Detailed information NTG0568532

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: KI610_RS16635
• Genome accession: NZ_CP075189
• Coordinates: 3474829..3476544 (+)
• Length: 571 a.a.
• NCBI ID: WP_226496063.1
• Uniprot ID: -
• Organism: Ferribacterium limneticum strain 77
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3465110..3477786	3474829..3476544	within	0

Gene organization within MGE regions

Location: 3465110..3477786

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KI610_RS16575 (KI610_16490)	-	3465110..3465511 (-)	402	WP_226496053.1	polymer-forming cytoskeletal protein	-
KI610_RS16580 (KI610_16495)	-	3465523..3466101 (-)	579	WP_226496054.1	DUF6776 family protein	-
KI610_RS16585 (KI610_16500)	rpsI	3466435..3466827 (-)	393	WP_226403796.1	30S ribosomal protein S9	-
KI610_RS16590 (KI610_16505)	rplM	3466837..3467265 (-)	429	WP_117607868.1	50S ribosomal protein L13	-
KI610_RS16595 (KI610_16510)	-	3467769..3468197 (+)	429	WP_226496055.1	OsmC family protein	-
KI610_RS16600 (KI610_16515)	coq7	3468214..3468837 (-)	624	WP_226496056.1	2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase	-
KI610_RS16605 (KI610_16520)	-	3468842..3469357 (-)	516	WP_226496057.1	CNP1-like family protein	-
KI610_RS16610 (KI610_16525)	-	3469360..3469908 (-)	549	WP_226496058.1	RNA pyrophosphohydrolase	-
KI610_RS16615 (KI610_16530)	-	3470019..3471749 (+)	1731	WP_226496059.1	proline--tRNA ligase	-
KI610_RS16620 (KI610_16535)	-	3471737..3472348 (+)	612	WP_226496060.1	lytic transglycosylase domain-containing protein	-
KI610_RS16625 (KI610_16540)	ffh	3472418..3473773 (-)	1356	WP_226496061.1	signal recognition particle protein	-
KI610_RS16630 (KI610_16545)	-	3473803..3474642 (+)	840	WP_226496062.1	inner membrane protein YpjD	-
KI610_RS16635 (KI610_16550)	pilB	3474829..3476544 (+)	1716	WP_226496063.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KI610_RS16640 (KI610_16555)	pilC	3476560..3477786 (+)	1227	WP_226496064.1	type II secretion system F family protein	Machinery gene

Sequence

Protein

• Length: 571 a.a.
• Molecular weight: 61759.21 Da
• Isoelectric Point: 5.4465

>NTDB_id=568532 KI610_RS16635 WP_226496063.1 3474829..3476544(+) (pilB) [Ferribacterium limneticum strain 77]
MAATPQNPPLGGLARALVQAGQLKEAEAEQLLAQAHSSKTSLIEQIIISQKAGALDIARFVADTFGYPLLDLAAFDEAHI
PSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEQSKLAPLVAKYAESAADALKNFTSE
DINLDFLDEEAAGKADEVAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMRLVLSKNRAIDFRVSTLPTLQGEKIVMRILDPTSATLGIEALGYEPEQKATLLEAIS
RPYGMILVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPITVPEQALLDAG
YAEADLDGSWTLFGPSGCDRCKGTGYKGRVGIYQVMPISEAMQRLILSGATALDLGAQAKAEGVKNLRESGLLKVKQGMT
SLDEVLSTTNA

