Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EVX74_RS02150 Genome accession   NZ_CP078045
Coordinates   491044..493209 (+) Length   721 a.a.
NCBI ID   WP_004732444.1    Uniprot ID   A0AAJ4P5Q4
Organism   Acinetobacter lwoffii strain AL_065     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486044..498209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVX74_RS02130 (EVX74_002120) pilM 488082..489140 (+) 1059 WP_129717185.1 pilus assembly protein PilM Machinery gene
  EVX74_RS02135 (EVX74_002125) - 489140..489779 (+) 640 Protein_412 PilN domain-containing protein -
  EVX74_RS02140 (EVX74_002130) - 489776..490498 (+) 723 Protein_413 type 4a pilus biogenesis protein PilO -
  EVX74_RS02145 (EVX74_002135) pilP 490498..491025 (+) 528 WP_004732447.1 pilus assembly protein PilP Machinery gene
  EVX74_RS02150 (EVX74_002140) pilQ 491044..493209 (+) 2166 WP_004732444.1 type IV pilus secretin PilQ family protein Machinery gene
  EVX74_RS02155 (EVX74_002145) aroK 493246..493782 (+) 537 WP_004732442.1 shikimate kinase AroK -
  EVX74_RS02160 (EVX74_002150) aroB 493811..494890 (+) 1080 WP_004732440.1 3-dehydroquinate synthase -
  EVX74_RS02165 (EVX74_002155) - 494901..495779 (+) 879 WP_004732438.1 hypothetical protein -

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 78131.38 Da        Isoelectric Point: 4.9982

>NTDB_id=586501 EVX74_RS02150 WP_004732444.1 491044..493209(+) (pilQ) [Acinetobacter lwoffii strain AL_065]
MNKSIKEFIFGDANTMNRVFRQFSMGAVAIAVMQAASAQVAVTNVVPMQLPGQGTEIRVMFNGLPPQPQAYQLESPSRLI
LDFDKAQQNLKQSNIPVATREASSVEVTSDAQRARLTVNLTDAGAFTTRVEGNTFILKINSATPVAIAQPVQQQSAQGIS
NIGFQRGTQGEGQVVIDLLGNNTPVDVQQQGTKVIVRTLGSKIPAHLTRRLNVTDFATPVSSVDAVNQNGSGVITIQSAE
SYEYMAYQTDNKLTISLKRPEAKSPLRPKTVAYSGKKISLDFQDIEVRRVLQLLADFTDINMVAADSVQGNITLRLKEVP
WDQALDIVLKTKNLDKRRNGNVIWIAPVAELIKAEEEEAKAIKQSVALAPIQTEYMQLSYAKAADIEKLITQNKGASSSG
GSSSTNNNDGGESLLSSRGSVSIDARTNTIIVNDTQLFIDKIRNMVDLLDVQVKQVMVEARIVRATTDFTKEMGVKWGIL
SQGITNNDSLLVGGSDTTLWDLRDPQESDLGGYTYEIQRPQNLNVDLGVTSPGASRIAFGLISLSDFMLDLELSALQADG
YGEVISTPKVLTADKQKATVASGSQIPYQSAEGGGANAVSTTEFIDATLSLDVTPSITPDGKVQMELNITSDSPGNPTPT
GQLTINKNQINTNVLVDNGETVVLGGIFEQETRNAQTKVPFLGDIPYVGRLFRKDLKSDNKRELLIFVTPRIVNDSVSRN
H

