Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KCX70_RS02470 Genome accession   NZ_CP073105
Coordinates   508902..511046 (+) Length   714 a.a.
NCBI ID   WP_212619181.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503902..516046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX70_RS02450 (KCX70_02450) comM 506070..507134 (+) 1065 WP_212619177.1 pilus assembly protein PilM Machinery gene
  KCX70_RS02455 (KCX70_02455) pilN 507134..507706 (+) 573 WP_212619178.1 type 4a pilus biogenesis protein PilN -
  KCX70_RS02460 (KCX70_02460) pilO 507703..508326 (+) 624 WP_212619179.1 type 4a pilus biogenesis protein PilO -
  KCX70_RS02465 (KCX70_02465) pilP 508323..508850 (+) 528 WP_212619180.1 type 4a pilus biogenesis lipoprotein PilP -
  KCX70_RS02470 (KCX70_02470) pilQ 508902..511046 (+) 2145 WP_212619181.1 type IV pilus secretin PilQ Machinery gene
  KCX70_RS02475 (KCX70_02475) aroK 511053..511598 (+) 546 WP_212619182.1 shikimate kinase AroK -
  KCX70_RS02480 (KCX70_02480) aroB 511623..512726 (+) 1104 WP_212619183.1 3-dehydroquinate synthase -
  KCX70_RS02485 (KCX70_02485) - 512736..514232 (+) 1497 WP_212619184.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 76911.15 Da        Isoelectric Point: 4.8207

