Detailed information NTG0580066

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6L27_RS10470
• Genome accession: NZ_CP077232
• Coordinates: 2230066..2231184 (+)
• Length: 372 a.a.
• NCBI ID: WP_125739738.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain FDAARGOS 1397
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2225066..2236184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L27_RS10460 (I6L27_10465)	yggS	2228190..2228882 (-)	693	WP_031947571.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6L27_RS10465 (I6L27_10470)	pilT	2229009..2230046 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
I6L27_RS10470 (I6L27_10475)	pilU	2230066..2231184 (+)	1119	WP_125739738.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6L27_RS10475 (I6L27_10480)	fur	2231254..2231691 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6L27_RS10480 (I6L27_10485)	omlA	2231803..2232201 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6L27_RS10485 (I6L27_10490)	-	2232266..2232580 (-)	315	WP_016144536.1	RnfH family protein	-
I6L27_RS10490 (I6L27_10495)	-	2232580..2233647 (-)	1068	WP_216967065.1	hypothetical protein	-
I6L27_RS10495 (I6L27_10500)	-	2233801..2234256 (-)	456	WP_216967068.1	bacteriohemerythrin	-
I6L27_RS10500 (I6L27_10505)	-	2234508..2235356 (-)	849	WP_125698345.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.07 Da
• Isoelectric Point: 6.6401

>NTDB_id=580066 I6L27_RS10470 WP_125739738.1 2230066..2231184(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1397]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=580066 I6L27_RS10470 WP_125739738.1 2230066..2231184(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1397]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
AATGAAGATTAACGGACAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAGCGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGAGATAAATCTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCAGGCATGGTGCTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGTATCATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACCTCATTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATCACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCGCCCGATGTAATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTCGCCGAAACGGGTCACTTAGTATTAGCCACAATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCATTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCCACTCCAGATGGTAATTCGCGCCGCGCCGCAATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCTGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (38-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36