Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   THEOS_RS02175 Genome accession   NC_019386
Coordinates   393878..394828 (-) Length   316 a.a.
NCBI ID   WP_016328706.1    Uniprot ID   K7QY60
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388878..399828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS02160 (Theos_0433) - 389837..390391 (-) 555 WP_016328703.1 shikimate kinase -
  THEOS_RS02165 (Theos_0434) aroC 390391..391542 (-) 1152 WP_016328704.1 chorismate synthase -
  THEOS_RS14250 pilQ 391617..393065 (-) 1449 WP_420802238.1 secretin N-terminal domain-containing protein Machinery gene
  THEOS_RS14255 - 393046..393120 (+) 75 WP_413217667.1 hypothetical protein -
  THEOS_RS02175 (Theos_0436) pilW 393878..394828 (-) 951 WP_016328706.1 hypothetical protein Machinery gene
  THEOS_RS02180 (Theos_0437) pilO 394825..395388 (-) 564 WP_187288203.1 type 4a pilus biogenesis protein PilO Machinery gene
  THEOS_RS02185 (Theos_0438) pilN 395429..396055 (-) 627 WP_016328708.1 hypothetical protein Machinery gene
  THEOS_RS02190 (Theos_0439) pilM 396048..397193 (-) 1146 WP_016328709.1 type IV pilus assembly protein PilM Machinery gene
  THEOS_RS02195 (Theos_0440) - 397378..398382 (-) 1005 WP_016328710.1 homoisocitrate dehydrogenase -
  THEOS_RS02200 (Theos_0441) - 398428..398934 (-) 507 WP_016328711.1 DUF5317 domain-containing protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 32553.67 Da        Isoelectric Point: 4.7757

>NTDB_id=54350 THEOS_RS02175 WP_016328706.1 393878..394828(-) (pilW) [Thermus oshimai JL-2]
MKEPERGTPTTLARFLSGLRNLPQSTKILLVALLLVGGVALWYVGLYLPAQGGPAAAEPPQATPTPGPIEAPPIPPLTPS
EEAKTEPQPQTQTQAPQAEPPKAEEPPRTVAPPRAVQATTPPPNPFVPLVVEAPAPPIQPQPVSPIPRGEPVVVAPPPRV
EVRPQAPLPTPTTGRTPLPGTAGALPAPKVLTPTARVETPALPQERVAPQAPLTLVEAPLPQQASPSAQEGVPPTPAPAP
KTPLEALVEEKGLKLSGTLLGPVSVAILESKDGYLVLPAGSPIPGSEAIVKGIEEGRVTLALKEERMDLTLSGGGE

Nucleotide


Download         Length: 951 bp        

>NTDB_id=54350 THEOS_RS02175 WP_016328706.1 393878..394828(-) (pilW) [Thermus oshimai JL-2]
GTGAAGGAACCAGAGCGGGGCACCCCAACGACCCTGGCCCGCTTCCTCTCGGGCCTGCGCAACCTGCCCCAGTCCACGAA
AATTCTCCTGGTAGCCCTCCTTCTGGTGGGAGGGGTAGCCCTGTGGTACGTAGGGCTCTACCTGCCGGCCCAGGGGGGGC
CGGCGGCGGCGGAACCCCCCCAGGCCACCCCCACCCCCGGCCCCATCGAGGCCCCCCCTATCCCGCCCCTCACCCCCAGC
GAGGAAGCCAAGACGGAGCCCCAGCCCCAGACCCAGACCCAAGCCCCTCAGGCCGAACCCCCAAAAGCCGAGGAGCCCCC
CAGGACCGTGGCGCCCCCACGGGCCGTTCAGGCCACCACCCCGCCGCCCAACCCCTTCGTGCCCCTGGTGGTGGAAGCCC
CCGCCCCGCCCATCCAGCCCCAGCCCGTAAGCCCCATCCCCAGGGGCGAGCCCGTGGTGGTGGCCCCCCCACCCCGGGTG
GAGGTCCGCCCGCAGGCCCCCCTGCCCACCCCTACCACCGGCCGTACCCCCTTGCCGGGGACCGCGGGGGCCTTGCCCGC
CCCTAAGGTCCTGACGCCCACGGCCCGGGTGGAAACCCCGGCCCTACCCCAAGAACGGGTGGCCCCCCAGGCCCCCCTCA
CCCTGGTGGAAGCGCCTTTACCCCAACAGGCGAGCCCTTCCGCACAGGAGGGGGTTCCCCCAACCCCAGCCCCTGCCCCC
AAAACCCCCCTCGAGGCCCTGGTGGAGGAAAAGGGCTTGAAGCTTTCGGGCACCCTCCTGGGCCCGGTGAGCGTGGCCAT
CCTGGAGAGCAAGGACGGGTACCTGGTGCTGCCCGCAGGCAGCCCCATCCCGGGTAGCGAAGCCATTGTGAAGGGTATTG
AGGAAGGCCGGGTTACGCTGGCCCTAAAGGAGGAACGTATGGACCTGACCCTATCGGGAGGTGGCGAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7QY60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

52.751

97.785

0.516

Multiple sequence alignment