVX74_002130)	-	489776..490498 (+)	723	Protein_413	type 4a pilus biogenesis protein PilO	-
EVX74_RS02145 (EVX74_002135)	pilP	490498..491025 (+)	528	WP_004732447.1	pilus assembly protein PilP	Machinery gene
EVX74_RS02150 (EVX74_002140)	pilQ	491044..493209 (+)	2166	WP_004732444.1	type IV pilus secretin PilQ family protein	Machinery gene
EVX74_RS02155 (EVX74_002145)	aroK	493246..493782 (+)	537	WP_004732442.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38996.53 Da
• Isoelectric Point: 4.6329

>NTDB_id=586499 EVX74_RS02130 WP_129717185.1 488082..489140(+) (pilM) [Acinetobacter lwoffii strain AL_065]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSGRYWVESYALVPLSENSVVEKNILNPEAVADALGRAINLGNTQSNQAAF
AIPTSMVITKIIEMDADMSDDKREVQIREDAEQYIPFPLDEASLDFEVLSDRLTNPNRVNVLLVATRIENVEARSEVLEI
SGLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=586499 EVX74_RS02130 WP_129717185.1 488082..489140(+) (pilM) [Acinetobacter lwoffii strain AL_065]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAGGTTTTAGAACT
TTCTGTAAAAAGTGGCCGTTACTGGGTAGAGAGCTATGCTTTGGTCCCATTATCGGAAAACAGTGTTGTTGAAAAAAACA
TCTTAAACCCAGAAGCAGTCGCTGATGCCCTTGGGCGTGCAATTAATTTGGGCAATACCCAGTCGAATCAGGCCGCTTTT
GCGATTCCAACTTCAATGGTCATCACCAAAATCATTGAGATGGATGCAGATATGTCGGATGACAAACGTGAGGTTCAGAT
TCGTGAAGATGCGGAACAATATATTCCTTTCCCACTCGATGAAGCGAGTCTGGACTTTGAAGTTCTGTCAGACCGTCTTA
CTAATCCAAACCGGGTCAATGTGCTATTGGTCGCTACCCGAATTGAAAATGTCGAAGCACGTTCTGAAGTTTTGGAAATT
TCTGGTCTAACGCCTAAGATTGCAGATGTAGAAAGTTTTGCGCTGGAAAATGCCTTCAAAGTATTTTCGGATACTTTGCC
GATGGGGGTCAATACGGTTGGTATCTTAGATATTGGTCATAGTATGACGACCTTGTCGGTGATGCAGAACAACAAGATTA
TTTATACCCGTGAGCAGGTGTTTGGCGGCAAGCAATTGACTCAGGAAATTCAGAATCGCTATGGATTATCCTTTGAAGAA
GCTGGACGTGCTAAAAAAACCCGTGCTTTACCGGATGATTATGATATTGAAGTGCTAGAGCCATTCCTGGATGCCGTGGT
ACAACAGGCAGCCCGTTCATTGCAATTCTTCTTTTCATCTTCTCAGTTTAATGAGATTGACCATATTTTACTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGCGGTTTGGC
ATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	84.659	100	0.847
comM	Acinetobacter nosocomialis M2	84.659	100	0.847
comM	Acinetobacter baylyi ADP1	79.83	100	0.798
pilM	Legionella pneumophila strain ERS1305867	39.266	100	0.395