Detailed information NTG0555847

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J8615_RS12965
• Genome accession: NZ_CP072791
• Coordinates: 2639619..2640233 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GS-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2634619..2645233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8615_RS12935 (J8615_12925)	rpsT	2634919..2635185 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J8615_RS12940 (J8615_12930)	holA	2635202..2636243 (-)	1042	Protein_2528	DNA polymerase III subunit delta	-
J8615_RS12945 (J8615_12935)	-	2636283..2636437 (-)	155	Protein_2529	hypothetical protein	-
J8615_RS12950 (J8615_12940)	-	2636478..2636612 (+)	135	WP_003152870.1	YqzM family protein	-
J8615_RS12955 (J8615_12945)	comEC	2636631..2638982 (-)	2352	WP_046559887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J8615_RS12960 (J8615_12950)	-	2638983..2639552 (-)	570	WP_003152868.1	ComE operon protein 2	-
J8615_RS12965 (J8615_12955)	comEA	2639619..2640233 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
J8615_RS12970 (J8615_12960)	comER	2640292..2641113 (+)	822	WP_014305449.1	late competence protein ComER	-
J8615_RS12975 (J8615_12965)	-	2641182..2641919 (-)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
J8615_RS12980 (J8615_12970)	rsfS	2641916..2642272 (-)	357	WP_003152864.1	ribosome silencing factor	-
J8615_RS12985 (J8615_12975)	yqeK	2642290..2642850 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J8615_RS12990 (J8615_12980)	-	2642840..2643409 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
J8615_RS12995 (J8615_12985)	yhbY	2643420..2643710 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
J8615_RS13000 (J8615_12990)	aroE	2643704..2644546 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=555847 J8615_RS12965 WP_041482377.1 2639619..2640233(-) (comEA) [Bacillus velezensis strain GS-1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=555847 J8615_RS12965 WP_041482377.1 2639619..2640233(-) (comEA) [Bacillus velezensis strain GS-1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363