Detailed information NTG0556116

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J9B93_RS12210
• Genome accession: NZ_CP072843
• Coordinates: 2535816..2536430 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain VY03
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2530816..2541430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9B93_RS12180 (J9B93_12180)	rpsT	2531116..2531382 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J9B93_RS12185 (J9B93_12185)	holA	2531399..2532440 (-)	1042	Protein_2371	DNA polymerase III subunit delta	-
J9B93_RS12190 (J9B93_12190)	-	2532480..2532634 (-)	155	Protein_2372	hypothetical protein	-
J9B93_RS12195 (J9B93_12195)	-	2532675..2532809 (+)	135	WP_003152870.1	YqzM family protein	-
J9B93_RS12200 (J9B93_12200)	comEC	2532828..2535179 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J9B93_RS12205 (J9B93_12205)	-	2535180..2535749 (-)	570	WP_003152868.1	ComE operon protein 2	-
J9B93_RS12210 (J9B93_12210)	comEA	2535816..2536430 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
J9B93_RS12215 (J9B93_12215)	comER	2536489..2537310 (+)	822	WP_014305449.1	late competence protein ComER	-
J9B93_RS12220 (J9B93_12220)	-	2537379..2538116 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
J9B93_RS12225 (J9B93_12225)	rsfS	2538113..2538469 (-)	357	WP_003152864.1	ribosome silencing factor	-
J9B93_RS12230 (J9B93_12230)	yqeK	2538466..2539047 (-)	582	WP_210601933.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J9B93_RS12235 (J9B93_12235)	-	2539037..2539606 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
J9B93_RS12240 (J9B93_12240)	yhbY	2539617..2539907 (-)	291	WP_209387447.1	ribosome assembly RNA-binding protein YhbY	-
J9B93_RS12245 (J9B93_12245)	aroE	2539901..2540743 (-)	843	WP_115996555.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=556116 J9B93_RS12210 WP_041482377.1 2535816..2536430(-) (comEA) [Bacillus velezensis strain VY03]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=556116 J9B93_RS12210 WP_041482377.1 2535816..2536430(-) (comEA) [Bacillus velezensis strain VY03]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363