Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CAL7507_RS08845 Genome accession   NC_019682
Coordinates   2033571..2035583 (+) Length   670 a.a.
NCBI ID   WP_015128123.1    Uniprot ID   K9PIE9
Organism   Calothrix sp. PCC 7507     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2028571..2040583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL7507_RS08835 (Cal7507_1852) dnaK 2030080..2032038 (-) 1959 WP_015128121.1 molecular chaperone DnaK -
  CAL7507_RS08840 (Cal7507_1853) grpE 2032306..2033046 (-) 741 WP_015128122.1 nucleotide exchange factor GrpE -
  CAL7507_RS08845 (Cal7507_1854) pilB/pilB1 2033571..2035583 (+) 2013 WP_015128123.1 GspE/PulE family protein Machinery gene
  CAL7507_RS08850 (Cal7507_1855) pilT 2035683..2036807 (+) 1125 WP_015128124.1 type IV pilus twitching motility protein PilT Machinery gene
  CAL7507_RS08855 (Cal7507_1856) - 2037035..2038258 (+) 1224 WP_015128125.1 type II secretion system F family protein -
  CAL7507_RS08860 (Cal7507_1858) - 2038479..2039045 (+) 567 WP_015128127.1 hypothetical protein -
  CAL7507_RS08865 (Cal7507_1859) - 2039394..2039624 (+) 231 WP_015128128.1 hypothetical protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74641.38 Da        Isoelectric Point: 5.1016

>NTDB_id=54706 CAL7507_RS08845 WP_015128123.1 2033571..2035583(+) (pilB/pilB1) [Calothrix sp. PCC 7507]
MTYSSPQRRSTALTTRTEFSPFGTKLVQAGYVNSDQMRQALVESRKSGTPLTEMLESITGRQLSPELLRQYKKQQLFELK
ILYGVESLDPEVNQLGNAMVGQLIETLIPVDICRRHRLVPLSKHEDQTPPCVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEQAVKQKHLDQEKFTDINQDLENLGNLDLEDAPDDMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIAAVTARFKIIASLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALAEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMISSSLIGVLAQRLVRRVCSDCRIAYTPTVEELARYGLSASQDVGVTFYKANSLTTEGVAEAKAKNQLCPTCNG
VGYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCNATLQPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54706 CAL7507_RS08845 WP_015128123.1 2033571..2035583(+) (pilB/pilB1) [Calothrix sp. PCC 7507]
ATGACTTACTCGTCACCACAACGCCGTAGTACCGCTCTCACCACAAGAACAGAGTTTTCGCCATTCGGCACCAAGCTAGT
ACAGGCTGGCTATGTTAATAGCGATCAGATGCGGCAGGCGCTGGTTGAAAGCCGCAAATCTGGCACACCTCTGACGGAAA
TGCTAGAGTCAATCACTGGGCGGCAACTGTCTCCTGAGTTACTCAGGCAATATAAGAAACAACAGTTATTTGAACTTAAA
ATATTATACGGTGTTGAATCCCTTGATCCGGAAGTCAACCAGCTTGGTAATGCTATGGTGGGGCAGCTAATTGAAACCCT
CATCCCAGTAGATATTTGCCGTCGTCATCGTTTAGTGCCACTTTCAAAACACGAAGATCAGACCCCCCCCTGTGTTTTAG
TGGCAATGGTCGATCCGGATAATCTAGAGGCTTCCGATGACCTCAATCGCATCTTGCGCCCACAAGGTTTAGCATTGCAG
CGCATGGTGATTACCCAGGAAGATTACCAGCAGTTAATCAATCAATACTTAGATGAACAGGCTGTTAAACAAAAACACTT
AGACCAAGAAAAGTTTACAGACATTAATCAGGATTTAGAAAATCTCGGAAATCTTGACTTAGAAGATGCACCCGATGACA
TGGAGGCTGATTTGGGTGCAGCGATGAAGGGTGCAGAGGATGCCCCAGTCATCAACTTAGTCAATAGAATCCTCGCCAAA
GCCTTGCATGAGAAGGTTTCTGATATTCACATTGAACCCCAAGAAGAAAACCTCCGCATTCGCTTTCGCAAGGATGGGGT
ACTGCGTGAAGCTTTTGACCCCCTACCGAAAAAAATCATTGCGGCGGTGACAGCCCGATTTAAAATCATCGCTAGTTTAG
ACATCGCTGAGAGGCGTTTACCCCAAGATGGACGCATCCGCCGCATGTTTGAGGGACGGAAGGTAGACTTCCGAGTCAAT
ACCTTACCCAGTCGTTATGGGGAAAAGGTGGTATTGCGGATTTTGGATAATTCCTCTACCCAACTAGGATTGAATAAGTT
AATCACCGACCCTGAGACTTTGCATATTGTTCAGGATATGGTGAGTCGTCCCTTCGGGTTGATTTTAGTAACTGGACCAA
CTGGTTCTGGTAAAACGACTTCGCTATATTCGGCTCTAGCAGAAAAGAACGATCCAGGAATTAACATCAGTACTGTAGAA
GACCCGATTGAGTACAGTTTGCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGATTGGATTTCGCTACGGCGCT
ACGGGCTTTCTTGCGACAAGATCCGGATGTCTTGTTGGTGGGTGAAACACGAGATAAAGAAACAGCAAAAACCGCGATTG
AAGCGGCGCTGACAGGTCACTTAGTATTAACTACCTTACATACTAATGACGCTCCTGGTGCGATCGCCCGTTTGGGAGAA
ATGGGTATTGAACCTTTCATGATTTCCAGTTCCTTAATAGGCGTTCTCGCACAGCGTCTAGTGCGACGCGTCTGTTCTGA
TTGTCGCATCGCCTATACCCCCACAGTTGAAGAACTGGCTCGCTACGGTTTATCAGCTTCTCAAGATGTAGGCGTCACTT
TCTATAAAGCCAACTCCTTGACAACAGAAGGCGTAGCAGAAGCCAAAGCCAAAAATCAGCTTTGCCCAACTTGTAATGGC
GTTGGTTACAAAGGACGCTGTGGTGTTTATGAAGTCATGCGCGTCACCGAAAACCTGCAAACCCTCATCAACGAAGACGC
ACCCACAGAACGTATTAAGGAAGTAGCAGTAGAAGAAGGCATGAAAACCTTATTAGCCTACAGCCTAGATTTAGTGCGTC
AAGGTGCTACCACCCTAGAAGAAGTCGAGCGCGTGACGTTTACTGATACGGGCTTAGAAGCCGAGTTAAAAGCCAAACGT
AAGAGTGGTCTGACATGCCGGACTTGCAACGCCACATTACAACCAGAATGGCTAGATTGCCCCTACTGTATGACATCGCG
CTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9PIE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.22

100

0.664

Multiple sequence alignment