Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J9247_RS27740 Genome accession   NZ_CP072783
Coordinates   5953497..5955641 (-) Length   714 a.a.
NCBI ID   WP_121358471.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LICME WGH-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5948497..5960641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9247_RS27725 (J9247_27725) - 5950131..5951804 (-) 1674 WP_128667301.1 AAA family ATPase -
  J9247_RS27730 (J9247_27730) aroB 5951811..5952917 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  J9247_RS27735 (J9247_27735) aroK 5952967..5953485 (-) 519 WP_003095833.1 shikimate kinase AroK -
  J9247_RS27740 (J9247_27740) pilQ 5953497..5955641 (-) 2145 WP_121358471.1 type 4a pilus secretin PilQ Machinery gene
  J9247_RS27745 (J9247_27745) pilP 5955695..5956219 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  J9247_RS27750 (J9247_27750) pilO 5956216..5956839 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  J9247_RS27755 (J9247_27755) pilN 5956836..5957432 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  J9247_RS27760 (J9247_27760) pilM 5957432..5958496 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77438.99 Da        Isoelectric Point: 5.2307

>NTDB_id=555678 J9247_RS27740 WP_121358471.1 5953497..5955641(-) (pilQ) [Pseudomonas aeruginosa strain LICME WGH-6]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SEGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGVKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=555678 J9247_RS27740 WP_121358471.1 5953497..5955641(-) (pilQ) [Pseudomonas aeruginosa strain LICME WGH-6]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCTAAGGATCGCACACGGCTGATCATCAACCTGACCACGTT
GTCGTCCTATACCACCCGCGTGGAAGGCAACAATCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCCTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGTCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGATGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTGAACTTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGTCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGAGGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAGCTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CGCGCATCGTCGAGGCCAACGTCGGCTACGACAAGAGCCTCGGGGTGCGCTGGGGCGGCGCCTACCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGGTCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCCACCTCGGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGATGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTGCCGTTCCTCGGTGAACTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCTATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.72

100

0.997
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.408
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.408
  comQ Acinetobacter baylyi ADP1

41.168

98.319

0.405
  pilQ Legionella pneumophila strain Lp02

38.102

95.938

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

95.938

0.364