Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HU754_RS13270 Genome accession   NZ_CP077090
Coordinates   3036666..3037775 (+) Length   369 a.a.
NCBI ID   WP_186621364.1    Uniprot ID   A0A9E6TDH9
Organism   Pseudomonas zeae strain OE 48.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3031666..3042775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS13255 (HU754_013255) - 3032641..3033579 (+) 939 WP_186621362.1 sugar kinase -
  HU754_RS13260 (HU754_013260) - 3033688..3033963 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  HU754_RS13265 (HU754_013265) - 3034015..3036552 (-) 2538 WP_093430942.1 PAS domain S-box protein -
  HU754_RS13270 (HU754_013270) pilU 3036666..3037775 (+) 1110 WP_186621364.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HU754_RS13275 (HU754_013275) - 3037909..3041676 (+) 3768 WP_186621366.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40902.69 Da        Isoelectric Point: 6.4376

>NTDB_id=579049 HU754_RS13270 WP_186621364.1 3036666..3037775(+) (pilU) [Pseudomonas zeae strain OE 48.2]
MEIDALLSILSNKNGSDLFLSTGAPPSARIDGVLTVLSERPFKNGETAAIGASLMDAEQRREFDRDLEMNLAISRAGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPTVLLDTVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAHNANQALDRI
INMFPEDRRPQLLHTLGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTIADLVRRGQFEELKPIMEKSTELGMQTFDAAL
YALVAEGAISAQEALKNADSVNNLKLRMKLSEEADVGNDSTSEEWGLID

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=579049 HU754_RS13270 WP_186621364.1 3036666..3037775(+) (pilU) [Pseudomonas zeae strain OE 48.2]
ATGGAAATCGATGCACTGTTGTCAATTTTGTCCAATAAAAACGGTTCGGACCTGTTCCTCTCCACGGGCGCACCTCCCAG
TGCGCGCATTGACGGCGTGCTCACAGTGTTGAGCGAACGCCCGTTCAAGAACGGTGAAACCGCCGCCATCGGCGCCTCCC
TGATGGACGCCGAGCAACGACGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAGCGGGCATCGGGCGT
TTTCGCGTCAATATCTTCAAGCAGCGCAACGATGTATCGATCGTCATTCGCAATGTCAAACTCGACATCCCGCGTTTTGC
CGATCTGAAATTACCGACAGTACTGCTCGACACCGTCATGCTCAAGCAGGGGTTGATCCTCTTTGTCGGCGCCACCGATT
CGGGCAAGTCGACCTCGCTGGCCGCGCTGATCGACCACCGCAACCGCCATAGCAGTGGGCACATCATCACCATCGAGGAC
CCGATCGAGTACATCCATCGCCACCAGCGCTCGATCATCAATCAACGGGAAGTCGGGGTCGATACACGCAGCTTCCACGC
GGCCCTGAAAAACACCCTGCGCCAGGCACCGGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAAACCATGGAACATG
CCTTGGCGTTTGCCGATACCGGGCATCTGGTGCTCTCGACGTTGCACGCGCACAACGCCAATCAGGCACTGGATCGGATT
ATCAATATGTTCCCGGAAGACCGGCGACCGCAGTTGTTGCATACGCTGGGAAACAACCTGAAAGCATTTATCTCGCAACG
CTTAGTGCGCACCGTCGATGGGCAACGCAGGGCGGCTGTCGAGGTGCTGTTGGGCACGCCAACCATCGCAGATCTGGTTC
GGCGCGGACAGTTCGAGGAGCTGAAACCGATAATGGAAAAATCCACCGAACTGGGTATGCAGACATTTGATGCGGCGCTG
TACGCATTAGTCGCTGAAGGTGCAATCAGTGCGCAGGAAGCTTTAAAGAATGCAGATTCGGTGAATAATTTGAAGTTGCG
GATGAAACTTTCTGAAGAGGCAGATGTTGGTAATGATTCAACGTCCGAAGAATGGGGTTTGATCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.25

99.729

0.561
  pilU Acinetobacter baylyi ADP1

54.062

96.748

0.523
  pilU Vibrio cholerae strain A1552

50.139

97.832

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.244

0.366
  pilT Vibrio cholerae strain A1552

40.541

90.244

0.366