Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KAQ61_RS00740 Genome accession   NZ_CP072916
Coordinates   164252..165997 (-) Length   581 a.a.
NCBI ID   WP_210930174.1    Uniprot ID   A0AA42HP60
Organism   Comamonas aquatica strain NEB418     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 159252..170997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAQ61_RS00710 (KAQ61_00710) - 159846..160307 (+) 462 WP_210930171.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  KAQ61_RS00715 (KAQ61_00715) - 160411..160620 (-) 210 WP_045267488.1 DNA gyrase inhibitor YacG -
  KAQ61_RS00720 (KAQ61_00720) zapD 160647..161402 (-) 756 WP_210930172.1 cell division protein ZapD -
  KAQ61_RS00725 (KAQ61_00725) coaE 161497..162105 (-) 609 WP_154163335.1 dephospho-CoA kinase -
  KAQ61_RS00730 (KAQ61_00730) pilD 162108..162977 (-) 870 WP_210930173.1 prepilin peptidase Machinery gene
  KAQ61_RS00735 (KAQ61_00735) pilC 162977..164200 (-) 1224 WP_045267485.1 type II secretion system F family protein Machinery gene
  KAQ61_RS00740 (KAQ61_00740) pilB 164252..165997 (-) 1746 WP_210930174.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KAQ61_RS00745 (KAQ61_00745) - 166156..167085 (-) 930 WP_210931650.1 polyprenyl synthetase family protein -
  KAQ61_RS00750 (KAQ61_00750) rplU 167296..167607 (+) 312 WP_042417032.1 50S ribosomal protein L21 -
  KAQ61_RS00755 (KAQ61_00755) rpmA 167626..167886 (+) 261 WP_042416998.1 50S ribosomal protein L27 -
  KAQ61_RS00760 (KAQ61_00760) cgtA 168050..169123 (+) 1074 WP_042416995.1 Obg family GTPase CgtA -
  KAQ61_RS00765 (KAQ61_00765) proB 169204..170340 (+) 1137 WP_042416992.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63313.63 Da        Isoelectric Point: 6.3015

>NTDB_id=556862 KAQ61_RS00740 WP_210930174.1 164252..165997(-) (pilB) [Comamonas aquatica strain NEB418]
MNAASTASLIAQSSSNTTLAGLARALVSAGQLPEAVALETARKAHASKNSFVAQLVASGHMPAAELAFQIAQIFRTPLLD
LDTIDPQRLPRDLLDAKLCSMHKVLPLLKRGNRLVVATADPTDKDAAEKIKFTAQMGVDWVIVECDKLQRLVEATTKSAS
EALDAFNSDADFQFDEVVDAPAPDDKDSNASEVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHSYRVRFRIDGELREIA
SPPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPE
EKGRLLDAIQRPYGMILVTGPTGSGKTVSLYSCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFATALRSFLR
QDPDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPH
DVPHETLVEAGYEEHQLNGSWVPYKAVGCSACNNGYKGRVGIYQVMPITEAIQQIILRDGSALEIAAQARSEGVKSLRES
GLDKVRQGITSLEEVLAVTNL

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=556862 KAQ61_RS00740 WP_210930174.1 164252..165997(-) (pilB) [Comamonas aquatica strain NEB418]
ATGAACGCCGCATCCACTGCATCCTTGATCGCCCAGTCCTCCTCGAACACGACACTGGCCGGGCTGGCCCGGGCCCTGGT
CAGCGCAGGCCAGCTCCCGGAAGCGGTTGCGCTGGAGACGGCCCGCAAGGCACACGCCAGCAAGAACAGCTTCGTGGCAC
AGCTGGTGGCATCCGGGCACATGCCTGCGGCGGAGCTGGCCTTTCAGATCGCACAGATCTTTCGTACACCGCTGCTGGAC
CTGGACACCATCGATCCCCAGCGCCTGCCCAGGGATTTGCTGGACGCCAAGCTGTGCAGCATGCACAAGGTGCTGCCCCT
GCTCAAGCGCGGCAACCGCCTGGTGGTGGCCACAGCAGACCCCACCGACAAGGACGCCGCGGAGAAGATCAAATTCACCG
CCCAGATGGGGGTGGACTGGGTCATCGTCGAGTGCGACAAACTGCAGCGGCTGGTCGAAGCCACCACCAAGAGTGCCTCT
GAGGCCCTGGACGCTTTCAATTCCGATGCCGACTTCCAATTCGACGAAGTGGTGGATGCGCCCGCCCCGGACGACAAGGA
CAGCAATGCATCCGAGGTCGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATTGACGCGTTCAACATGCGGG
CCTCGGACCTGCACTTTGAGCCCTACGAACACAGCTACCGCGTGCGCTTCCGGATCGATGGCGAACTGCGGGAGATCGCA
AGCCCGCCCATCGCCATCAAGGAAAAACTGGCCTCGCGCATCAAGGTGATCTCGCGACTGGACATCTCGGAAAAACGCGT
TCCCCAGGATGGGCGCATGAAGCTCAAGGTCGGTGCTGACCGCGTCATCGACTTCCGTGTGTCCACCTTGCCCACGCTGT
TCGGCGAGAAGATCGTGATCCGTATCCTGGACCCCAGCAGCGCCAGGATGGGCATTGATGCCCTTGGCTACGAGCCCGAG
GAAAAGGGTCGTCTGCTGGATGCCATCCAGCGGCCCTACGGCATGATCCTGGTCACAGGGCCCACCGGCTCGGGCAAGAC
CGTGTCGCTATACAGCTGCCTGAACCTGCTGAACAAACCCGGGGTCAACATCTCCACGGCCGAAGATCCCTCGGAAATCA
ACATGCCCGGCGTCAACCAGGTCAATGTGAATGAAAAGGCCGGACTGACTTTCGCCACCGCCCTGCGATCCTTCCTGCGC
CAGGACCCGGATGTCATCATGGTCGGTGAAATCCGTGATCTGGAAACAGCCGACATCGCCATCAAGGCCGCGCAGACGGG
GCACTTGGTGCTATCCACCCTGCACACCAACGATGCGCCCACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCGT
TCAACATCGCCTCCAGCGTGATCCTGATCACGGCCCAGCGTCTGGCGCGCCGCTTGTGCCCACAATGCAAGCAGCCGCAT
GATGTGCCACACGAAACCCTGGTCGAAGCCGGTTACGAGGAACACCAGCTGAATGGCTCCTGGGTCCCCTACAAGGCTGT
GGGCTGCAGTGCCTGCAACAATGGTTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCACCGAAGCAATCCAAC
AAATCATCCTGCGCGACGGCAGTGCCCTGGAGATTGCCGCCCAGGCCAGGAGCGAAGGCGTGAAATCGCTGCGCGAATCC
GGATTGGACAAAGTCCGGCAAGGCATCACTTCGCTGGAAGAGGTGCTGGCCGTCACCAATTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-155)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.3

97.418

0.539
  pilB Acinetobacter baylyi ADP1

55.496

97.074

0.539
  pilF Neisseria gonorrhoeae MS11

51.865

96.902

0.503
  pilB Legionella pneumophila strain ERS1305867

50.436

98.623

0.497
  pilB Vibrio cholerae strain A1552

47.197

95.181

0.449
  pilB Vibrio campbellii strain DS40M4

45.633

96.558

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

46.927

92.427

0.434
  pilF Thermus thermophilus HB27

40.278

99.139

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

91.738

0.363