Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCG54_RS01460 Genome accession   NZ_CP073115
Coordinates   301529..303208 (-) Length   559 a.a.
NCBI ID   WP_254324430.1    Uniprot ID   A0A9X9HV71
Organism   Neisseria subflava strain TT0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 296529..308208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG54_RS01430 (KCG54_01400) lspA 296884..297390 (-) 507 WP_049331903.1 signal peptidase II -
  KCG54_RS01435 (KCG54_01405) glyQ 297539..298438 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  KCG54_RS01440 (KCG54_01410) yacG 298520..298702 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  KCG54_RS01445 (KCG54_01415) coaE 298699..299319 (-) 621 WP_254324428.1 dephospho-CoA kinase -
  KCG54_RS01450 (KCG54_01420) pilD 299319..300206 (-) 888 WP_254324429.1 A24 family peptidase Machinery gene
  KCG54_RS01455 (KCG54_01425) pilG 300208..301449 (-) 1242 WP_254322302.1 type II secretion system F family protein Machinery gene
  KCG54_RS01460 (KCG54_01430) pilF 301529..303208 (-) 1680 WP_254324430.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG54_RS01465 (KCG54_01435) carB 303611..306826 (-) 3216 WP_254324431.1 carbamoyl-phosphate synthase large subunit -
  KCG54_RS01470 (KCG54_01440) - 306852..307604 (-) 753 WP_049344526.1 hypothetical protein -
  KCG54_RS01475 (KCG54_01445) - 307882..308139 (-) 258 WP_049225703.1 SemiSWEET family transporter -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62250.53 Da        Isoelectric Point: 6.2018

>NTDB_id=558021 KCG54_RS01460 WP_254324430.1 301529..303208(-) (pilF) [Neisseria subflava strain TT0077]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPKLFSDGIISPKALGELVARVFSYPLLDLRYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKHEVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=558021 KCG54_RS01460 WP_254324430.1 301529..303208(-) (pilF) [Neisseria subflava strain TT0077]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCAAACTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTAGTCGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCGCTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAATCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTCGTAGTTCCAGACGACCAGCTCAGCTCACTGC
TGGAATGGCTAGGTCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACCCAGCAATCTCAATCG
CTTTATATCGATAACGAAGAAGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAACGC
AGGCGCATCGGACATCCACTTTGAATTCTACGAACAAATGGCGCGCGTCCGTTTCCGTGTGGACGGGCAGTTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACATTGCC
GACATTGTTCGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGTTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTATTGGTTACCGGCCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGCATTTTGAACACGGAAGATGTGAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCTGCCGCACTCCGATCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
CGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTAATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCGAACTGCAAAC
ACGAAGTTGAGCGCCCACCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAGCTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAATATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGTGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

50.88

100

0.517
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.632

96.78

0.374