Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSP22_RS22715 Genome accession   NZ_CP089064
Coordinates   4862974..4864482 (+) Length   502 a.a.
NCBI ID   WP_003158838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain UNC_PaerCF35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4857974..4869482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP22_RS22695 (LSP22_22695) - 4858004..4859215 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  LSP22_RS22700 (LSP22_22700) - 4859212..4859979 (+) 768 WP_003091381.1 SDR family oxidoreductase -
  LSP22_RS22705 (LSP22_22705) xcpQ 4860066..4862042 (-) 1977 WP_003103536.1 GspD family T2SS secretin variant XcpQ -
  LSP22_RS22710 (LSP22_22710) xcpP 4862047..4862754 (-) 708 WP_003119750.1 type II secretion system protein N -
  LSP22_RS22715 (LSP22_22715) pilB 4862974..4864482 (+) 1509 WP_003158838.1 GspE family T2SS ATPase variant XcpR Machinery gene
  LSP22_RS22720 (LSP22_22720) xcpS 4864482..4865699 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  LSP22_RS22725 (LSP22_22725) xcpT 4865704..4866150 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  LSP22_RS22730 (LSP22_22730) xcpU 4866157..4866675 (+) 519 WP_061362628.1 GspH family T2SS minor pseudopilin variant XcpU -
  LSP22_RS22735 (LSP22_22735) xcpV 4866672..4867061 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  LSP22_RS22740 (LSP22_22740) xcpW 4867058..4867771 (+) 714 WP_126895820.1 GspJ family T2SS minor pseudopilin variant XcpW -
  LSP22_RS22745 (LSP22_22745) xcpX 4867768..4868769 (+) 1002 WP_003103524.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55552.74 Da        Isoelectric Point: 6.2074

>NTDB_id=548491 LSP22_RS22715 WP_003158838.1 4862974..4864482(+) (pilB) [Pseudomonas aeruginosa strain UNC_PaerCF35]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLRALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=548491 LSP22_RS22715 WP_003158838.1 4862974..4864482(+) (pilB) [Pseudomonas aeruginosa strain UNC_PaerCF35]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAGGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGTAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGTGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGAGGCCTGCGGGCG
ATCCTCCGGCAAGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGTGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGGGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAGCTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.737

97.012

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.949

93.825

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.146

88.645

0.382
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367