Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHW15_RS01800 Genome accession   NZ_CP074410
Coordinates   387677..389782 (+) Length   701 a.a.
NCBI ID   WP_024649426.1    Uniprot ID   -
Organism   Pseudomonas syringae strain U643     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 382677..394782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW15_RS01780 (KHW15_01780) pilM 384840..385904 (+) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  KHW15_RS01785 (KHW15_01785) - 385904..386485 (+) 582 WP_003316651.1 PilN domain-containing protein -
  KHW15_RS01790 (KHW15_01790) pilO 386482..387105 (+) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  KHW15_RS01795 (KHW15_01795) pilP 387102..387629 (+) 528 WP_213569141.1 pilus assembly protein PilP -
  KHW15_RS01800 (KHW15_01800) pilQ 387677..389782 (+) 2106 WP_024649426.1 type IV pilus secretin PilQ Machinery gene
  KHW15_RS01805 (KHW15_01805) aroK 389787..390305 (+) 519 WP_003341387.1 shikimate kinase AroK -
  KHW15_RS01810 (KHW15_01810) aroB 390421..391524 (+) 1104 WP_003422530.1 3-dehydroquinate synthase -
  KHW15_RS01815 (KHW15_01815) - 391535..393151 (+) 1617 WP_057407102.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75424.82 Da        Isoelectric Point: 5.1826

>NTDB_id=564675 KHW15_RS01800 WP_024649426.1 387677..389782(+) (pilQ) [Pseudomonas syringae strain U643]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGSGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=564675 KHW15_RS01800 WP_024649426.1 387677..389782(+) (pilQ) [Pseudomonas syringae strain U643]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGCGATCGCATCGAGCTGAAGCTGGCATTCGATGCCCCGGTGCCTGCACCGCGCG
GTTACACCACGTCGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAT
CTGGGCAGCGGCAATGCGCGCAGTGTGGTTGTGGTTCAGGCTCAGGACCGCACTCGGGTGATCATCAACCTGACCACGCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTCGCGGTGCCGCCGCCCACGCCGGCATCCGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACGATCAGCGTGCGTCCATTGAC
CAACGAGGACGTCGACCGCCGCGCCGCTGACAAACCGGCGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGTGGTATC
ACCCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGCTCGATCACGGTGGACGACCGAACCAACAACATCATTGCCTACCA
GACCCAGGAACGGCTCGACGAGCTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTTCGCTGGGGCGGCTCTACGAACACCAGCGGTAACAGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTT
CGTGGACCTTGGCGCAGCCGGCGCCACCTCGGGTATCGGCCTTGGTTTCGTGACCAACAACACGTTGCTGGACCTTGAAC
TGAGTGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGTGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTACTGATTTCCGACGGCGAGACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCTGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.056

100

0.75
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.205

98.573

0.377
  pilQ Legionella pneumophila strain Lp02

38.171

98.288

0.375