Detailed information NTG0574553

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KM843_RS12110
• Genome accession: NZ_CP076291
• Coordinates: 2506684..2507298 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain US1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501684..2512298

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KM843_RS12080 (KM843_12080)	rpsT	2501987..2502253 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KM843_RS12085 (KM843_12085)	holA	2502270..2503311 (-)	1042	Protein_2350	DNA polymerase III subunit delta	-
KM843_RS12090 (KM843_12090)	-	2503351..2503502 (-)	152	Protein_2351	hypothetical protein	-
KM843_RS12095 (KM843_12095)	-	2503543..2503677 (+)	135	WP_003152870.1	YqzM family protein	-
KM843_RS12100 (KM843_12100)	comEC	2503696..2506047 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KM843_RS12105 (KM843_12105)	-	2506048..2506617 (-)	570	WP_003152868.1	ComE operon protein 2	-
KM843_RS12110 (KM843_12110)	comEA	2506684..2507298 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
KM843_RS12115 (KM843_12115)	comER	2507357..2508178 (+)	822	WP_012118027.1	late competence protein ComER	-
KM843_RS12120 (KM843_12120)	-	2508247..2508984 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
KM843_RS12125 (KM843_12125)	rsfS	2508981..2509337 (-)	357	WP_007408260.1	ribosome silencing factor	-
KM843_RS12130 (KM843_12130)	yqeK	2509354..2509914 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KM843_RS12135 (KM843_12135)	-	2509904..2510473 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
KM843_RS12140 (KM843_12140)	yhbY	2510484..2510774 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
KM843_RS12145 (KM843_12145)	aroE	2510768..2511610 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=574553 KM843_RS12110 WP_012118026.1 2506684..2507298(-) (comEA) [Bacillus velezensis strain US1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=574553 KM843_RS12110 WP_012118026.1 2506684..2507298(-) (comEA) [Bacillus velezensis strain US1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363