Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KQ249_RS06515 Genome accession   NZ_CP076686
Coordinates   1418960..1421026 (+) Length   688 a.a.
NCBI ID   WP_014576031.1    Uniprot ID   E4PMW4
Organism   Marinobacter adhaerens strain HP15-B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1413960..1426026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ249_RS06495 (KQ249_06495) - 1416109..1417169 (+) 1061 Protein_1287 pilus assembly protein PilM -
  KQ249_RS06500 (KQ249_06500) - 1417169..1417735 (+) 567 WP_014576028.1 PilN domain-containing protein -
  KQ249_RS06505 (KQ249_06505) pilO 1417732..1418343 (+) 612 WP_014576029.1 type 4a pilus biogenesis protein PilO -
  KQ249_RS06510 (KQ249_06510) - 1418346..1418894 (+) 549 WP_041644945.1 pilus assembly protein PilP -
  KQ249_RS06515 (KQ249_06515) pilQ 1418960..1421026 (+) 2067 WP_014576031.1 type IV pilus secretin PilQ Machinery gene
  KQ249_RS06520 (KQ249_06520) aroK 1421163..1421729 (+) 567 WP_008170790.1 shikimate kinase AroK -
  KQ249_RS06525 (KQ249_06525) aroB 1421722..1422816 (+) 1095 WP_014576032.1 3-dehydroquinate synthase -
  KQ249_RS06530 (KQ249_06530) - 1422872..1424359 (+) 1488 WP_014576033.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 74023.95 Da        Isoelectric Point: 4.2604

>NTDB_id=577306 KQ249_RS06515 WP_014576031.1 1418960..1421026(+) (pilQ) [Marinobacter adhaerens strain HP15-B]
MFRKLNVYVSVIAFGLLSGLANAVTLEDVSFSSLPGERLEVTLQFDGAPPEPSGYTIERPARIAVDLRDTTSGLDSRSVP
LGSGNAQSMTVVETKDRTRLIFNLVELVPYDTVRSGNSLIMTIGGQSEGVVASPSTSVSQSSGTTSAAPSDALAGVDFRR
GKDGEGRVLVDLGSSSTPVDLTERAGRIRLTMDGISVPADLRRRLDVTDFATPVTRIDTFVEDGNAVVEISPEGNYDYIA
YQSGSQFTVSVEELSQEEAESRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGSITLRLQNVPWDQA
LDLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETSKQIAELAPVRLDIVQVNYAKAADIVALIKEDEELISDRGFVS
SDVRTNTISVRETAEKLEEIRRLVSTWDVPVRQVSIEARIVRAQTNVAENLGVRWGGAAYDVSGDNVFTVGGSQGSLQEA
RDAAAGNSNTISFPGALAVDLGVSGEGTSSFAIGWGSDNFLVDLELSALESDGQAEVVSQPRVVTADRQTASIKSGEEIP
YQEATSSGATNIEFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNSVTTQVLVGNGETVVLGGIFQSE
VATQTTKTPFLGDIPYLGRIFKRTEHLDERSELLIFITPKIIKNDLLR

