Detailed information NTG0575069

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JNUCC22_RS12115
• Genome accession: NZ_CP076408
• Coordinates: 2521931..2522545 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus sp. JNUCC-22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2516931..2527545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JNUCC22_RS12085 (JNUCC22_12085)	rpsT	2517234..2517500 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JNUCC22_RS12090 (JNUCC22_12090)	holA	2517517..2518558 (-)	1042	Protein_2354	DNA polymerase III subunit delta	-
JNUCC22_RS12095 (JNUCC22_12095)	-	2518598..2518749 (-)	152	Protein_2355	hypothetical protein	-
JNUCC22_RS12100 (JNUCC22_12100)	-	2518790..2518924 (+)	135	WP_003152870.1	YqzM family protein	-
JNUCC22_RS12105 (JNUCC22_12105)	comEC	2518943..2521294 (-)	2352	WP_216061754.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JNUCC22_RS12110 (JNUCC22_12110)	-	2521295..2521864 (-)	570	WP_003152868.1	ComE operon protein 2	-
JNUCC22_RS12115 (JNUCC22_12115)	comEA	2521931..2522545 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
JNUCC22_RS12120 (JNUCC22_12120)	comER	2522604..2523425 (+)	822	WP_053103971.1	late competence protein ComER	-
JNUCC22_RS12125 (JNUCC22_12125)	-	2523494..2524231 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
JNUCC22_RS12130 (JNUCC22_12130)	rsfS	2524228..2524584 (-)	357	WP_007408260.1	ribosome silencing factor	-
JNUCC22_RS12135 (JNUCC22_12135)	yqeK	2524601..2525161 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JNUCC22_RS12140 (JNUCC22_12140)	-	2525151..2525720 (-)	570	WP_042635364.1	nicotinate-nucleotide adenylyltransferase	-
JNUCC22_RS12145 (JNUCC22_12145)	yhbY	2525731..2526021 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JNUCC22_RS12150 (JNUCC22_12150)	aroE	2526015..2526857 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=575069 JNUCC22_RS12115 WP_032866693.1 2521931..2522545(-) (comEA) [Bacillus sp. JNUCC-22]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=575069 JNUCC22_RS12115 WP_032866693.1 2521931..2522545(-) (comEA) [Bacillus sp. JNUCC-22]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363