Detailed information NTG0571621

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KH238_RS10640
• Genome accession: NZ_CP076045
• Coordinates: 2233619..2234233 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain VCN56
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2228619..2239233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KH238_RS10610 (KH238_10610)	rpsT	2228922..2229188 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KH238_RS10615 (KH238_10615)	holA	2229205..2230246 (-)	1042	Protein_2103	DNA polymerase III subunit delta	-
KH238_RS10620 (KH238_10620)	-	2230304..2230437 (-)	134	Protein_2104	hypothetical protein	-
KH238_RS10625 (KH238_10625)	-	2230478..2230612 (+)	135	WP_003152870.1	YqzM family protein	-
KH238_RS10630 (KH238_10630)	comEC	2230631..2232982 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KH238_RS10635 (KH238_10635)	-	2232983..2233552 (-)	570	WP_003152868.1	ComE operon protein 2	-
KH238_RS10640 (KH238_10640)	comEA	2233619..2234233 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
KH238_RS10645 (KH238_10645)	comER	2234292..2235113 (+)	822	WP_032866690.1	late competence protein ComER	-
KH238_RS10650 (KH238_10650)	-	2235182..2235919 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
KH238_RS10655 (KH238_10655)	rsfS	2235916..2236272 (-)	357	WP_007408260.1	ribosome silencing factor	-
KH238_RS10660 (KH238_10660)	yqeK	2236290..2236850 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KH238_RS10665 (KH238_10665)	-	2236840..2237409 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
KH238_RS10670 (KH238_10670)	yhbY	2237420..2237710 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KH238_RS10675 (KH238_10675)	aroE	2237704..2238546 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=571621 KH238_RS10640 WP_032866693.1 2233619..2234233(-) (comEA) [Bacillus velezensis strain VCN56]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=571621 KH238_RS10640 WP_032866693.1 2233619..2234233(-) (comEA) [Bacillus velezensis strain VCN56]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363