Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCNPT3_RS11600 Genome accession   NC_020802
Coordinates   2631712..2633403 (-) Length   563 a.a.
NCBI ID   WP_015466049.1    Uniprot ID   M4UNT2
Organism   Psychromonas sp. CNPT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2626712..2638403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCNPT3_RS11585 (PCNPT3_11155) nagA 2627600..2628733 (+) 1134 WP_015466046.1 N-acetylglucosamine-6-phosphate deacetylase -
  PCNPT3_RS11590 (PCNPT3_11160) - 2629050..2630273 (+) 1224 WP_015466047.1 ROK family protein -
  PCNPT3_RS11595 (PCNPT3_11165) pilC 2630458..2631690 (-) 1233 WP_015466048.1 type II secretion system F family protein Machinery gene
  PCNPT3_RS11600 (PCNPT3_11170) pilB 2631712..2633403 (-) 1692 WP_015466049.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCNPT3_RS14355 (PCNPT3_11175) - 2633415..2633819 (-) 405 WP_015466050.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCNPT3_RS11610 (PCNPT3_11180) - 2634330..2634764 (+) 435 WP_015466051.1 transcriptional repressor -
  PCNPT3_RS11615 (PCNPT3_11185) - 2634824..2635288 (+) 465 WP_015466052.1 chemotaxis protein CheX -
  PCNPT3_RS11620 (PCNPT3_11190) - 2635446..2636120 (-) 675 WP_015466053.1 hypothetical protein -
  PCNPT3_RS11625 (PCNPT3_11195) - 2636232..2636675 (-) 444 WP_015466054.1 TerB family tellurite resistance protein -
  PCNPT3_RS11630 (PCNPT3_11200) - 2637082..2637699 (+) 618 WP_015466055.1 thiol:disulfide interchange protein DsbA/DsbL -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62129.30 Da        Isoelectric Point: 5.1458

>NTDB_id=57374 PCNPT3_RS11600 WP_015466049.1 2631712..2633403(-) (pilB) [Psychromonas sp. CNPT3]
MSIHKISGLALCLATHGLLATDKLQQITDSAASEQKSLVSYLVENKLIDSLALAQFCEMEYGIPLLSSSSLELDNIPEKF
LNEKLIEKHHALPIYVQQNTLYIAISDPTNLDALEDFGFSFSMHTEALLVEEEFLKKAIEAKIDSGDLVFDDSAIGDINE
VEVDDKDSRLDENQNGDDDAPIVKYINSILLDAVRKGASDLHFEPYEKKYRIRFRIDGILTEIATPPVNLANRFSARLKV
MSKLDIAERRLPQDGRIKLRISKKRSVDLRVSTLPTIWGEKVVMRILDSSASSLDINILGYSDAQKDKYIKALAKPQGMI
LITGPTGSGKTVSLYTGLSILNTVERNISTAEDPVEINLTGINQVQINNKAGLTFASALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSASETLTRLLNMGVEGFNIASSVALIIAQRLARRLCPECKVEDKYSDAQLQKFGFTAQQ
IEEGGMIYKPVGCSKCNEGYKGRVGIYEVMPFSENIGRIILSNGNALQIEKAARDEGMYTLRESALQKAMLGLIGLKEVA
RIT

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=57374 PCNPT3_RS11600 WP_015466049.1 2631712..2633403(-) (pilB) [Psychromonas sp. CNPT3]
ATGTCTATACATAAAATAAGTGGCCTTGCCTTGTGCTTGGCCACACATGGTTTATTAGCGACCGATAAACTGCAACAAAT
CACCGACAGCGCTGCCAGTGAGCAAAAGTCGCTGGTCTCTTATTTGGTTGAGAATAAATTAATTGACAGCCTCGCGCTGG
CACAATTTTGTGAAATGGAATATGGCATTCCCTTATTATCCTCATCGAGTTTAGAGCTCGATAATATTCCTGAAAAGTTT
TTAAATGAAAAGTTAATTGAAAAACACCACGCATTGCCTATTTACGTGCAACAAAACACCTTATATATCGCTATATCTGA
TCCCACCAACTTAGACGCATTAGAAGACTTTGGGTTTAGCTTCTCGATGCACACGGAAGCGTTGCTTGTTGAAGAAGAAT
TTCTAAAAAAAGCCATTGAAGCCAAGATTGACAGTGGTGACCTTGTTTTTGATGATTCGGCTATTGGCGACATCAATGAA
GTCGAAGTGGATGACAAAGATTCTCGCTTAGATGAAAATCAAAATGGTGATGATGATGCGCCCATTGTAAAATACATTAA
TAGCATTCTACTTGACGCCGTGCGCAAAGGCGCCTCAGATTTGCATTTTGAACCTTATGAAAAAAAATACCGTATCCGTT
TTCGTATCGATGGCATCTTAACGGAGATTGCCACCCCCCCTGTTAATTTAGCTAACCGTTTTTCAGCGCGTCTTAAAGTC
ATGTCAAAACTTGATATCGCTGAGCGTAGGCTTCCTCAAGATGGGCGTATTAAACTGCGTATCTCTAAAAAGCGCAGTGT
GGATCTGCGGGTCAGTACCTTACCGACAATATGGGGCGAAAAAGTGGTGATGCGTATTCTCGACTCGTCTGCGAGCAGTT
TAGATATTAATATATTGGGTTACAGTGACGCGCAAAAAGATAAATATATTAAGGCCTTAGCCAAACCTCAAGGCATGATT
TTAATCACAGGGCCAACAGGTAGTGGTAAAACCGTCTCCTTGTATACGGGGCTGAGCATTTTAAATACCGTTGAGCGCAA
TATATCAACGGCGGAAGATCCCGTTGAAATAAATTTAACCGGTATCAATCAAGTACAAATAAATAACAAAGCGGGCTTAA
CGTTTGCCTCTGCCTTACGCTCCTTCTTACGTCAAGATCCCGATGTGGTGATGGTCGGTGAAATACGTGATTTAGAAACA
GCTGAAATTGCTATAAAAGCGGCCCAAACAGGGCATTTAGTCTTATCGACATTGCATACTAACTCGGCATCTGAAACACT
AACGCGTTTATTAAATATGGGCGTTGAAGGCTTTAATATTGCCTCTTCTGTCGCACTTATCATTGCGCAGCGTTTAGCGC
GCAGGCTTTGCCCTGAGTGTAAAGTTGAAGATAAATATAGCGATGCACAATTACAAAAGTTTGGTTTTACTGCGCAGCAA
ATAGAGGAAGGGGGCATGATTTACAAGCCCGTAGGTTGCTCTAAATGTAATGAGGGTTATAAAGGGCGTGTAGGTATATA
TGAAGTAATGCCATTTTCGGAAAACATTGGGCGCATTATTTTAAGTAACGGCAATGCACTGCAGATTGAAAAAGCTGCCA
GAGACGAAGGCATGTATACACTGCGAGAATCTGCATTACAAAAAGCGATGTTAGGCTTGATCGGCTTAAAAGAAGTGGCG
CGTATTACTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-139)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4UNT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.641

100

0.531
  pilB Acinetobacter baylyi ADP1

52.827

100

0.531
  pilB Acinetobacter baumannii D1279779

52.205

100

0.526
  pilB Vibrio cholerae strain A1552

50.97

100

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.337

99.645

0.512
  pilB Vibrio campbellii strain DS40M4

50.977

100

0.51
  pilF Neisseria gonorrhoeae MS11

48.126

90.053

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.128

100

0.377
  pilF Thermus thermophilus HB27

39.464

92.718

0.366

Multiple sequence alignment