Nucleotide

• Length: 1716 bp

>NTDB_id=568532 KI610_RS16635 WP_226496063.1 3474829..3476544(+) (pilB) [Ferribacterium limneticum strain 77]
ATGGCAGCAACACCTCAAAATCCTCCACTCGGCGGCCTGGCACGAGCCCTTGTACAGGCCGGGCAACTCAAGGAGGCCGA
GGCAGAACAATTGCTGGCACAAGCTCACAGCAGCAAGACATCGCTGATCGAGCAGATCATAATCAGCCAAAAAGCCGGCG
CGCTCGATATCGCGCGTTTCGTTGCCGACACCTTTGGCTATCCACTACTCGATCTCGCCGCATTCGATGAGGCGCACATC
CCGTCAGACGCGATCGACAGAAAGCTGATCGCCACCCACAAAGTCATCCCGCTCAACAAGCGTGGCAACCGTCTTTCGGT
CGCCATCGCCGACCCGACCAATCTGCGCGCCCTCGACGAAATACGCTTTCAGACCGGACTCGCTGTCGACCCCATCGTCG
TCGAGCAATCGAAACTCGCCCCTCTGGTTGCCAAGTACGCCGAATCCGCTGCGGATGCACTCAAGAATTTCACCAGCGAA
GACATCAACCTCGACTTTCTCGACGAAGAAGCGGCAGGCAAAGCTGACGAAGTGGCCGGCCAGGAGATTGATGATGCTCC
GGTTGTCAAATTCATTCAGAAAATGTTGCTGGATGCCATCAACGACGGCGCTTCCGACATCCACTTCGAACCCTACGAAA
AGTTCTATCGCATCCGCTTTCGGGTCGACGGTATCTTGCGTGAAGTCGCCACGCCCCCCTTGGCGATCAAGGAAAAAATC
GCCTCGCGAATCAAGGTCATTTCCCGACTGAATATTGCCGAAAAGCGTGTCCCCCAAGATGGGCGGATGCGACTCGTATT
ATCGAAGAATCGGGCCATCGATTTCCGGGTCAGTACCCTGCCGACATTGCAGGGCGAAAAGATCGTTATGCGGATTCTCG
ACCCTACTTCCGCCACATTGGGCATTGAGGCACTCGGCTATGAACCGGAACAGAAAGCCACTCTGCTGGAAGCCATCAGC
CGGCCTTACGGAATGATTCTGGTGACCGGGCCAACCGGCTCCGGCAAAACGGTATCGCTCTACACCTGCCTGAACATCCT
GAACAAGGATGGCATCAATATTTCGACGGCCGAAGATCCTGCTGAAATAAACCTCCCCGGCGTCAATCAGGTTAACGTCG
ACGACCGCGCGGGACTAACTTTCCCCGTCGCGCTCAAAGCCTTTCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAA
ATCCGCGACCTGGAAACCGCAGAGATATCGATCAAGGCTGCCCAGACAGGCCACCTTGTACTTTCGACACTGCATACAAA
TGACGCACCGCAAACCTTGACCCGCCTGATGAACATGGGAGTCCCCATGTTCAACATTGCCTCCAGCGTTCTCCTGATCA
CCGCTCAACGTTTGGCCCGTCGACTCTGCAATTGCAAAAAACCCATCACAGTTCCAGAACAGGCACTTCTTGATGCGGGG
TATGCGGAGGCAGACCTCGATGGATCCTGGACACTGTTCGGCCCGAGCGGTTGCGATCGCTGCAAAGGTACTGGCTACAA
AGGGCGAGTTGGCATCTATCAGGTCATGCCAATATCCGAGGCCATGCAGCGCCTGATCCTGAGCGGAGCCACTGCGCTTG
ATCTGGGCGCCCAAGCCAAGGCTGAAGGGGTGAAAAACCTGCGCGAATCCGGTTTGTTGAAGGTAAAACAAGGCATGACG
TCACTTGACGAAGTCTTAAGCACTACCAACGCCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-150)

• Type II/IV secretion system protein (T2SSE) (194-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.895	100	0.55
pilB	Acinetobacter baumannii D1279779	53.147	100	0.532
pilB	Legionella pneumophila strain ERS1305867	51.93	99.825	0.518
pilF	Neisseria gonorrhoeae MS11	52.212	98.949	0.517
pilB	Vibrio parahaemolyticus RIMD 2210633	48.399	98.424	0.476
pilB	Vibrio cholerae strain A1552	48.479	97.898	0.475
pilB	Vibrio campbellii strain DS40M4	47.957	98.599	0.473
pilF	Thermus thermophilus HB27	39.619	100	0.401
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.869	99.124	0.385