Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KM547_RS14560 Genome accession   NZ_CP076251
Coordinates   3337758..3339494 (-) Length   578 a.a.
NCBI ID   WP_053838004.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. phlei strain LMG 730     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3332758..3344494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM547_RS14540 (KM547_14480) - 3333897..3335510 (+) 1614 WP_053838003.1 sensor histidine kinase -
  KM547_RS14545 (KM547_14485) pilR 3335585..3336973 (+) 1389 WP_003477091.1 sigma-54-dependent transcriptional regulator Regulator
  KM547_RS14550 (KM547_14490) - 3337138..3337434 (+) 297 WP_053833736.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM547_RS14555 (KM547_14495) - 3337437..3337730 (+) 294 WP_009580422.1 addiction module antidote protein -
  KM547_RS14560 (KM547_14500) pilB 3337758..3339494 (-) 1737 WP_053838004.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM547_RS14565 (KM547_14505) - 3339701..3339991 (+) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KM547_RS14570 (KM547_14510) - 3339988..3340359 (+) 372 WP_053838005.1 DUF86 domain-containing protein -
  KM547_RS14575 (KM547_14515) - 3340495..3341052 (+) 558 WP_053838006.1 transposase -
  KM547_RS14580 (KM547_14520) - 3341286..3341735 (-) 450 WP_053838007.1 pilin -
  KM547_RS14585 (KM547_14525) pilC 3342121..3343380 (+) 1260 WP_053838008.1 type II secretion system F family protein Machinery gene
  KM547_RS14590 (KM547_14530) - 3343387..3344250 (+) 864 WP_053833732.1 prepilin peptidase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62980.27 Da        Isoelectric Point: 6.0951

>NTDB_id=574045 KM547_RS14560 WP_053838004.1 3337758..3339494(-) (pilB) [Xanthomonas translucens pv. phlei strain LMG 730]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKHQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGVDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEEEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNEEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=574045 KM547_RS14560 WP_053838004.1 3337758..3339494(-) (pilB) [Xanthomonas translucens pv. phlei strain LMG 730]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAATTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGTCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGTTATGAGGAAGAACAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGTCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGAAGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.577

98.27

0.536
  pilB Acinetobacter baumannii D1279779

54.225

98.27

0.533
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

48.7

99.827

0.486
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.996

97.924

0.46
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.23

90.311

0.363