Detailed information NTG0567561

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KIV13_RS12345
• Genome accession: NZ_CP075055
• Coordinates: 2550241..2550855 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain YYC
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545241..2555855

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KIV13_RS12315 (KIV13_12315)	rpsT	2545544..2545810 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KIV13_RS12320 (KIV13_12320)	holA	2545827..2546868 (-)	1042	Protein_2397	DNA polymerase III subunit delta	-
KIV13_RS12325 (KIV13_12325)	-	2546903..2547059 (-)	157	Protein_2398	hypothetical protein	-
KIV13_RS12330 (KIV13_12330)	-	2547100..2547234 (+)	135	WP_003152870.1	YqzM family protein	-
KIV13_RS12335 (KIV13_12335)	comEC	2547253..2549604 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KIV13_RS12340 (KIV13_12340)	-	2549605..2550174 (-)	570	WP_003152868.1	ComE operon protein 2	-
KIV13_RS12345 (KIV13_12345)	comEA	2550241..2550855 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
KIV13_RS12350 (KIV13_12350)	comER	2550914..2551735 (+)	822	WP_032866690.1	late competence protein ComER	-
KIV13_RS12355 (KIV13_12355)	-	2551804..2552541 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
KIV13_RS12360 (KIV13_12360)	rsfS	2552538..2552894 (-)	357	WP_007408260.1	ribosome silencing factor	-
KIV13_RS12365 (KIV13_12365)	yqeK	2552912..2553472 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KIV13_RS12370 (KIV13_12370)	-	2553462..2554031 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
KIV13_RS12375 (KIV13_12375)	yhbY	2554042..2554332 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KIV13_RS12380 (KIV13_12380)	aroE	2554326..2555168 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=567561 KIV13_RS12345 WP_032866693.1 2550241..2550855(-) (comEA) [Bacillus velezensis strain YYC]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=567561 KIV13_RS12345 WP_032866693.1 2550241..2550855(-) (comEA) [Bacillus velezensis strain YYC]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363