Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PA257_RS34665 Genome accession   NZ_AP014651
Coordinates   5681845..5682384 (-) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NCGM257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5676845..5687384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA257_RS26810 (PA257_5595) - 5677530..5679785 (-) 2256 WP_033990664.1 DUF1631 domain-containing protein -
  PA257_RS26815 (PA257_5596) nadC 5680071..5680919 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PA257_RS26825 - 5681083..5681781 (-) 699 WP_223677410.1 hypothetical protein -
  PA257_RS34665 pilE 5681845..5682384 (-) 540 WP_033938465.1 pilin Machinery gene
  PA257_RS26830 (PA257_5600) pilB 5682613..5684316 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA257_RS26835 (PA257_5601) pilC 5684320..5685537 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PA257_RS26840 (PA257_5602) pilD 5685540..5686412 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA257_RS26845 (PA257_5603) coaE 5686409..5687020 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PA257_RS26850 (PA257_5604) yacG 5687017..5687217 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=56196 PA257_RS34665 WP_033938465.1 5681845..5682384(-) (pilE) [Pseudomonas aeruginosa strain NCGM257]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=56196 PA257_RS34665 WP_033938465.1 5681845..5682384(-) (pilE) [Pseudomonas aeruginosa strain NCGM257]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363

Multiple sequence alignment