Detailed information NTG0551909

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J6B42_RS12650
• Genome accession: NZ_CP072311
• Coordinates: 2575524..2576138 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SC60
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2570524..2581138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J6B42_RS12620 (J6B42_12550)	rpsT	2570824..2571090 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J6B42_RS12625 (J6B42_12555)	holA	2571107..2572148 (-)	1042	Protein_2445	DNA polymerase III subunit delta	-
J6B42_RS12630 (J6B42_12560)	-	2572188..2572342 (-)	155	Protein_2446	hypothetical protein	-
J6B42_RS12635 (J6B42_12565)	-	2572383..2572517 (+)	135	WP_003152870.1	YqzM family protein	-
J6B42_RS12640 (J6B42_12570)	comEC	2572536..2574887 (-)	2352	WP_025852986.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J6B42_RS12645 (J6B42_12575)	-	2574888..2575457 (-)	570	WP_003152868.1	ComE operon protein 2	-
J6B42_RS12650 (J6B42_12580)	comEA	2575524..2576138 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
J6B42_RS12655 (J6B42_12585)	comER	2576197..2577018 (+)	822	WP_003152866.1	late competence protein ComER	-
J6B42_RS12660 (J6B42_12590)	-	2577087..2577824 (-)	738	WP_025852989.1	class I SAM-dependent methyltransferase	-
J6B42_RS12665 (J6B42_12595)	rsfS	2577821..2578177 (-)	357	WP_003152864.1	ribosome silencing factor	-
J6B42_RS12670 (J6B42_12600)	yqeK	2578174..2578755 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J6B42_RS12675 (J6B42_12605)	-	2578745..2579314 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
J6B42_RS12680 (J6B42_12610)	yhbY	2579325..2579615 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
J6B42_RS12685 (J6B42_12615)	aroE	2579609..2580451 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=551909 J6B42_RS12650 WP_003152867.1 2575524..2576138(-) (comEA) [Bacillus velezensis strain SC60]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=551909 J6B42_RS12650 WP_003152867.1 2575524..2576138(-) (comEA) [Bacillus velezensis strain SC60]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363