>NTDB_id=557938 KCX70_RS02470 WP_212619181.1 508902..511046(+) (pilQ) [Stutzerimonas stutzeri strain F2]
MNSTLSRLGLSMLAAFISPALLAANLNSLDVASLPGDRVELKLAFDEPVPEPRGYTLDQPARIALDLPGVSSKLASKSRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGNNLFVVLGESNGAAPVAAAAPVVPVQRAAPVAPLAVGKAITNVD
FRRGEDGAGNVVISLSDASISPSIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKADGASILIEPTGRYD
YLAYQTDDKLTISIKPLSEADAEKRKAEHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALELVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDKGGESN
DRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNAWGKNGQL
GVADDPDATGKPGDDDYIPSRQVGRFPGIPEVPLNTPFVDMGAIGATSGIGLGFITNNAILDLQLSAMEKTGNGEVVSQP
KVVTADKETAKILKGAEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGVPSIRKN
EVNAKVLVTDGETIVIGGVFSNTQSKSVDKVPFLGDLPFVGRVFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=557938 KCX70_RS02470 WP_212619181.1 508902..511046(+) (pilQ) [Stutzerimonas stutzeri strain F2]
ATGAATAGCACTCTCTCTCGTCTCGGCCTCTCCATGCTGGCGGCGTTCATTTCGCCCGCCCTGTTGGCGGCAAACCTGAA
TTCACTCGATGTCGCGTCGCTGCCCGGTGATCGAGTCGAGCTGAAACTGGCGTTCGACGAGCCGGTACCGGAGCCGCGCG
GTTATACGCTCGATCAGCCGGCGCGCATCGCGCTCGACCTGCCCGGGGTGTCCAGCAAGTTGGCCTCGAAGAGCCGTGAA
CTCGGTGTGGGGAATGCGCGAAGCGTAACGGTGGTCGAGGCACAGGGGCGTACCCGGCTGATCGTGAATCTGACGTCTCT
GGTGCCATACTCCACGCGCGTCGATGGCAATAATCTGTTCGTTGTGCTTGGCGAAAGCAACGGTGCCGCTCCTGTCGCCG
CCGCTGCGCCGGTAGTTCCCGTGCAGCGCGCCGCACCGGTTGCCCCGCTGGCTGTGGGCAAAGCAATTACCAACGTCGAC
TTCCGGCGCGGCGAAGATGGTGCCGGCAATGTCGTGATTTCTCTTTCCGATGCTTCGATCAGTCCGAGCATCGAAGAGCA
AGGTGGAAAGATTCGCGTGTCCTTCGCCAAGACGCAGCTGCCGGAGTCGCTGCGTGTTCGATTGGACGTCCAGGACTTCG
CTACGCCGGTCAAGTTCGTCGACTCCAGCAGCAAGGCCGATGGTGCGAGCATCTTGATCGAGCCCACCGGGCGTTACGAC
TACCTGGCCTACCAGACCGATGACAAGCTGACCATCAGCATCAAGCCGCTCAGCGAGGCAGATGCCGAGAAGCGTAAGGC
CGAGCATTTCACCTATTCGGGGGAAAAGCTGTCGCTGAATTTCCAGGACATCGATGTTCGCTCCGTATTGCAACTGATCG
CGGACTTCACCGACCTCAACTTGGTCGCCAGCGATACCGTACAAGGCAACATCACGTTGCGCCTGCAGAACGTACCCTGG
GACCAGGCGCTGGAATTGGTGCTCAAAACCAAGGGTCTCGACAAGCGCCAGGTGGGCAATGTCCTGCTGGTCGCCCCTGC
CGACGAAATTGCCGCGCGTGAGCGGCAGGAGCTCGAGTCGCAGCGTCAGATTGCCGAGCTGGCACCCTTGCGCCGGGAAG
TCGTGCAGGTCAACTATGCCAAGGCTGCCGATATTGCCCGGTTGTTCCAGTCCGTAACCGACAAGGGCGGCGAGAGCAAC
GATCGCGGCTCGATCGCGGTTGATGACCGTACCAACAATATCATTGCCTATCAGACCCAGGAGCGCCTCGACGAGCTCCG
CCGTATCGTCGCTCAGCTGGATATTCCGGTGCGCCAGGTCATGATCGAGGCGCGGATCGTCGAAGCGAACGTCGACTATG
ACAAGAGCCTGGGCGTTCGCTGGGGTGGAAATCTCTGGGCGGGAGACAAGTGGAATGCTTGGGGTAAGAACGGGCAGCTC
GGAGTCGCTGACGATCCGGACGCAACAGGCAAGCCAGGCGATGATGACTACATTCCCTCTCGACAGGTGGGTCGCTTCCC
TGGAATTCCTGAGGTTCCGCTGAATACTCCGTTCGTCGACATGGGGGCGATAGGCGCAACGTCCGGCATAGGTCTAGGCT
TTATCACGAACAATGCCATCCTCGACTTGCAGCTCTCGGCAATGGAAAAGACCGGTAATGGCGAGGTCGTTTCCCAGCCC
AAGGTGGTTACCGCTGACAAGGAAACGGCGAAGATCCTCAAGGGCGCCGAGGTTCCGTATCAGGAGGCGAGCTCCAGCGG
CGCAACCAGCACCTCGTTCAAGGAAGCAGCACTGTCCCTGGAGGTTACGCCGCAGATCACGCCGGATAACCGCATCATCA
TGGAGGTGAAGGTGACCAAGGATGAGCCGGACTTCTCTCAGGCGGCCAGCACCGGCGGCGTTCCGTCGATTCGCAAGAAC
GAAGTTAACGCCAAGGTTCTGGTGACCGACGGCGAGACCATCGTCATCGGTGGAGTGTTCTCCAACACGCAGAGCAAGTC
GGTCGACAAGGTTCCGTTCCTGGGCGATCTGCCTTTCGTGGGACGGGTGTTCCGGCGTGACCTGGTGCAGGACAGAAAGT
CCGAACTGTTGGTATTCATCACTCCGCGGATCATGAATAATCAGGCGATCCAAGCCAGTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(547-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.414

100

0.735
  comQ Acinetobacter baylyi ADP1

40.699

100

0.408
  pilQ Acinetobacter baumannii strain A118

38.309

100

0.406
  pilQ Acinetobacter baumannii D1279779

38.309

100

0.406
  pilQ Legionella pneumophila strain ERS1305867

37.283

96.919

0.361