Detailed information NTG0056490

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LILO_RS10885
• Genome accession: NC_020450
• Coordinates: 2251699..2252145 (-)
• Length: 148 a.a.
• NCBI ID: WP_042230641.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis IO-1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2246699..2257145

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LILO_RS10850 (lilo_2093)	-	2246816..2247193 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LILO_RS10855 (lilo_2094)	-	2247398..2248264 (+)	867	WP_042230639.1	RluA family pseudouridine synthase	-
LILO_RS10860 (lilo_2095)	-	2248302..2249111 (-)	810	WP_015427158.1	metal ABC transporter permease	-
LILO_RS10865 (lilo_2096)	-	2249104..2249841 (-)	738	WP_015427159.1	metal ABC transporter ATP-binding protein	-
LILO_RS10870 (lilo_2097)	-	2250018..2250860 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LILO_RS10875 (lilo_2098)	-	2250857..2251294 (-)	438	WP_015427161.1	zinc-dependent MarR family transcriptional regulator	-
LILO_RS10880 (lilo_2099)	comGG	2251376..2251660 (-)	285	WP_015427162.1	competence type IV pilus minor pilin ComGG	Machinery gene
LILO_RS10885 (lilo_2100)	comGF	2251699..2252145 (-)	447	WP_042230641.1	competence type IV pilus minor pilin ComGF	Machinery gene
LILO_RS10890 (lilo_2101)	comGE	2252108..2252404 (-)	297	WP_015427164.1	competence type IV pilus minor pilin ComGE	Machinery gene
LILO_RS10895 (lilo_2102)	comGD	2252376..2252807 (-)	432	WP_015427165.1	competence type IV pilus minor pilin ComGD	Machinery gene
LILO_RS10900 (lilo_2103)	comGC	2252767..2253150 (-)	384	WP_015427166.1	competence type IV pilus major pilin ComGC	Machinery gene
LILO_RS10905 (lilo_2104)	comGB	2253164..2254237 (-)	1074	WP_080619357.1	competence type IV pilus assembly protein ComGB	Machinery gene
LILO_RS10910 (lilo_2105)	comGA	2254131..2255069 (-)	939	WP_015427168.1	competence type IV pilus ATPase ComGA	Machinery gene
LILO_RS11780	-	2255339..2256295 (-)	957	WP_052312061.1	hypothetical protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17169.94 Da
• Isoelectric Point: 8.2376

>NTDB_id=56490 LILO_RS10885 WP_042230641.1 2251699..2252145(-) (comGF) [Lactococcus lactis subsp. lactis IO-1]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVEISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=56490 LILO_RS10885 WP_042230641.1 2251699..2252145(-) (comGF) [Lactococcus lactis subsp. lactis IO-1]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTTATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGAGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (46-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385