Detailed information NTG0579803

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6L28_RS16855
• Genome accession: NZ_CP077205
• Coordinates: 3588887..3590005 (+)
• Length: 372 a.a.
• NCBI ID: WP_060465360.1
• Uniprot ID: A0A2I6LWN2
• Organism: Acinetobacter pittii strain FDAARGOS 1398
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3583887..3595005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L28_RS16845 (I6L28_16845)	yggS	3587010..3587702 (-)	693	WP_163118392.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6L28_RS16850 (I6L28_16850)	pilT	3587829..3588866 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
I6L28_RS16855 (I6L28_16855)	pilU	3588887..3590005 (+)	1119	WP_060465360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6L28_RS16860 (I6L28_16860)	fur	3590075..3590512 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6L28_RS16865 (I6L28_16865)	omlA	3590624..3591022 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6L28_RS16870 (I6L28_16870)	-	3591087..3591401 (-)	315	WP_017481335.1	RnfH family protein	-
I6L28_RS16875 (I6L28_16875)	-	3591401..3592468 (-)	1068	WP_216946575.1	hypothetical protein	-
I6L28_RS16880 (I6L28_16880)	-	3592622..3593077 (-)	456	WP_002121698.1	bacteriohemerythrin	-
I6L28_RS16885 (I6L28_16885)	-	3593329..3594177 (-)	849	WP_016144533.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=579803 I6L28_RS16855 WP_060465360.1 3588887..3590005(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1398]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=579803 I6L28_RS16855 WP_060465360.1 3588887..3590005(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1398]
ATGGATTTTAACGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTCCGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGTATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACCTCATTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCGCCCGATGTAATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ATGCAATTGGCTTCGCCGAAACGGGTCACTTAGTATTAGCCACAATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCATTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACCTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTTATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGATTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A2I6LWN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363