Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   J9537_RS08040 Genome accession   NZ_CP072888
Coordinates   1634637..1635242 (-) Length   201 a.a.
NCBI ID   WP_218255588.1    Uniprot ID   -
Organism   Enterococcus raffinosus strain F162_2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629637..1640242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9537_RS08025 (J9537_08025) - 1630207..1632294 (-) 2088 WP_245001247.1 DNA internalization-related competence protein ComEC/Rec2 -
  J9537_RS08030 (J9537_08030) - 1632432..1632923 (-) 492 WP_218255586.1 ComE operon protein 2 -
  J9537_RS08035 (J9537_08035) - 1633292..1634476 (-) 1185 WP_218255587.1 MFS transporter -
  J9537_RS08040 (J9537_08040) comEA 1634637..1635242 (-) 606 WP_218255588.1 helix-hairpin-helix domain-containing protein Machinery gene
  J9537_RS08045 (J9537_08045) sepM 1635335..1636378 (-) 1044 WP_218257349.1 SepM family pheromone-processing serine protease Regulator
  J9537_RS08050 (J9537_08050) coaD 1636368..1636862 (-) 495 WP_010744912.1 pantetheine-phosphate adenylyltransferase -
  J9537_RS08055 (J9537_08055) rsmD 1636864..1637418 (-) 555 WP_218255589.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  J9537_RS08060 (J9537_08060) - 1637578..1637925 (-) 348 WP_028020682.1 YlbG family protein -
  J9537_RS08065 (J9537_08065) - 1637912..1638337 (-) 426 WP_218255590.1 YlbF family regulator -
  J9537_RS08070 (J9537_08070) - 1638339..1639445 (-) 1107 WP_218255591.1 CAP-associated domain-containing protein -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 22350.27 Da        Isoelectric Point: 4.4221

>NTDB_id=556641 J9537_RS08040 WP_218255588.1 1634637..1635242(-) (comEA) [Enterococcus raffinosus strain F162_2]
MKEYLKKYPYWLAVPAILAVILFFFFSRTSEEELTDPAVFSSESSRVETSESINQEWYVDVKGAVKKTGMYRISKGMRLM
DAIDLAGGFTAEADQNQINFSKLLSDQEIIYVPKVGEEIPRLKEATVESDGSIGGESQEKININTADATELQKLSGIGEK
KAADIIKYREENGSFNAIDDLTKVSGIGEKTLENLKDSITI

Nucleotide


Download         Length: 606 bp        

>NTDB_id=556641 J9537_RS08040 WP_218255588.1 1634637..1635242(-) (comEA) [Enterococcus raffinosus strain F162_2]
ATGAAAGAGTATTTAAAAAAGTATCCTTACTGGCTAGCAGTACCGGCTATTCTTGCAGTTATTCTTTTTTTCTTCTTTTC
CAGAACATCTGAAGAAGAGCTAACAGATCCAGCCGTTTTTTCTTCAGAGAGCAGTAGGGTAGAGACAAGTGAATCTATCA
ATCAAGAGTGGTATGTCGATGTCAAAGGAGCGGTCAAAAAAACAGGAATGTATCGTATTTCAAAAGGTATGCGTTTAATG
GATGCTATTGATCTTGCAGGTGGGTTTACAGCTGAGGCGGATCAGAATCAGATCAATTTTTCAAAGCTTTTGTCGGACCA
AGAAATCATTTATGTACCTAAAGTTGGAGAGGAAATTCCTAGATTAAAAGAAGCAACAGTAGAGTCAGATGGATCAATTG
GAGGGGAATCTCAAGAAAAGATCAACATCAACACAGCAGATGCGACAGAATTGCAGAAATTATCAGGAATTGGTGAGAAA
AAGGCGGCGGATATAATCAAATATCGAGAGGAAAATGGCAGCTTCAACGCGATCGATGACCTGACAAAAGTATCCGGCAT
TGGTGAGAAAACGCTTGAAAATTTAAAGGACTCAATTACAATATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(58-112)

Helix-hairpin-helix motif (HHH_3)

(137-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

48.325

100

0.502
  comEA Streptococcus thermophilus LMD-9

37.391

100

0.428
  comEA Bacillus subtilis subsp. subtilis str. 168

40

100

0.408
  comEA Latilactobacillus sakei subsp. sakei 23K

34.632

100

0.398
  comEA Staphylococcus aureus MW2

33.636

100

0.368
  comEA Staphylococcus aureus N315

33.182

100

0.363