Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J4G45_RS02535 Genome accession   NZ_CP071770
Coordinates   356272..356751 (+) Length   159 a.a.
NCBI ID   WP_420830717.1    Uniprot ID   -
Organism   Acinetobacter towneri strain GX5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351272..361751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G45_RS02515 (J4G45_02515) - 351626..353047 (+) 1422 WP_207973780.1 FAD-dependent oxidoreductase -
  J4G45_RS02520 (J4G45_02520) - 353339..353929 (+) 591 WP_019836149.1 LemA family protein -
  J4G45_RS02525 (J4G45_02525) - 353949..355052 (+) 1104 WP_207973779.1 TPM domain-containing protein -
  J4G45_RS02530 (J4G45_02530) - 355046..355606 (+) 561 WP_207973778.1 TPM domain-containing protein -
  J4G45_RS02535 (J4G45_02535) pilA 356272..356751 (+) 480 WP_420830717.1 pilin Machinery gene
  J4G45_RS02540 (J4G45_02540) - 356822..357163 (+) 342 WP_207973938.1 pilin -
  J4G45_RS02545 (J4G45_02545) tfpZ 357288..358028 (+) 741 WP_207973776.1 TfpX/TfpZ family type IV pilin accessory protein -
  J4G45_RS02550 (J4G45_02550) - 358182..359813 (+) 1632 WP_207973775.1 PglL family O-oligosaccharyltransferase -
  J4G45_RS02555 (J4G45_02555) - 360032..360274 (+) 243 WP_019836164.1 type II toxin-antitoxin system ParD family antitoxin -
  J4G45_RS02560 (J4G45_02560) - 360267..360560 (+) 294 WP_179989812.1 type II toxin-antitoxin system RelE/ParE family toxin -
  J4G45_RS02565 (J4G45_02565) bfr 360732..361196 (-) 465 WP_131260631.1 heteropolymeric bacterioferritin subunit Bfr -
  J4G45_RS02570 (J4G45_02570) - 361444..361638 (-) 195 WP_004974511.1 bacterioferritin-associated ferredoxin -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 15872.21 Da        Isoelectric Point: 7.7765

>NTDB_id=547616 J4G45_RS02535 WP_420830717.1 356272..356751(+) (pilA) [Acinetobacter towneri strain GX5]
MQKGFTLIELMIVVAIIGILAAIAIPAYQDYTKRAKVTEGLSLAASAKTAVTENASNGVAYGLGWTAPATTANVGTITIS
GTTGAISIPYSTSIAAATENTLVLIPFTGAGTALPDSSSAYTPPADAIQWQCAAAGATPKVTGATVGTLPAKLAPSNCR

Nucleotide


Download         Length: 480 bp        

>NTDB_id=547616 J4G45_RS02535 WP_420830717.1 356272..356751(+) (pilA) [Acinetobacter towneri strain GX5]
ATGCAAAAAGGTTTTACCCTTATCGAATTAATGATCGTTGTGGCGATCATTGGTATTTTGGCTGCAATTGCGATTCCTGC
TTATCAGGATTACACTAAACGTGCAAAAGTGACTGAGGGCTTGTCATTGGCAGCTAGCGCTAAAACTGCTGTAACAGAAA
ATGCTTCAAATGGTGTGGCTTATGGTTTAGGTTGGACTGCTCCAGCAACAACAGCTAATGTTGGCACAATTACAATCAGC
GGTACTACGGGTGCAATTTCAATTCCGTATTCTACTTCTATTGCTGCTGCTACAGAAAATACATTGGTATTAATTCCATT
TACTGGTGCAGGTACAGCTTTACCAGATAGTTCATCAGCTTATACCCCTCCTGCTGACGCTATCCAATGGCAATGTGCTG
CGGCAGGTGCTACACCTAAAGTTACTGGTGCTACAGTAGGTACTTTACCTGCAAAACTTGCTCCTTCTAACTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)

Pilin (bacterial filament) (Pilin)

(34-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.217

100

0.566
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.026

100

0.503
  pilA2 Legionella pneumophila str. Paris

48.466

100

0.497
  pilA2 Legionella pneumophila strain ERS1305867

47.853

100

0.491
  comP Acinetobacter baylyi ADP1

41.772

99.371

0.415
  pilE Neisseria gonorrhoeae strain FA1090

37.349

100

0.39