Detailed information NTG0569469

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KHS93_RS12300
• Genome accession: NZ_CP075547
• Coordinates: 2550802..2551416 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain SN16-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545802..2556416

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHS93_RS12270 (KHS93_12270)	rpsT	2546102..2546368 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KHS93_RS12275 (KHS93_12275)	holA	2546385..2547426 (-)	1042	Protein_2389	DNA polymerase III subunit delta	-
KHS93_RS12280 (KHS93_12280)	-	2547466..2547620 (-)	155	Protein_2390	hypothetical protein	-
KHS93_RS12285 (KHS93_12285)	-	2547661..2547795 (+)	135	WP_003152870.1	YqzM family protein	-
KHS93_RS12290 (KHS93_12290)	comEC	2547814..2550165 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KHS93_RS12295 (KHS93_12295)	-	2550166..2550735 (-)	570	WP_003152868.1	ComE operon protein 2	-
KHS93_RS12300 (KHS93_12300)	comEA	2550802..2551416 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
KHS93_RS12305 (KHS93_12305)	comER	2551475..2552296 (+)	822	WP_014305449.1	late competence protein ComER	-
KHS93_RS12310 (KHS93_12310)	-	2552365..2553102 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
KHS93_RS12315 (KHS93_12315)	rsfS	2553099..2553455 (-)	357	WP_003152864.1	ribosome silencing factor	-
KHS93_RS12320 (KHS93_12320)	yqeK	2553452..2554033 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KHS93_RS12325 (KHS93_12325)	-	2554023..2554592 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
KHS93_RS12330 (KHS93_12330)	yhbY	2554603..2554893 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KHS93_RS12335 (KHS93_12335)	aroE	2554887..2555729 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=569469 KHS93_RS12300 WP_003152867.1 2550802..2551416(-) (comEA) [Bacillus amyloliquefaciens strain SN16-1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=569469 KHS93_RS12300 WP_003152867.1 2550802..2551416(-) (comEA) [Bacillus amyloliquefaciens strain SN16-1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363