Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KNV97_RS21160 Genome accession   NZ_CP076643
Coordinates   3248693..3250501 (-) Length   602 a.a.
NCBI ID   WP_136486093.1    Uniprot ID   -
Organism   Vibrio ostreae strain OG9-811     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243693..3255501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNV97_RS21135 (KNV97_21135) yacG 3244928..3245125 (-) 198 WP_136486081.1 DNA gyrase inhibitor YacG -
  KNV97_RS21140 (KNV97_21140) zapD 3245162..3245902 (-) 741 WP_136486084.1 cell division protein ZapD -
  KNV97_RS21145 (KNV97_21145) coaE 3245934..3246542 (-) 609 WP_218562704.1 dephospho-CoA kinase -
  KNV97_RS21150 (KNV97_21150) pilD 3246545..3247414 (-) 870 WP_218562705.1 A24 family peptidase Machinery gene
  KNV97_RS21155 (KNV97_21155) pilC 3247465..3248682 (-) 1218 WP_218562706.1 type II secretion system F family protein Machinery gene
  KNV97_RS21160 (KNV97_21160) pilB 3248693..3250501 (-) 1809 WP_136486093.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  KNV97_RS21165 (KNV97_21165) - 3250501..3250911 (-) 411 WP_218562707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KNV97_RS21170 (KNV97_21170) nadC 3251085..3251972 (-) 888 WP_218562708.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KNV97_RS21175 (KNV97_21175) ampD 3252115..3252663 (+) 549 WP_218562709.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KNV97_RS21180 (KNV97_21180) fldB 3252693..3253214 (-) 522 WP_218562710.1 flavodoxin FldB -
  KNV97_RS21185 (KNV97_21185) xerD 3253352..3254260 (+) 909 WP_136486103.1 site-specific tyrosine recombinase XerD -
  KNV97_RS21190 (KNV97_21190) dsbC 3254276..3255037 (+) 762 WP_136486105.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 66997.61 Da        Isoelectric Point: 6.2780

>NTDB_id=576840 KNV97_RS21160 WP_136486093.1 3248693..3250501(-) (pilB) [Vibrio ostreae strain OG9-811]
MSTQLLRQLRQAGVLDGEQETALQRQTLSRHISAPQAILEQGVLTPAELSQQLSRLFSLPLIELDDFDYPALCQQLGLSE
LIIRYQALPLQRDSRELTLAVSDPGWPQAEEEFCFATGLMVKPVLTDQPSLQAAIRRLYGETLPSYHAERREEISQEQLA
DAVQVAEDEANYQLDVSQDEAPVSRFIHQVLLDAVRKRASDIHFEPYESSYRIRIRCDGILIQTQQPAPHLSRRLAARLK
ILARLDIAQRRLPQDGRIKLRLNNDSAIDLRVSTLPTLWGEKIVLRLLDNQALPLELDQLGYSKQQKQCYLQALHKPQGM
ILITGPTGSGKTISLYCGLQQLNREHLNIATAEDPVEINLNGINQVQVQPAIGFGFAQALRTFLRQDPDVLMVGEIRDRE
TADVALKAAQTGHLVLSTLHTNSAAETLIRLLNMGIEPFNLASSLNLIVAQRLVRRLCPQCKIATQLTRAQADLLHIESL
HIESRPLTHSPAASAPAHSAQEQQQQTTEQRKSVTDTVIFAANPDGCPHCNRGYAGRTGIYEMLNVEPVIADALLQRAPV
KQLESLACQQGMQTLQQAGCEKLLAGQTSLAELQRVLGLATW