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=586501 EVX74_RS02150 WP_004732444.1 491044..493209(+) (pilQ) [Acinetobacter lwoffii strain AL_065]
ATGAATAAGAGTATTAAAGAATTTATTTTTGGGGATGCTAATACAATGAATCGTGTGTTTCGTCAGTTTTCAATGGGTGC
TGTTGCAATTGCGGTGATGCAAGCAGCCAGCGCACAAGTGGCCGTAACGAATGTGGTACCTATGCAGCTTCCTGGTCAAG
GAACTGAAATTCGGGTGATGTTTAATGGTTTACCACCTCAACCACAAGCCTATCAATTAGAATCACCGTCACGTTTGATT
CTGGACTTTGACAAGGCCCAACAGAATTTAAAACAAAGCAACATTCCGGTCGCAACGCGTGAAGCCAGTTCAGTGGAGGT
CACTTCGGATGCACAGCGTGCCCGTTTGACGGTTAATCTGACAGATGCAGGTGCCTTTACCACTCGAGTAGAAGGCAATA
CTTTTATTCTTAAAATTAACTCTGCAACTCCTGTAGCCATAGCTCAACCTGTGCAGCAACAGTCAGCTCAGGGTATTAGC
AATATTGGTTTCCAGCGCGGTACGCAAGGTGAAGGCCAGGTCGTTATTGATCTATTAGGAAATAATACTCCTGTTGATGT
ACAGCAGCAGGGTACCAAGGTTATTGTGCGCACTTTAGGTTCCAAGATTCCGGCTCATTTGACCCGCCGTCTTAATGTGA
CAGATTTCGCGACTCCGGTATCAAGTGTGGATGCTGTGAATCAAAATGGTTCAGGAGTCATTACGATTCAGTCTGCGGAA
AGTTATGAATATATGGCTTACCAAACAGATAATAAGCTGACCATCAGCTTGAAACGTCCGGAAGCCAAGAGTCCATTGCG
TCCTAAAACTGTCGCTTATAGCGGTAAGAAAATTTCGCTAGATTTCCAAGATATTGAAGTGCGCCGCGTGTTGCAGTTAT
TGGCAGATTTTACCGATATCAATATGGTGGCTGCAGATAGTGTGCAAGGTAATATTACCTTGCGCTTGAAAGAGGTACCT
TGGGATCAGGCGCTGGATATTGTGCTGAAAACCAAAAACCTGGATAAACGCCGTAATGGCAATGTGATCTGGATCGCACC
AGTTGCAGAGCTGATTAAGGCTGAGGAGGAAGAAGCTAAGGCTATTAAGCAGAGTGTTGCACTTGCCCCTATCCAAACTG
AATATATGCAGCTGAGTTATGCCAAAGCTGCTGATATCGAAAAACTGATTACGCAGAATAAAGGGGCCTCTAGCTCAGGC
GGCTCGAGTAGTACGAATAATAATGATGGTGGAGAAAGCTTATTGAGTTCACGTGGCTCTGTGTCTATTGATGCACGAAC
CAATACCATTATTGTGAATGATACTCAGCTCTTTATTGATAAAATCCGGAATATGGTAGACTTGCTCGATGTACAGGTCA
AGCAGGTGATGGTCGAAGCACGTATTGTGCGAGCTACCACTGACTTCACCAAAGAAATGGGAGTGAAGTGGGGGATTCTA
TCTCAAGGGATTACCAACAATGATAGCCTTCTGGTCGGTGGTAGTGACACGACGTTATGGGATCTCAGAGATCCCCAAGA
AAGTGATTTAGGTGGATATACTTATGAGATTCAACGTCCACAAAACCTTAATGTAGACTTGGGTGTAACCAGTCCGGGTG
CAAGTCGTATTGCTTTTGGTCTAATCAGTCTTTCTGATTTTATGCTGGATCTTGAGCTTTCTGCACTTCAGGCAGATGGC
TATGGTGAAGTGATTTCTACACCTAAAGTGCTGACAGCGGACAAGCAAAAAGCGACTGTTGCCTCTGGTTCACAAATTCC
GTACCAGTCAGCAGAAGGTGGTGGTGCGAATGCAGTGTCCACTACTGAGTTTATTGATGCTACCTTAAGTCTGGATGTCA
CCCCAAGCATTACGCCAGATGGCAAAGTACAGATGGAACTTAATATTACCAGTGACTCACCGGGTAATCCGACACCGACT
GGACAGCTCACTATTAATAAAAACCAGATCAATACCAATGTCTTGGTCGATAATGGTGAAACAGTCGTCCTAGGTGGTAT
TTTTGAGCAGGAAACCCGTAATGCGCAAACCAAGGTACCATTCTTGGGGGATATCCCTTATGTAGGACGTTTATTCCGTA
AAGATTTAAAGTCGGATAATAAACGTGAGCTACTGATTTTCGTTACACCTCGAATTGTTAATGACAGTGTTTCAAGAAAT
CATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-347)

AMIN domain (AMIN)

(55-134)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-455)

Bacterial type II and III secretion system protein (Secretin)

(555-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

73.895

100

0.742
  pilQ Acinetobacter baumannii strain A118

73.757

100

0.741
  comQ Acinetobacter baylyi ADP1

71.229

99.307

0.707
  pilQ Pseudomonas aeruginosa PAK

41.954

96.533

0.405
  pilQ Legionella pneumophila strain Lp02

37.006

98.197

0.363