Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XeaCFBP3836p_RS15650 Genome accession   NZ_CP072268
Coordinates   3671959..3673695 (-) Length   578 a.a.
NCBI ID   WP_014090854.1    Uniprot ID   -
Organism   Xanthomonas euvesicatoria pv. alfalfae strain CFBP3836     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3666959..3678695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XeaCFBP3836p_RS15635 (XeaCFBP3836p_15635) sucC 3667002..3668171 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  XeaCFBP3836p_RS15640 (XeaCFBP3836p_15640) - 3668403..3670016 (+) 1614 WP_029820065.1 HAMP domain-containing sensor histidine kinase -
  XeaCFBP3836p_RS15645 (XeaCFBP3836p_15645) pilR 3670284..3671744 (+) 1461 WP_029820066.1 sigma-54 dependent transcriptional regulator Regulator
  XeaCFBP3836p_RS15650 (XeaCFBP3836p_15650) pilB 3671959..3673695 (-) 1737 WP_014090854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XeaCFBP3836p_RS15655 (XeaCFBP3836p_15655) - 3673738..3674148 (-) 411 WP_029820067.1 pilin -
  XeaCFBP3836p_RS15660 (XeaCFBP3836p_15660) pilA 3674333..3674743 (-) 411 WP_029820068.1 pilin Machinery gene
  XeaCFBP3836p_RS15665 (XeaCFBP3836p_15665) pilC 3675077..3676333 (+) 1257 WP_014090856.1 type II secretion system F family protein Machinery gene
  XeaCFBP3836p_RS15670 (XeaCFBP3836p_15670) - 3676340..3677203 (+) 864 WP_008576868.1 A24 family peptidase -
  XeaCFBP3836p_RS15675 (XeaCFBP3836p_15675) coaE 3677217..3677822 (+) 606 WP_029820069.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62535.58 Da        Isoelectric Point: 5.6006

>NTDB_id=551393 XeaCFBP3836p_RS15650 WP_014090854.1 3671959..3673695(-) (pilB) [Xanthomonas euvesicatoria pv. alfalfae strain CFBP3836]
MNAVSSANLVGITGIARRLVQDGAIEEPAARAAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=551393 XeaCFBP3836p_RS15650 WP_014090854.1 3671959..3673695(-) (pilB) [Xanthomonas euvesicatoria pv. alfalfae strain CFBP3836]
ATGAATGCTGTTTCGTCCGCTAATTTGGTTGGCATCACGGGAATTGCCCGTCGTTTAGTACAAGATGGCGCGATTGAAGA
GCCTGCTGCTCGGGCTGCGATGGATCAGGCAGCGGCTGCGAAGGTTCCATTGCCCCAATGGTTTTCGGAAAAAAAGCTGG
TATCGGCGTCGCAACTGGCTGCTGCCAATGCAGTCGAGTTCGGTATGCCCCTGATGGATGTGTCGGCATTCGACGCAAGC
CAGAACGCAGTAAAGCTCGTCAGCGAAGAGTTGCTCCAGAAGCACCAAGTGTTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTGGGGGTGAGCAACCCAACCCAGACTCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.27

0.555
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.393

99.827

0.493
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

96.886

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446