Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KJF94_RS28580 Genome accession   NZ_CP075566
Coordinates   6142074..6144161 (+) Length   695 a.a.
NCBI ID   WP_214380305.1    Uniprot ID   -
Organism   Pseudomonas hormoni strain G20-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6137074..6149161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJF94_RS28560 (KJF94_28560) comM 6139289..6140353 (+) 1065 WP_214380301.1 pilus assembly protein PilM Machinery gene
  KJF94_RS28565 (KJF94_28565) - 6140353..6140916 (+) 564 WP_214380302.1 PilN domain-containing protein -
  KJF94_RS28570 (KJF94_28570) pilO 6140913..6141536 (+) 624 WP_214380303.1 type 4a pilus biogenesis protein PilO -
  KJF94_RS28575 (KJF94_28575) - 6141533..6142060 (+) 528 WP_214380304.1 pilus assembly protein PilP -
  KJF94_RS28580 (KJF94_28580) pilQ 6142074..6144161 (+) 2088 WP_214380305.1 type IV pilus secretin PilQ Machinery gene
  KJF94_RS28585 (KJF94_28585) aroK 6144166..6144684 (+) 519 WP_017336125.1 shikimate kinase AroK -
  KJF94_RS28590 (KJF94_28590) aroB 6144836..6145936 (+) 1101 WP_214380306.1 3-dehydroquinate synthase -
  KJF94_RS28595 (KJF94_28595) - 6145947..6147545 (+) 1599 WP_214380307.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75280.61 Da        Isoelectric Point: 5.7805

>NTDB_id=569725 KJF94_RS28580 WP_214380305.1 6142074..6144161(+) (pilQ) [Pseudomonas hormoni strain G20-18]
MNRIFATFGLSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGAPPTPHGYTTEQPARIALDLPGVVSKLANKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYTARVEGNNLFVVVGRGTSNAASTPAAKPPRVATATPAPVKAYTPVSKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIVVGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRAIISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVS
NGANASSTIGTPGSTSTNSPFVDMGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=569725 KJF94_RS28580 WP_214380305.1 6142074..6144161(+) (pilQ) [Pseudomonas hormoni strain G20-18]
ATGAACAGGATTTTCGCAACCTTCGGACTATCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTCGCTGCGCTGCCAGGGGACCGCGTCGAGTTGAAGTTGTCGTTCGATGGAGCGCCCCCGACACCCCACG
GTTACACGACGGAGCAGCCTGCGCGGATTGCCCTGGATCTGCCCGGCGTTGTCAGCAAGCTGGCGAACAAGACCCGTGAT
CTTGGCGGTGGCAACGCCCGCAGCGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCAACCTGACTCAATT
GACCCCGTACACCGCTCGCGTCGAAGGCAATAATCTGTTTGTGGTGGTCGGGCGCGGAACCAGTAACGCAGCCTCCACAC
CCGCAGCCAAACCGCCACGTGTCGCCACGGCAACTCCGGCGCCGGTCAAGGCCTATACGCCAGTGAGCAAGGCGATCCGC
GGCGTGGATTTCCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTCATCGATCTGTCGGATCCGTCCATTGCCCCGGACAT
TCAGGAGCGCGACGGCAAGATTGTCGTGGGTTTCGCCAGGACCCAATTGCCGGAACCCTTGCGGGTCCGTCTGGACGTCA
AGGATTTCGCCACGCCCGTGCAATTCGTCAACGCCAGCGCCACGGGAGACCGTGCGATCATCAGCATTGAGCCCAGCGGC
GCGTTCGATTACTCGACCTATCAGACCGACAACAAGCTGACCGTCAGCATCCGCCCGATGACCGTAGACGACTTGCAGAA
GCGCAACGCGGAGCGTTTTGCCTACACCGGGGAAAAGCTCTCGCTGAACTTCCAGGACATTGACGTGCGCTCGGTGCTGC
AACTGATTGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACCTTGCGCTTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGGCTGGACAAACGCAAGATCGGCAACGTGCTGCTGGT
GGCACCGGCCGATGAGATTGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCACTGC
GACGCGAGCTGCTGCAAGTCAACTACGCCAAGGCGGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCG
AAAGTCGACGAACGGGGTTCGATTACGGTCGATGAACGAACCAATAACATCATTGCCTATCAGACCCAGGACCGCCTTGA
CGAGCTGCGCCGTATCGTCGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCACGGATTGTCGAAGCGAACG
TCGACTACGACAAAAGCCTTGGTGTGCGATGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACACGTCCGGGGTGAGC
AACGGCGCCAATGCGTCGTCGACCATCGGTACGCCCGGCAGCACCAGCACCAACTCACCGTTCGTCGACATGGGCACCAC
CGCCAACACCTCGGGGCTCGGCATCGCTTTCATCACCGATAATGTGCTGCTGGATCTTGAGCTGACCGCGATGGAAAAAA
CCGGTAACGGTGAAATCGTCTCGCAACCCAAGGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACGGAG
ATTCCGTATCAGGAAGCCAGCTCCAGTGGTGCTACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTAACGCC
GCAGATCACGCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGGTGCAGG
ATGTACCGCCAATCAAGAAAAACGAGGTCAACGCCAAGGTCCTCGTCAATGACGGCGAGACCATCGTGATCGGTGGTGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGA
GTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.753
  comQ Acinetobacter baylyi ADP1

42.489

100

0.427
  pilQ Acinetobacter baumannii D1279779

38.462

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.462

100

0.417
  pilQ Legionella pneumophila strain ERS1305867

38.201

97.554

0.373
  pilQ Legionella pneumophila strain Lp02

38.201

97.554

0.373