Detailed information NTG0552753

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: J7U00_RS05555
• Genome accession: NZ_CP072431
• Coordinates: 1061491..1062330 (-)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus strain S24745
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1056491..1067330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7U00_RS05540 (J7U00_05545)	-	1057543..1058202 (-)	660	WP_011227158.1	response regulator transcription factor	-
J7U00_RS05545 (J7U00_05550)	-	1058238..1059062 (-)	825	WP_011681087.1	TraX family protein	-
J7U00_RS05550 (J7U00_05555)	topA	1059179..1061323 (-)	2145	WP_011681086.1	type I DNA topoisomerase	-
J7U00_RS05555 (J7U00_05560)	dprA	1061491..1062330 (-)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
J7U00_RS05560 (J7U00_05565)	-	1062398..1063318 (-)	921	WP_138496622.1	ROK family protein	-
J7U00_RS05565 (J7U00_05570)	-	1063329..1064096 (-)	768	WP_002950603.1	ribonuclease HII	-
J7U00_RS05570 (J7U00_05575)	ylqF	1064083..1064937 (-)	855	WP_138496624.1	ribosome biogenesis GTPase YlqF	-
J7U00_RS05575 (J7U00_05580)	-	1065065..1065754 (-)	690	WP_046206437.1	helix-turn-helix transcriptional regulator	-
J7U00_RS05580 (J7U00_05585)	-	1065849..1066364 (-)	516	WP_084828772.1	transcription repressor NadR	-
J7U00_RS05585 (J7U00_05590)	-	1066388..1066915 (-)	528	WP_041827017.1	ECF transporter S component	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=552753 J7U00_RS05555 WP_011225887.1 1061491..1062330(-) (dprA) [Streptococcus thermophilus strain S24745]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=552753 J7U00_RS05555 WP_011225887.1 1061491..1062330(-) (dprA) [Streptococcus thermophilus strain S24745]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCTTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCACCACCTCATTCAAGAGGGTGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362