Detailed information NTG0574066

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: KM563_RS06165
• Genome accession: NZ_CP076253
• Coordinates: 1376439..1378175 (+)
• Length: 578 a.a.
• NCBI ID: WP_009580420.1
• Uniprot ID: A0A1M4J5J2
• Organism: Xanthomonas translucens pv. graminis strain ART-Xtg2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1375274..1389011	1376439..1378175	within	0

Gene organization within MGE regions

Location: 1375274..1389011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KM563_RS06150	-	1375274..1375525 (-)	252	WP_009580414.1	transposase	-
KM563_RS06155 (KM563_06115)	-	1375625..1375945 (-)	321	WP_009580416.1	DUF86 domain-containing protein	-
KM563_RS06160 (KM563_06120)	-	1375942..1376232 (-)	291	WP_009580418.1	nucleotidyltransferase family protein	-
KM563_RS06165 (KM563_06125)	pilB	1376439..1378175 (+)	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KM563_RS06170 (KM563_06130)	-	1378203..1378496 (-)	294	WP_009580422.1	addiction module antidote protein	-
KM563_RS06175 (KM563_06135)	-	1378499..1378795 (-)	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KM563_RS06180 (KM563_06140)	pilR	1378960..1380348 (-)	1389	WP_009580426.1	sigma-54-dependent transcriptional regulator	Regulator
KM563_RS06185 (KM563_06145)	-	1380423..1382036 (-)	1614	WP_009580428.1	sensor histidine kinase	-
KM563_RS06190 (KM563_06150)	sucC	1382335..1383498 (+)	1164	WP_009580518.1	ADP-forming succinate--CoA ligase subunit beta	-
KM563_RS06195 (KM563_06155)	sucD	1383522..1384394 (+)	873	WP_009580520.1	succinate--CoA ligase subunit alpha	-
KM563_RS06200 (KM563_06160)	-	1384794..1386887 (+)	2094	WP_009580522.1	pyrroloquinoline quinone-dependent dehydrogenase	-
KM563_RS06205 (KM563_06165)	-	1387128..1389011 (-)	1884	WP_009580524.1	hypothetical protein	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62918.20 Da
• Isoelectric Point: 6.1466

>NTDB_id=574066 KM563_RS06165 WP_009580420.1 1376439..1378175(+) (pilB) [Xanthomonas translucens pv. graminis strain ART-Xtg2]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKYQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=574066 KM563_RS06165 WP_009580420.1 1376439..1378175(+) (pilB) [Xanthomonas translucens pv. graminis strain ART-Xtg2]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGTGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCCGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGTGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACACTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCTGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATTGCGGCGATCGTAT
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (51-152)

3D structure

• AlphaFold DB: A0A1M4J5J2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.93	98.27	0.54
pilB	Acinetobacter baumannii D1279779	53.927	99.135	0.535
pilB	Legionella pneumophila strain ERS1305867	52.373	98.443	0.516
pilB	Vibrio cholerae strain A1552	49.047	99.827	0.49
pilF	Neisseria gonorrhoeae MS11	48.592	98.27	0.478
pilB	Vibrio parahaemolyticus RIMD 2210633	46.82	97.924	0.458
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.421	90.311	0.365
pilF	Thermus thermophilus HB27	40.385	89.965	0.363