Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MMZ77_RS22285 Genome accession   NZ_CP093014
Coordinates   4841370..4842779 (-) Length   469 a.a.
NCBI ID   WP_029610467.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4836370..4847779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ77_RS22270 (MMZ77_22275) - 4837338..4838450 (-) 1113 WP_003112720.1 substrate-binding domain-containing protein -
  MMZ77_RS22275 (MMZ77_22280) lapA 4838536..4839642 (-) 1107 WP_003112123.1 alkaline phosphatase LapA -
  MMZ77_RS22280 (MMZ77_22285) pilC 4840156..4841370 (-) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  MMZ77_RS22285 (MMZ77_22290) pilB 4841370..4842779 (-) 1410 WP_029610467.1 type II secretion system ATPase GspE Machinery gene
  MMZ77_RS22290 (MMZ77_22295) gspD 4842776..4845187 (-) 2412 WP_024947465.1 type II secretion system secretin GspD -
  MMZ77_RS22295 (MMZ77_22300) gspM 4845184..4845777 (-) 594 WP_009315211.1 type II secretion system protein M -
  MMZ77_RS22300 (MMZ77_22305) gspL 4845764..4846975 (-) 1212 WP_023119786.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 51028.42 Da        Isoelectric Point: 5.7943

>NTDB_id=569284 MMZ77_RS22285 WP_029610467.1 4841370..4842779(-) (pilB) [Pseudomonas aeruginosa strain H17]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDFREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=569284 MMZ77_RS22285 WP_029610467.1 4841370..4842779(-) (pilB) [Pseudomonas aeruginosa strain H17]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGGACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACTGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCGGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTTGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGATGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTCGACAAGCAGGCCG
GGCGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGATCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGACACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACCCGGCTGGTGGACATGGGCGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCCTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATTTCCGCGAAGCCGCCCGCGCGGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.076

84.222

0.388
  pilB Vibrio parahaemolyticus RIMD 2210633

45.823

84.222

0.386
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373