Detailed information NTG0578269

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: KQ255_RS00295
• Genome accession: NZ_CP076821
• Coordinates: 60066..61124 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain UC22850
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 55066..66124

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KQ255_RS00270 (KQ255_00270)	aroK	55341..55910 (-)	570	WP_000643712.1	shikimate kinase AroK	-
KQ255_RS00275 (KQ255_00275)	pilQ	55922..58087 (-)	2166	WP_001017040.1	type IV pilus secretin PilQ family protein	Machinery gene
KQ255_RS00280 (KQ255_00280)	pilP	58150..58677 (-)	528	WP_000695059.1	pilus assembly protein PilP	Machinery gene
KQ255_RS00285 (KQ255_00285)	pilO	58688..59428 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
KQ255_RS00290 (KQ255_00290)	pilN	59425..60066 (-)	642	WP_000201229.1	PilN domain-containing protein	Machinery gene
KQ255_RS00295 (KQ255_00295)	pilM	60066..61124 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
KQ255_RS00300 (KQ255_00300)	ponA	61286..63841 (+)	2556	WP_000736662.1	penicillin-binding protein PBP1a	-
KQ255_RS00305 (KQ255_00305)	-	63859..64683 (-)	825	WP_001990541.1	putative RNA methyltransferase	-
KQ255_RS00310 (KQ255_00310)	-	64686..65018 (-)	333	WP_000169598.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=578269 KQ255_RS00295 WP_002014066.1 60066..61124(-) (pilM) [Acinetobacter baumannii strain UC22850]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=578269 KQ255_RS00295 WP_002014066.1 60066..61124(-) (pilM) [Acinetobacter baumannii strain UC22850]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423