Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFF03_RS14485 Genome accession   NZ_CP073745
Coordinates   3188857..3190554 (+) Length   565 a.a.
NCBI ID   WP_255857628.1    Uniprot ID   A0A9J7ACR0
Organism   Bacterioplanoides sp. SCSIO 12839     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3183857..3195554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFF03_RS14455 (KFF03_14400) ampD 3184283..3184822 (-) 540 WP_255857624.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFF03_RS14460 (KFF03_14405) - 3185027..3186394 (+) 1368 WP_255857625.1 sodium:alanine symporter family protein -
  KFF03_RS14465 (KFF03_14410) nadC 3186531..3187379 (+) 849 WP_255857626.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KFF03_RS14470 (KFF03_14415) - 3187397..3188062 (+) 666 WP_255857627.1 hypothetical protein -
  KFF03_RS14480 - 3188289..3188747 (+) 459 WP_304941505.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KFF03_RS14485 (KFF03_14425) pilB 3188857..3190554 (+) 1698 WP_255857628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFF03_RS14490 (KFF03_14430) pilC 3190595..3191821 (+) 1227 WP_255857629.1 type II secretion system F family protein Machinery gene
  KFF03_RS14495 (KFF03_14435) pilD 3191822..3192718 (+) 897 WP_255857630.1 A24 family peptidase Machinery gene
  KFF03_RS14500 (KFF03_14440) coaE 3192734..3193339 (+) 606 WP_255857631.1 dephospho-CoA kinase -
  KFF03_RS14505 (KFF03_14445) yacG 3193365..3193553 (+) 189 WP_255857632.1 DNA gyrase inhibitor YacG -
  KFF03_RS14510 (KFF03_14450) - 3193646..3195292 (+) 1647 WP_255857633.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61659.57 Da        Isoelectric Point: 4.8181

>NTDB_id=560384 KFF03_RS14485 WP_255857628.1 3188857..3190554(+) (pilB) [Bacterioplanoides sp. SCSIO 12839]
MSGISGLPKRLIEQGYINQDQALQIIESTKKDKISFVTAAVKSGQIKSHAIASLASEEFGLPLLDLAAFDFQSAPQDIVS
HNLIEKHRVLPLFSRGTRLFIAQSDPSAIIAVDEIKFNTGMNIEPVLVEEDKLSIAVDKFLSANDNDFGDLEDELGDVDL
EDPEAEAKRREAEQDSAGDDAPIVKFVNKMLLDAIKMGSSDLHFEPYEKRYRVRYRTDGVLHEVASPPVNLAGKIAARLK
VMSKMDISERRIPQDGRIKMKISKNKAIDFRVNTLPTLFGEKIVLRILDPSSAQLGIDMLGYEPEQKALYMEALGKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFASALRSFLRQDPDVVMVGEIRDLE
TASIAIKAAQTGHMVMSTLHTNSAPETLTRLANMGVPNFSIATSVSLIIAQRLARRLCSSCKLPADLPKEVLQEAGFTDE
LLAEAEIYKPVGCDHCKEGFKGRVGIYEVVKVTDPISRIIMEGGNAIEIADAAQKEGFNDLRTSALIKVAQGITSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=560384 KFF03_RS14485 WP_255857628.1 3188857..3190554(+) (pilB) [Bacterioplanoides sp. SCSIO 12839]
ATGAGTGGCATATCAGGTTTACCGAAGCGTTTGATTGAACAGGGTTATATTAACCAAGATCAAGCACTTCAAATCATAGA
AAGCACCAAGAAAGATAAAATCAGCTTTGTTACTGCAGCAGTCAAATCTGGACAAATTAAGTCACATGCAATTGCTTCTC
TTGCATCAGAGGAGTTCGGGCTTCCACTCCTCGACTTAGCAGCTTTCGATTTTCAGTCTGCACCTCAAGATATCGTAAGC
CACAACCTTATTGAAAAGCATCGTGTCTTACCACTATTTAGCCGTGGCACCCGTTTATTTATTGCTCAAAGTGATCCTTC
GGCCATCATCGCTGTTGATGAAATCAAATTTAATACTGGAATGAATATTGAGCCGGTACTGGTTGAAGAGGACAAACTAT
CCATTGCTGTGGATAAGTTTTTGAGTGCCAATGATAATGACTTTGGTGACCTAGAAGATGAATTAGGAGATGTCGACCTC
GAAGACCCCGAGGCAGAAGCAAAAAGGCGCGAGGCAGAGCAAGACTCTGCAGGTGACGATGCACCTATTGTAAAATTCGT
GAATAAAATGCTATTAGATGCTATCAAGATGGGGTCTTCCGACTTACATTTTGAGCCCTATGAGAAACGCTATCGAGTGC
GATATCGTACAGATGGCGTATTGCATGAGGTTGCCAGCCCTCCGGTGAATCTGGCAGGTAAAATTGCCGCCCGACTCAAA
GTCATGTCGAAGATGGATATTTCCGAACGCCGTATCCCGCAGGACGGCCGGATTAAGATGAAAATATCCAAAAATAAGGC
CATCGACTTCCGGGTGAATACGCTACCAACTCTGTTTGGTGAGAAAATTGTTCTTCGGATTTTGGATCCGTCCTCCGCTC
AGTTAGGTATTGATATGCTGGGTTATGAGCCTGAACAAAAAGCGCTCTATATGGAGGCGTTGGGTAAGCCTCAGGGTATG
ATTCTGGTTACAGGGCCAACAGGTTCGGGCAAAACTGTTTCGTTATATACCGGCCTGAATATTCTGAATGTGGAAGGCAC
CAATATTTCCACTGCCGAAGACCCGGTAGAAATCAACCTGGAAGGTATTAACCAGGTGAATGTGAATGCCAGGGTAGGTC
TAGACTTCGCTTCGGCTTTACGCTCATTCTTACGCCAAGACCCTGACGTGGTCATGGTTGGAGAGATCAGGGATCTGGAA
ACCGCTTCAATCGCCATTAAAGCAGCGCAAACTGGCCATATGGTGATGTCAACTCTACATACCAACAGCGCCCCAGAAAC
CTTGACGCGTTTAGCCAATATGGGCGTGCCGAACTTCAGTATTGCCACCTCTGTTAGCCTGATTATTGCCCAGCGCCTGG
CACGACGGCTATGCTCAAGTTGTAAATTGCCTGCCGACCTGCCTAAAGAGGTACTACAAGAAGCTGGTTTTACTGACGAA
CTTCTGGCAGAAGCAGAAATCTATAAACCAGTAGGCTGCGATCATTGCAAAGAAGGTTTTAAAGGCCGTGTGGGAATATA
TGAAGTAGTTAAAGTTACTGACCCCATATCTCGTATTATTATGGAAGGAGGCAATGCCATTGAAATTGCCGATGCAGCCC
AAAAAGAAGGGTTTAATGACTTGCGTACTTCAGCATTGATAAAAGTTGCACAAGGCATAACCAGCTTGGAAGAAGCAAAC
CGAGTGACCACCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

63.186

100

0.632
  pilB Acinetobacter baylyi ADP1

62.191

100

0.623
  pilB Legionella pneumophila strain ERS1305867

53.24

100

0.538
  pilB Vibrio parahaemolyticus RIMD 2210633

49.823

100

0.499
  pilB Vibrio campbellii strain DS40M4

48.94

100

0.49
  pilF Neisseria gonorrhoeae MS11

48.33

100

0.487
  pilB Vibrio cholerae strain A1552

52.427

91.15

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.286

94.159

0.37