Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFF03_RS14495 Genome accession   NZ_CP073745
Coordinates   3191822..3192718 (+) Length   298 a.a.
NCBI ID   WP_255857630.1    Uniprot ID   A0A9J7AJZ6
Organism   Bacterioplanoides sp. SCSIO 12839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3186822..3197718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFF03_RS14470 (KFF03_14415) - 3187397..3188062 (+) 666 WP_255857627.1 hypothetical protein -
  KFF03_RS14480 - 3188289..3188747 (+) 459 WP_304941505.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KFF03_RS14485 (KFF03_14425) pilB 3188857..3190554 (+) 1698 WP_255857628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFF03_RS14490 (KFF03_14430) pilC 3190595..3191821 (+) 1227 WP_255857629.1 type II secretion system F family protein Machinery gene
  KFF03_RS14495 (KFF03_14435) pilD 3191822..3192718 (+) 897 WP_255857630.1 A24 family peptidase Machinery gene
  KFF03_RS14500 (KFF03_14440) coaE 3192734..3193339 (+) 606 WP_255857631.1 dephospho-CoA kinase -
  KFF03_RS14505 (KFF03_14445) yacG 3193365..3193553 (+) 189 WP_255857632.1 DNA gyrase inhibitor YacG -
  KFF03_RS14510 (KFF03_14450) - 3193646..3195292 (+) 1647 WP_255857633.1 NAD(P)/FAD-dependent oxidoreductase -
  KFF03_RS14515 (KFF03_14455) - 3195369..3195791 (-) 423 WP_255857634.1 copper chaperone PCu(A)C -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 32739.22 Da        Isoelectric Point: 6.0816

>NTDB_id=560386 KFF03_RS14495 WP_255857630.1 3191822..3192718(+) (pilD) [Bacterioplanoides sp. SCSIO 12839]
MSLFEVLAAEPIALFIAVIVLSLLVGSFLNVVILRFPVMMFRSWKQECQDFNENMPTHPALEDLSKPYNLIKPDSHCPKC
QAPVRAWQNIPIISYLLLKGQCAGCGTRIGVRYPAVELVTALMSAMLLLVYPWGWPLAAMLIFTWLLISMSVIDIDHQIL
PDSMTLGLMWIGLILNSSLLTPEGLFTDLESAVLGAVLGYGSLWSVFWIFKLVTGKDGMGFGDFKLLAALGAWLGYTALP
LIILLSSVVGAVVGIAGIMILGRDKNLPIPFGPYLAMAGWIAALWGEKITHWYLNSVL

Nucleotide


Download         Length: 897 bp        

>NTDB_id=560386 KFF03_RS14495 WP_255857630.1 3191822..3192718(+) (pilD) [Bacterioplanoides sp. SCSIO 12839]
ATGAGTTTGTTTGAGGTACTAGCAGCAGAACCTATCGCGCTTTTTATTGCCGTAATTGTTCTTTCTCTGCTGGTTGGCAG
CTTCCTGAATGTAGTGATTCTGCGCTTTCCGGTGATGATGTTTCGCAGTTGGAAGCAAGAGTGTCAGGACTTTAATGAGA
ATATGCCGACTCATCCGGCGCTGGAAGACTTATCCAAACCTTACAACCTGATTAAACCCGATTCTCATTGCCCCAAATGC
CAGGCTCCAGTGCGTGCTTGGCAGAATATTCCTATCATCAGTTATCTGTTGTTAAAAGGCCAATGTGCGGGGTGTGGTAC
TCGTATTGGTGTTCGCTACCCGGCTGTTGAGCTGGTGACAGCCCTGATGAGTGCTATGCTGTTGCTAGTTTATCCCTGGG
GGTGGCCGCTGGCAGCCATGCTGATCTTTACCTGGCTTCTGATCAGCATGTCGGTGATCGATATTGATCACCAAATCCTG
CCTGATTCGATGACGCTTGGCTTAATGTGGATAGGACTTATCCTGAATTCCTCATTATTAACCCCAGAAGGATTATTCAC
TGATCTGGAGAGTGCCGTGCTGGGAGCCGTATTAGGCTATGGTTCTTTGTGGTCGGTTTTCTGGATATTTAAACTGGTTA
CCGGTAAAGATGGTATGGGGTTTGGTGACTTCAAATTATTGGCCGCACTCGGAGCCTGGTTGGGGTATACCGCCCTGCCG
TTAATTATTCTGCTGTCATCCGTCGTCGGTGCTGTTGTTGGTATCGCCGGAATCATGATTCTGGGCCGTGATAAAAACCT
GCCGATCCCTTTTGGCCCTTACCTGGCAATGGCAGGCTGGATCGCGGCGCTATGGGGTGAGAAGATCACTCATTGGTACC
TCAATTCCGTTTTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(142-255)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

52.313

94.295

0.493
  pilD Acinetobacter baumannii D1279779

48.227

94.631

0.456
  pilD Acinetobacter nosocomialis M2

47.872

94.631

0.453
  pilD Vibrio cholerae strain A1552

48.201

93.289

0.45
  pilD Vibrio campbellii strain DS40M4

43.878

98.658

0.433