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=577306 KQ249_RS06515 WP_014576031.1 1418960..1421026(+) (pilQ) [Marinobacter adhaerens strain HP15-B]
ATGTTCAGAAAACTCAATGTATACGTCAGCGTGATTGCTTTTGGGTTGTTATCCGGCCTGGCCAACGCGGTCACGCTGGA
AGACGTGTCGTTTTCGTCGCTACCAGGCGAGCGGCTTGAGGTGACACTGCAATTTGATGGGGCGCCACCGGAACCCTCTG
GTTATACCATTGAGCGCCCCGCTCGAATCGCGGTGGATCTTCGGGATACGACCAGTGGTCTTGATAGCCGAAGCGTGCCT
TTGGGGTCGGGTAATGCCCAGAGCATGACCGTGGTCGAGACCAAGGATCGAACGCGGTTGATTTTCAACCTTGTCGAGCT
GGTTCCCTATGACACAGTTCGTTCTGGTAACTCCCTGATCATGACGATTGGTGGGCAGTCTGAGGGTGTTGTTGCGAGCC
CCTCAACCTCCGTTTCGCAGAGCAGTGGAACAACCAGCGCGGCGCCTTCTGATGCTCTTGCCGGGGTCGATTTCCGTCGC
GGCAAAGACGGCGAAGGCCGAGTGCTGGTGGATCTTGGCAGCTCCAGCACGCCGGTCGACCTGACAGAACGTGCCGGCAG
AATTCGTCTTACGATGGATGGTATTTCTGTGCCTGCCGATCTGCGTCGCCGGCTCGACGTGACGGATTTCGCCACGCCGG
TTACTCGCATTGACACCTTTGTGGAAGATGGCAATGCCGTTGTCGAGATCAGCCCTGAGGGTAACTACGACTACATTGCC
TACCAGTCTGGCAGCCAGTTTACCGTCAGCGTCGAGGAGCTAAGCCAGGAAGAGGCCGAATCCCGCCGGGAAGAGAAGTT
CCCGTATACCGGCGACAAGCTCTCCCTGAACTTCCAGGACATCGAAGTTCGCTCAGTGCTTCAGCTTATCGCCGACTTCA
CCGGATTGAACCTCGTGGCCAGTGACACTGTCGGCGGCAGCATCACTCTGCGCCTTCAGAATGTGCCCTGGGACCAGGCA
CTTGATCTGATTCTGAAGACAAAAGGTCTTGATAAGCGTCAGATCGGTAACGTTTTGCTCGTCGCGCCTGCAGATGAGAT
CGCAGCTAGGGAGAAGCTTGAGCTTGAAACTAGCAAGCAGATTGCTGAGTTGGCTCCTGTGCGCCTCGATATCGTTCAGG
TCAACTATGCCAAGGCGGCTGATATTGTTGCTTTGATTAAGGAAGATGAGGAACTGATTTCTGATCGGGGTTTTGTGTCC
TCTGATGTTAGAACGAACACGATCAGTGTCAGGGAGACCGCGGAAAAGTTGGAGGAAATTCGCAGGCTGGTTTCAACCTG
GGATGTTCCTGTGCGTCAAGTCTCTATCGAGGCTCGTATAGTGCGGGCTCAAACGAATGTTGCCGAGAACCTGGGTGTTC
GATGGGGTGGTGCTGCCTACGATGTGAGTGGCGATAACGTGTTTACAGTCGGTGGTTCCCAGGGATCGCTGCAGGAGGCT
CGTGACGCAGCTGCCGGGAATAGCAACACGATTTCTTTCCCGGGAGCTCTTGCGGTTGATCTTGGTGTTAGTGGTGAGGG
TACCTCCTCCTTTGCCATTGGATGGGGTAGTGACAACTTCCTCGTTGATCTGGAGTTATCCGCTTTGGAAAGCGATGGCC
AGGCCGAGGTTGTTTCTCAGCCTAGGGTCGTTACTGCTGATCGCCAAACAGCATCTATCAAATCAGGTGAGGAAATCCCT
TACCAAGAGGCAACTTCCAGTGGCGCGACCAATATAGAATTTAAAGAAGCGGTTCTTTCACTGGAAGTCACACCTCAAAT
CACGCCTGACGACAAAATCATTATGGACTTGGTAGTAAACCAGGATTCCAGAGGTGAAGTTACGGCTGGCATTCCGTCTA
TCAATACGAACTCTGTTACTACACAGGTTCTGGTTGGTAACGGCGAGACAGTAGTTCTGGGTGGTATTTTCCAGTCTGAG
GTTGCGACTCAAACGACCAAGACGCCGTTCCTTGGAGATATCCCTTACCTGGGCCGTATCTTCAAGCGCACTGAGCACCT
TGACGAGCGTAGCGAACTGTTGATCTTCATTACGCCGAAGATCATCAAGAACGATCTGCTTCGTTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(297-343)

Bacterial type II and III secretion system protein (Secretin)

(528-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-433)

AMIN domain (AMIN)

(38-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4PMW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.801

100

0.503
  pilQ Acinetobacter baumannii strain A118

39.398

100

0.419
  comQ Acinetobacter baylyi ADP1

40.453

100

0.416
  pilQ Acinetobacter baumannii D1279779

39.178

100

0.416
  pilQ Legionella pneumophila strain Lp02

41.037

98.11

0.403
  pilQ Legionella pneumophila strain ERS1305867

41.037

98.11

0.403