Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSS97_RS03170 Genome accession   NZ_CP077080
Coordinates   715839..717911 (+) Length   690 a.a.
NCBI ID   WP_217861073.1    Uniprot ID   -
Organism   Pseudomonas alvandae strain SWRI17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710839..722911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS97_RS03150 (KSS97_03150) comM 713051..714115 (+) 1065 WP_217861071.1 pilus assembly protein PilM Machinery gene
  KSS97_RS03155 (KSS97_03155) - 714115..714681 (+) 567 WP_217861072.1 PilN domain-containing protein -
  KSS97_RS03160 (KSS97_03160) pilO 714678..715301 (+) 624 WP_030140989.1 type 4a pilus biogenesis protein PilO -
  KSS97_RS03165 (KSS97_03165) - 715298..715825 (+) 528 WP_030140988.1 pilus assembly protein PilP -
  KSS97_RS03170 (KSS97_03170) pilQ 715839..717911 (+) 2073 WP_217861073.1 type IV pilus secretin PilQ Machinery gene
  KSS97_RS03175 (KSS97_03175) aroK 717916..718434 (+) 519 WP_033864550.1 shikimate kinase AroK -
  KSS97_RS03180 (KSS97_03180) aroB 718583..719683 (+) 1101 WP_217861074.1 3-dehydroquinate synthase -
  KSS97_RS03185 (KSS97_03185) - 719694..721271 (+) 1578 WP_217861075.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74574.65 Da        Isoelectric Point: 5.2967

>NTDB_id=578800 KSS97_RS03170 WP_217861073.1 715839..717911(+) (pilQ) [Pseudomonas alvandae strain SWRI17]
MNRIFSALGISLWIALLSPMVQAASLKTLDVAALPGDRVELKLTFDGPPPPPRGYTTEQPARIALDLPGVTNRLASKNHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAARQAAAQAPRPAVASAAPARAAAPAGKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFPRTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPSGTFD
YSTYQTDNKLTVSVRPMTVEDLQKRNSDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNSSGVTTGD
STTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=578800 KSS97_RS03170 WP_217861073.1 715839..717911(+) (pilQ) [Pseudomonas alvandae strain SWRI17]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCAAGCGGCCAGCCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGATCGTGTCGAGTTGAAGCTTACCTTCGACGGGCCGCCGCCGCCGCCCCGGG
GCTATACCACCGAACAGCCGGCCCGTATCGCCTTGGACCTGCCCGGCGTGACCAATCGGTTGGCCAGCAAGAACCATGAT
CTTGGCGGTGGCAATGCACGCAGTGCCACCGTGGTCGAGGCGGGGGACCGGACCCGGCTGATCATCGGCCTTACGCAACT
GGCGCCGTACGACACCCGGATCGAAGGCAACAACCTGTTCGTCGTGGTTGGCAAGGGAGCCGCCCGCCAGGCCGCTGCTC
AGGCTCCGCGTCCTGCGGTTGCCAGCGCCGCGCCTGCCAGGGCCGCTGCGCCGGCCGGCAAGGCGATCCGAGGCGTGGAC
TTTCAGCGCGGAACCCAGGGCGAAGGTAACGTGGTAATCGACTTGTCCGATCCTTCCATTGCCCCGGACATCCAGGAGCG
CGAAGGCAAGATCATCCTCAATTTCCCCAGGACCCAATTGCCCGAACCATTGCGGGTACGCCTGGACGTCAAGGACTTTG
CCACGCCGGTGCAGTTCGTCAATGCCAGCGCCGGTTCCGACCGCGCCACCATCAGCATCGAGCCCAGCGGCACCTTCGAT
TACTCCACCTACCAGACCGACAACAAGCTGACCGTCAGTGTCCGGCCCATGACGGTCGAAGACCTGCAGAAACGCAACAG
CGACCGCGCCGCCTATAGCGGCGAGAAGTTATCGCTGAATTTCCAGGACATCGAAGTGCGCTCGGTGCTGCAACTCATCG
CCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACCGTGCAGGGTGGTATCACCTTGCGGCTGCAGAATGTGCCGTGG
GACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGGCTCGACAAGCGCAAGGTGGGCAATGTGCTGCTGGTGGCCCCGGC
GGATGAAATTGCCGCACGCGAACGCCAGGAGCTGGAATCCCAGAAACAGATCGCCGATCTGGCACCGCTGCGGCGCGAGC
TGTTGCAGGTCAACTATGCCAAGGCGGCCGACATCGCCAAGCTGTTCCAGTCAGTCACCAGTGCCGAGGCGAAGGCAGAT
GAGCGCGGCACGATCACGGTCGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAGTTGCG
CCGGATCGTCGCGCAGCTGGACATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGATTACG
ACAAGAGCCTGGGCGTGCGCTGGGGAGGCTCGGTGCAGAACAAAGGCAACTGGAACTCTTCCGGTGTCACCACGGGCGAT
TCCACCACCATCGGCACGCCGGGCAGTACCAGCACCAACCAGCCGTTCGTCGACCTCGGGGCGTCAGGCAACACCTCCGG
GATCGGCATCGCCTTCATCACCGACAACGTGCTGCTGGACCTGGAACTGACGGCCATGGAAAAAACCGGCAATGGGGAAA
TTGTCTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACGGAGATTCCCTATCAGGAA
GCCAGCTCCAGCGGCGCCACTTCGGTGTCGTTCAAGGAAGCCTCGCTGTCGCTGGAAGTCACGCCGCAGATCACGCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTGCAAGAGGTTCCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGATGGCGAGACCATTGTGATTGGCGGCGTTTTCTCAAATACTCAG
AGCAAAGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.324

100

0.761
  comQ Acinetobacter baylyi ADP1

41.739

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.514

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.514

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.279

97.681

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.279

97.681

0.374