Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MMZ72_RS09380 Genome accession   NZ_CP093021
Coordinates   2015518..2017026 (+) Length   502 a.a.
NCBI ID   WP_024915005.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2010518..2022026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS09360 (MMZ72_09360) - 2010549..2011760 (+) 1212 WP_024915003.1 O-succinylhomoserine sulfhydrylase -
  MMZ72_RS09365 (MMZ72_09365) - 2011757..2012524 (+) 768 WP_003091381.1 SDR family oxidoreductase -
  MMZ72_RS09370 (MMZ72_09370) xcpQ 2012610..2014586 (-) 1977 WP_121305552.1 GspD family T2SS secretin variant XcpQ -
  MMZ72_RS09375 (MMZ72_09375) xcpP 2014591..2015298 (-) 708 WP_003119750.1 type II secretion system protein N -
  MMZ72_RS09380 (MMZ72_09380) pilB 2015518..2017026 (+) 1509 WP_024915005.1 GspE family T2SS ATPase variant XcpR Machinery gene
  MMZ72_RS09385 (MMZ72_09385) xcpS 2017026..2018243 (+) 1218 WP_024915006.1 GspF family T2SS innner membrane protein variant XcpS -
  MMZ72_RS09390 (MMZ72_09390) xcpT 2018248..2018694 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  MMZ72_RS09395 (MMZ72_09395) xcpU 2018701..2019219 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  MMZ72_RS09400 (MMZ72_09400) xcpV 2019216..2019605 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  MMZ72_RS09405 (MMZ72_09405) xcpW 2019602..2020315 (+) 714 WP_058139327.1 GspJ family T2SS minor pseudopilin variant XcpW -
  MMZ72_RS09410 (MMZ72_09410) xcpX 2020312..2021313 (+) 1002 WP_024915009.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55514.69 Da        Isoelectric Point: 6.2074

>NTDB_id=569659 MMZ72_RS09380 WP_024915005.1 2015518..2017026(+) (pilB) [Pseudomonas aeruginosa strain H09]
MMTAPLPDIAAPAASPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=569659 MMZ72_RS09380 WP_024915005.1 2015518..2017026(+) (pilB) [Pseudomonas aeruginosa strain H09]
ATGATGACAGCCCCGTTACCCGACATCGCCGCGCCGGCCGCATCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTCGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAAGTCATGGCGCGGCTGGATATCGC
CGAAAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGGGTGGTGCTGCGTCTGCTCGACAAACAGGCCGGGCGCCTCAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACCGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACGCTTTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAATATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAAGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCCTC
GCTGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCATCGGTGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCATCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGCCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367