Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KQP93_RS14180 Genome accession   NZ_CP077770
Coordinates   3052362..3053495 (-) Length   377 a.a.
NCBI ID   WP_217874942.1    Uniprot ID   -
Organism   Pseudoalteromonas shioyasakiensis strain SDCH90     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3047362..3058495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQP93_RS14150 - 3048154..3048615 (+) 462 WP_217874937.1 hypothetical protein -
  KQP93_RS14155 rsmE 3048685..3049419 (+) 735 WP_217874938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KQP93_RS14160 gshB 3049488..3050441 (+) 954 WP_217874939.1 glutathione synthase -
  KQP93_RS14165 - 3050466..3051023 (+) 558 WP_217874940.1 YqgE/AlgH family protein -
  KQP93_RS14170 ruvX 3051043..3051492 (+) 450 WP_217874941.1 Holliday junction resolvase RuvX -
  KQP93_RS14175 - 3051489..3052361 (-) 873 WP_254907678.1 hypothetical protein -
  KQP93_RS14180 pilU 3052362..3053495 (-) 1134 WP_217874942.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KQP93_RS14185 pilT 3053510..3054550 (-) 1041 WP_217874943.1 type IV pilus twitching motility protein PilT Machinery gene
  KQP93_RS14190 - 3054553..3055266 (+) 714 WP_217874944.1 YggS family pyridoxal phosphate-dependent enzyme -
  KQP93_RS14195 proC 3055284..3056105 (+) 822 WP_217874945.1 pyrroline-5-carboxylate reductase -
  KQP93_RS14200 - 3056129..3056662 (+) 534 WP_054562743.1 YggT family protein -
  KQP93_RS14205 - 3056721..3057161 (+) 441 WP_217874946.1 DUF4426 domain-containing protein -
  KQP93_RS14210 - 3057169..3057768 (+) 600 WP_217874947.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41896.91 Da        Isoelectric Point: 6.5069

>NTDB_id=582883 KQP93_RS14180 WP_217874942.1 3052362..3053495(-) (pilU) [Pseudoalteromonas shioyasakiensis strain SDCH90]
MSLPLNHFLQVMIDKKASDLFVSSQLPVSAKINGELVHLTDERLSDDASLALVESAMNDKQRKEFHDTKECNFAIATDEG
RFRVSAFWQRDRAGMVIRRIVTQIPDVNELGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGEGRVAAIEILLNSPMVAELIKRGDIGSIKETMAKSKEMGMQTFDQ
ALFELYKQRRINYADALHHADSPNDLRLMIKLQNNEQSGAGFLQGVTIDGLDGKSDK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=582883 KQP93_RS14180 WP_217874942.1 3052362..3053495(-) (pilU) [Pseudoalteromonas shioyasakiensis strain SDCH90]
ATGTCTTTACCTTTAAATCATTTTTTACAAGTTATGATCGATAAAAAAGCGTCTGACTTGTTTGTTTCTAGCCAGCTGCC
AGTCAGTGCAAAAATCAACGGTGAGCTAGTGCACCTTACCGACGAAAGGCTTTCGGATGATGCTTCCCTTGCGTTAGTTG
AGTCGGCTATGAATGATAAGCAACGCAAAGAGTTTCATGACACTAAAGAGTGTAACTTTGCGATTGCAACCGATGAAGGT
CGTTTCCGTGTTTCTGCTTTTTGGCAGCGTGACAGAGCCGGTATGGTTATTCGCCGTATCGTGACGCAAATCCCAGATGT
AAACGAATTAGGTTTACCATCAACGTTAACCGATGTGATCATGGCAAAACGTGGTTTAGTACTATTTGTAGGTGGTACAG
GTACCGGTAAATCGACCTCGCTCGCTGCGCTTTTAGGTTATCGCAACCGCAATCAACGTGGTCATATTCTTACCATTGAA
GATCCTATCGAATTTGTTCATGAACATCGTAAAAGTATTATCACTCAACGTGAAGTTGGTTTAGATACTGAAAGCTTTGA
ATCTGCGCTGAAAAGCTCGTTACGTCAAGCACCTGATGTGATCTTAATTGGTGAGATCCGTTCACAAGAAACCATGGAAT
ACGCACTCAGCTTCGCTGAAACTGGTCACTTGTGTGTGGCGACACTGCATGCGAATAACGCTAACCAAGCCATTGACCGT
ATCATGCACTTAGTGCCGAAAGAAAAGCACGATAAGTTAAAGTACGACCTAGCACTTAACCTTCGTGCCATTGTCGCGCA
GCAGTTGGTGCCAACTGCCGATGGGGAAGGGCGTGTTGCTGCTATCGAAATTTTGCTTAATTCACCTATGGTGGCAGAAC
TCATCAAGCGTGGTGACATTGGCTCTATCAAAGAAACCATGGCTAAATCGAAAGAAATGGGCATGCAGACCTTTGACCAA
GCGTTATTTGAGCTTTATAAACAGCGCCGTATTAACTACGCTGATGCGCTACACCATGCTGACTCGCCAAATGACTTGCG
CTTAATGATTAAGCTGCAAAACAATGAACAAAGTGGTGCGGGCTTCTTACAAGGCGTGACTATCGATGGCCTTGATGGAA
AGAGCGACAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.038

97.347

0.565
  pilU Pseudomonas stutzeri DSM 10701

59.249

91.777

0.544
  pilU Acinetobacter baylyi ADP1

58.239

93.369

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.134

94.96

0.419
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366