Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KB879_RS28585 Genome accession   NZ_CP073677
Coordinates   6139619..6141349 (+) Length   576 a.a.
NCBI ID   WP_211980320.1    Uniprot ID   -
Organism   Cupriavidus sp. KK10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6134619..6146349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KB879_RS28555 (KB879_28555) obgE 6135477..6136574 (-) 1098 WP_211980319.1 GTPase ObgE -
  KB879_RS28560 (KB879_28560) rpmA 6136708..6136968 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  KB879_RS28565 (KB879_28565) rplU 6137008..6137319 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  KB879_RS28570 (KB879_28570) ispB 6137850..6138779 (+) 930 WP_049800579.1 octaprenyl diphosphate synthase -
  KB879_RS28580 (KB879_28580) - 6139097..6139378 (-) 282 WP_249221207.1 helix-turn-helix domain-containing protein -
  KB879_RS28585 (KB879_28585) pilB 6139619..6141349 (+) 1731 WP_211980320.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KB879_RS28590 (KB879_28590) - 6141429..6142700 (+) 1272 WP_013958059.1 type II secretion system F family protein -
  KB879_RS28595 (KB879_28595) pilD 6142704..6143606 (+) 903 WP_013958060.1 A24 family peptidase Machinery gene
  KB879_RS28600 (KB879_28600) coaE 6143670..6144296 (+) 627 WP_035826779.1 dephospho-CoA kinase -
  KB879_RS28605 (KB879_28605) zapD 6144506..6145264 (+) 759 WP_035826777.1 cell division protein ZapD -
  KB879_RS28610 (KB879_28610) - 6145282..6145470 (+) 189 WP_013958063.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 63611.23 Da        Isoelectric Point: 6.8828

>NTDB_id=559878 KB879_RS28585 WP_211980320.1 6139619..6141349(+) (pilB) [Cupriavidus sp. KK10]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMNAHDLALFAADKYQLPLLDLAQYNLTKVPPALAGNREF
HAHRLLPLGRRENRLVLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLRNISPVQAERKMIEYDP
VAAAGNQRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDKDAKETVERAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCTCKREGEGEIPREA
LLEAGFREQDLDGSWQPYHPVGCERCNGTGYKGRCGIYQVMPITEAMQEIILTHGTALQIAEQARKDGVLSLREAGLLKV
KQGVTSLEEVLATTNT

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=559878 KB879_RS28585 WP_211980320.1 6139619..6141349(+) (pilB) [Cupriavidus sp. KK10]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
AAAGCAGTCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAACGCGCACGACCTGGCGCTGTTTGCCGCGGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTGACCAAGGTGCCGCCGGCGCTGGCCGGCAACCGTGAATTC
CACGCGCACCGGTTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCGATGTCCGACCCGTCCAACCAGGCCGG
GCTGGATGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCCCTGGGCACGCTCAGGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTGGCCGCGGCCGGCAACCAGCGCAACCGCACCACCGCCGACAATATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCACCGCGGCGCGTCCGACCTGCACTTCGAGCCGTTCGAGACCTTCTACCGCGTCCGCTTCC
GCGTGGACGGGGTGCTGCAGGAAGTTGCACGCCCGCCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCGCGCTTGGACATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATTGCCCTGCCCAAGGACAAGGA
CGCCAAGGAGACGGTCGAGAGGGCAGTGGATTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCTTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTAGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCCACCGCGGAAGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGCCTGACCTTTGCCGCGGCCCTGCGTTCGTTCCTGCGGCAGGACCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGTCACCTGGTGTTGTCGACGCT
GCACACCAACGATGCGCCGACCACGCTGACGCGGTTGATGAACATGGGCGTGGCGGCCTTCAATATTGCGTCGAGCGTGC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGTACCTGCAAGCGCGAGGGCGAGGGCGAGATCCCGCGCGAGGCG
CTGTTGGAGGCGGGCTTCCGCGAACAAGACCTGGACGGCAGCTGGCAGCCCTACCACCCGGTCGGCTGCGAGCGCTGCAA
CGGCACCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGACGC
ACGGCACGGCACTGCAGATCGCCGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTG
AAGCAGGGCGTCACGTCACTCGAAGAAGTGCTGGCGACAACGAATACTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.189

99.132

0.517
  pilF Neisseria gonorrhoeae MS11

51.379

100

0.517
  pilB Acinetobacter baylyi ADP1

51.885

96.701

0.502
  pilB Legionella pneumophila strain ERS1305867

49.546

95.66

0.474
  pilB Vibrio cholerae strain A1552

45.009

99.132

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

46.396

93.924

0.436
  pilB Vibrio campbellii strain DS40M4

45.856

94.271

0.432
  pilF Thermus thermophilus HB27

38.86

100

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.138

96.701

0.378