Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J4N45_RS02185 Genome accession   NZ_CP071850
Coordinates   409795..411486 (-) Length   563 a.a.
NCBI ID   WP_252013345.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 404795..416486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N45_RS02155 (J4N45_02150) rplS 405422..405775 (+) 354 WP_006074418.1 50S ribosomal protein L19 -
  J4N45_RS02160 (J4N45_02155) yacG 406030..406224 (-) 195 WP_128647938.1 DNA gyrase inhibitor YacG -
  J4N45_RS02165 (J4N45_02160) zapD 406261..407001 (-) 741 WP_234494579.1 cell division protein ZapD -
  J4N45_RS02170 (J4N45_02165) coaE 407033..407641 (-) 609 WP_252013341.1 dephospho-CoA kinase -
  J4N45_RS02175 (J4N45_02170) pilD 407641..408513 (-) 873 WP_252013343.1 A24 family peptidase Machinery gene
  J4N45_RS02180 (J4N45_02175) pilC 408556..409782 (-) 1227 WP_252014637.1 type II secretion system F family protein Machinery gene
  J4N45_RS02185 (J4N45_02180) pilB 409795..411486 (-) 1692 WP_252013345.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4N45_RS02190 (J4N45_02185) - 411489..411920 (-) 432 WP_252013347.1 pilin -
  J4N45_RS02195 (J4N45_02190) nadC 412182..413072 (-) 891 WP_234494573.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J4N45_RS02200 (J4N45_02195) ampD 413207..413788 (+) 582 WP_252013350.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J4N45_RS02205 (J4N45_02200) pdhR 414199..414966 (+) 768 WP_042475867.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62358.64 Da        Isoelectric Point: 6.0082

>NTDB_id=548317 J4N45_RS02185 WP_252013345.1 409795..411486(-) (pilB) [Vibrio sp. SCSIO 43140]
MSISRLPSVLAEAQIISESQKDRLVECTQSSMESVPSIIVELGWLTAQDLTSALEKLFALPAKQLHDYDYIELCRTAGLR
ELIVKNHAIPLKQSSHTLSIAISDPSIVELENDFRFATGLQIELVICDCLDITSAIKRLYGENHKGINPSFRELTSEALS
NLVPISDSELENVEDLSQDDSPVSRFIHQVLTDAVRKKASDIHFEPYEHSYRIRLRCDGILIETHQPATQLSRRLAARIK
ILAKLDIAERRLPQDGRLKLKLSEAASIDMRVSTLPTVWGEKIVLRLLDADATQLNIDQLGYTEQQKSLYLTALKKPQGM
ILMTGPTGSGKTVSLYTGLSILNTAERNISTAEDPVEIHLEGINQVQINPKIGFDFSTALRSFLRQDPDIVMLGEIRDIE
TAEIAVKAAQTGHLVLSTLHTNSTTETVIRLKNMGVDAINLSSSLSLIIAQRLARKLCECCKIPDPMSPQLRHSLNIPNE
SIVYLANEQGCNHCNHGYLGRTGIYEVMAFTHELASAIVASASLNEIEAIAKAQGMKTLKQSGVEKLIAGITSYSELQRV
LYF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=548317 J4N45_RS02185 WP_252013345.1 409795..411486(-) (pilB) [Vibrio sp. SCSIO 43140]
TTGAGCATAAGTAGGCTACCTAGCGTTCTTGCTGAAGCTCAAATTATTTCAGAGAGTCAAAAAGATCGTCTAGTAGAATG
CACGCAATCAAGTATGGAGTCTGTTCCATCAATCATTGTGGAATTGGGCTGGCTGACAGCCCAAGACTTAACTTCGGCTT
TAGAGAAACTATTTGCTCTGCCCGCTAAACAACTGCATGACTACGACTACATAGAGCTGTGTCGAACAGCAGGACTACGC
GAGCTAATTGTAAAAAACCACGCAATTCCGCTCAAGCAAAGTAGCCACACCCTCTCTATCGCAATATCCGACCCGAGCAT
TGTCGAGTTAGAAAACGATTTTCGTTTTGCGACCGGACTACAGATAGAGCTCGTCATCTGTGATTGCCTTGATATCACAT
CGGCCATTAAGCGTCTTTATGGCGAAAACCATAAGGGCATCAATCCTAGCTTTAGAGAGCTGACTTCTGAAGCACTAAGC
AACCTAGTCCCAATCAGCGATTCGGAGCTCGAGAACGTCGAAGACCTCTCCCAAGATGACTCTCCTGTAAGTCGCTTTAT
TCATCAGGTATTGACGGATGCCGTGCGCAAAAAAGCCTCGGATATTCATTTCGAACCTTATGAGCACTCTTATCGAATAC
GGCTACGCTGTGATGGCATATTGATAGAGACTCACCAGCCTGCGACGCAGCTTAGTCGTCGATTGGCAGCTCGGATTAAG
ATCTTGGCTAAACTTGATATCGCCGAAAGGCGCTTACCGCAAGACGGTCGACTGAAACTGAAACTCTCTGAAGCAGCCTC
TATCGATATGCGCGTTTCAACCCTACCCACAGTGTGGGGTGAGAAAATCGTGTTAAGACTTTTGGACGCCGATGCCACCC
AGCTCAACATTGATCAGCTAGGTTATACCGAGCAACAAAAATCGCTTTACCTCACCGCACTAAAAAAGCCCCAAGGTATG
ATCCTAATGACAGGCCCCACTGGCAGTGGAAAAACCGTTTCGCTATACACTGGACTTAGCATTCTCAATACCGCAGAGCG
TAATATTTCTACTGCTGAAGATCCGGTTGAGATTCATCTTGAGGGCATCAATCAAGTTCAGATCAATCCAAAGATCGGCT
TTGATTTCTCTACCGCCCTTCGATCTTTTTTAAGACAAGATCCCGATATTGTCATGCTCGGCGAAATCCGAGATATCGAA
ACCGCCGAAATTGCAGTCAAGGCCGCTCAAACGGGCCACCTTGTGTTATCTACACTGCACACCAACTCGACCACAGAGAC
CGTCATTAGGCTCAAAAACATGGGTGTCGACGCTATCAACCTGTCGTCCTCTCTATCGTTGATCATTGCTCAGCGCTTAG
CACGAAAGCTGTGTGAGTGTTGTAAGATACCAGACCCAATGTCCCCTCAACTTCGTCACTCCCTCAACATTCCCAATGAG
TCGATAGTCTACCTCGCCAACGAGCAAGGCTGTAATCATTGCAATCATGGTTATCTCGGAAGGACCGGCATCTATGAGGT
AATGGCGTTTACCCATGAACTCGCCAGCGCGATAGTTGCTAGCGCAAGCCTAAATGAGATAGAAGCTATTGCTAAGGCTC
AGGGAATGAAGACGCTAAAGCAATCTGGTGTGGAAAAGCTAATCGCTGGTATTACTAGCTACAGCGAGCTGCAACGCGTG
CTCTACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-142)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.769

99.822

0.647
  pilB Vibrio campbellii strain DS40M4

61.497

99.645

0.613
  pilB Vibrio parahaemolyticus RIMD 2210633

61.429

99.467

0.611
  pilB Acinetobacter baumannii D1279779

45.422

98.934

0.449
  pilB Acinetobacter baylyi ADP1

44.268

100

0.446
  pilB Legionella pneumophila strain ERS1305867

49.693

86.856

0.432
  pilF Neisseria gonorrhoeae MS11

42.553

100

0.426