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=576840 KNV97_RS21160 WP_136486093.1 3248693..3250501(-) (pilB) [Vibrio ostreae strain OG9-811]
ATGAGCACCCAATTACTCCGCCAGTTGCGTCAGGCCGGCGTCCTTGATGGCGAGCAGGAGACAGCGCTGCAGCGCCAGAC
GCTGAGCCGCCATATCAGTGCGCCACAAGCGATCCTTGAACAAGGCGTGCTGACCCCGGCCGAGCTCAGCCAGCAACTGA
GTCGTCTGTTCAGCCTGCCGCTGATTGAACTCGACGACTTTGACTACCCGGCTTTGTGTCAGCAACTGGGCCTGAGCGAG
CTGATCATTCGTTATCAGGCGCTGCCCCTGCAGCGTGACAGTCGGGAGTTAACCCTGGCCGTATCGGATCCCGGCTGGCC
CCAAGCGGAAGAAGAGTTCTGTTTTGCCACCGGTCTGATGGTTAAACCGGTGCTGACTGATCAGCCCAGCCTGCAAGCAG
CGATACGACGTCTGTATGGCGAGACACTCCCCAGCTATCATGCCGAGCGCCGCGAAGAGATCAGCCAGGAACAGCTCGCC
GATGCGGTACAGGTTGCCGAAGATGAAGCCAATTATCAGTTGGATGTCAGCCAGGACGAAGCACCGGTCAGCCGATTCAT
TCATCAGGTGTTACTCGATGCGGTACGCAAACGGGCCTCGGATATCCATTTTGAACCTTACGAGTCGAGTTATCGCATCC
GCATTCGCTGTGACGGCATCTTGATTCAGACCCAGCAACCGGCTCCGCACCTCAGTCGCCGCCTGGCAGCGCGTCTGAAA
ATTCTTGCCCGGCTGGATATCGCCCAGCGCCGTCTGCCGCAAGATGGTCGGATCAAACTGCGCCTCAATAATGACAGCGC
CATTGACCTGCGTGTATCCACTCTGCCGACCTTATGGGGCGAAAAGATCGTCCTGCGCCTGCTCGATAATCAGGCTCTGC
CGCTTGAACTGGACCAGCTTGGTTACAGCAAGCAGCAAAAACAGTGCTACCTGCAGGCTCTACACAAACCACAGGGCATG
ATCCTGATCACCGGACCAACCGGCAGCGGTAAGACCATTTCGTTATACTGCGGGCTGCAGCAGCTCAATCGTGAACACCT
CAATATTGCTACCGCAGAAGATCCGGTCGAGATCAACCTCAACGGGATTAACCAGGTCCAAGTCCAGCCGGCGATCGGAT
TTGGCTTTGCCCAGGCCCTGCGTACATTTCTGCGTCAGGATCCGGATGTACTCATGGTGGGTGAGATCCGCGATCGCGAG
ACCGCAGACGTTGCACTCAAAGCCGCTCAGACCGGGCATCTGGTCCTATCCACTCTGCATACCAATTCAGCCGCAGAGAC
TTTGATTCGTTTACTGAATATGGGCATTGAACCTTTTAATCTGGCATCGTCGCTGAATCTTATCGTGGCTCAGCGTCTGG
TACGCCGCTTATGCCCGCAGTGTAAAATCGCCACCCAACTGACCCGGGCGCAGGCCGACTTACTTCATATAGAAAGCCTT
CATATAGAAAGCCGTCCTCTAACCCACTCTCCTGCCGCAAGCGCGCCGGCTCATTCAGCACAAGAGCAGCAGCAACAGAC
AACTGAGCAGCGCAAGTCGGTAACAGACACGGTGATCTTTGCCGCCAACCCGGACGGCTGTCCCCACTGTAATCGTGGCT
ACGCCGGACGCACCGGTATCTATGAAATGCTGAATGTTGAACCTGTCATTGCCGATGCACTGCTACAGCGCGCACCTGTG
AAGCAGCTGGAGTCGCTGGCTTGTCAGCAGGGCATGCAGACTTTGCAGCAAGCCGGCTGTGAAAAACTGCTCGCTGGGCA
GACCAGCCTGGCGGAGCTGCAGCGGGTACTGGGTCTGGCGACCTGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

60.469

99.169

0.6
  pilB Vibrio parahaemolyticus RIMD 2210633

59.296

99.169

0.588
  pilB Vibrio campbellii strain DS40M4

57.86

99.336

0.575
  pilB Acinetobacter baylyi ADP1

43.121

99.003

0.427
  pilB Acinetobacter baumannii D1279779

44.902

92.857

0.417
  pilF Neisseria gonorrhoeae MS11

40.568

99.502

0.404
  pilB Legionella pneumophila strain ERS1305867

39.832

98.837

0.394